scholarly journals Endocannabinoids increase human adipose stem cell differentiation and growth factor secretion in vitro

2020 ◽  
Author(s):  
Tim Ruhl ◽  
Pia‐Alina Schneider ◽  
Bong‐Sung Kim ◽  
Justus P. Beier
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Growth Factor ◽  
Stem Cell Differentiation ◽  
Adipose Stem Cell ◽  
Human Adipose Stem Cell ◽  
Factor Secretion ◽  
Growth Factor Secretion

scholarly journals Human adipose stem cell differentiation is highly affected by cancer cells both in vitro and in vivo: implication for autologous fat grafting

2017 ◽  
Vol 8 (1) ◽  
pp. e2568-e2568 ◽  
Author(s):  
Francesca Paino ◽  
Marcella La Noce ◽  
Diego Di Nucci ◽  
Giovanni Francesco Nicoletti ◽  
Rosa Salzillo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Fat Grafting ◽  
Adipose Stem Cell ◽  
Autologous Fat Grafting ◽  
Autologous Fat ◽  
Human Adipose Stem Cell

scholarly journals Effect of Decellularized Cartilage Bovine Scaffold and Hypoxic Condition on Stem Cell Differentiation to Chondrocyte: An In Vitro Study

2018 ◽  
Vol 35 ◽  
pp. 67-76
Author(s):  
Ferdiansyah Mahyudin ◽  
Dwikora Novembri Utomo ◽  
Tri Wahyu Martanto ◽  
Aries Rakhmat Hidayat ◽  
Linta Meyla Putri
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Growth Factor ◽  
Collagen Type ◽  
Hypoxic Condition ◽  
Stem Cell Differentiation ◽  
Type Ii ◽  
Collagen Type Ii

Autologous Chondrocyte Implantation (ACI) has been established for years to treat cartilage defect. Application of tissue engineering has advantages over ACI as tissue engineering requires simpler procedures without leaving morbidity at the donor site. Decellularized bovine cartilage scaffold and hypoxic stem cell differentiation were used in this in vitro experimental study. Comparative test was done between three study groups using bone marrow mesenchymal stem cells treated in three different conditions: growth factor-rich chondrogenic medium, scaffold without growth factor, and combination of both. Each group was given two oxygen tension conditions of normoxia and hypoxic within phase of stem cell differentiation. Immunohistochemical examinations on SOX9, RUNX2, and collagen type II were done for evaluation. After 5-week treatment, the result showed that the highest expression SOX9 and collagen type II were found within the group that used the combination of both scaffold and chondrogenic medium in hypoxic condition. Collagen type II expression in scaffold without additional growth factor showed no statistically significant difference compared with the combination group in hypoxic condition. Cartilage tissue engineering has proven its effectiveness for cartilage regeneration. Decellularized biomaterial scaffold limited the use of growth factor resulting in better cost and resource efficiency.

scholarly journals Chromobox Protein Homolog 3 Is Essential for Stem Cell Differentiation to Smooth Muscles In Vitro and in Embryonic Arteriogenesis

2011 ◽  
Vol 31 (8) ◽  
pp. 1842-1852 ◽  
Author(s):  
Qingzhong Xiao ◽  
Gang Wang ◽  
Xiaoke Yin ◽  
Zhenling Luo ◽  
Andriani Margariti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Smooth Muscles ◽  
Stem Cell Differentiation

Current and Future Trends in Adipose Stem Cell Differentiation into Neuroglia

2018 ◽  
Vol 36 (5) ◽  
pp. 230-240 ◽  
Author(s):  
Sajan George ◽  
Michael R. Hamblin ◽  
Heidi Abrahamse
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Future Trends ◽  
Adipose Stem Cell

scholarly journals Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments

2018 ◽  
Vol 2018 ◽  
pp. 1-15
Author(s):  
Quyen A. Tran ◽  
Visar Ajeti ◽  
Brian T. Freeman ◽  
Paul J. Campagnola ◽  
Brenda M. Ogle
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Early Time ◽  
Stem Cell Differentiation ◽  
Model Systems ◽  
Ecm Remodeling ◽  
Time Points ◽  
3D Microenvironment

Developmental studies and 3D in vitro model systems show that the production and engagement of extracellular matrix (ECM) often precede stem cell differentiation. Yet, unclear is how the ECM triggers signaling events in sequence to accommodate multistep process characteristic of differentiation. Here, we employ transcriptome profiling and advanced imaging to delineate the specificity of ECM engagement to particular differentiation pathways and to determine whether specificity in this context is a function of long-term ECM remodeling. To this end, human mesenchymal stem cells (hMSCs) were cultured in 3D bioprinted prisms created from ECM proteins and associated controls. We found that exogenous ECM provided in 3D microenvironments at early time points impacts on the composition of microenvironments at later time points and that each evolving 3D microenvironment is uniquely poised to promote stem cell differentiation. Moreover, 2D cultures undergo minimal ECM remodeling and are ill-equipped to stimulate pathways associated with development.

Sign in / Sign up

Export Citation Format

Share Document