Development of in vitro endothelialized drug‐eluting stent using human peripheral blood‐derived endothelial progenitor cells

2020 ◽  
Vol 14 (10) ◽  
pp. 1415-1427
Author(s):  
Jitsuro Tsukada ◽  
Frederic Wolf ◽  
Felix Vogt ◽  
Nicole Schaaps ◽  
Sven Thoröe‐Boveleth ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Drug Eluting Stent ◽  
Endothelial Progenitor ◽  
Drug Eluting

EFFECTS OF RESVERATROL ON NUMBER AND ACTIVITY OF ENDOTHELIAL PROGENITOR CELLS FROM HUMAN PERIPHERAL BLOOD

2007 ◽  
Vol 0 (0) ◽  
pp. 070626200939003-??? ◽  
Author(s):  
Xiao-Bin Wang ◽  
Jun Huang ◽  
Jian-Gang Zou ◽  
En-Ben Su ◽  
Qi-Jun Shan ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Endothelial Progenitor

scholarly journals EFFECT OF FLAVONOIDS ON OXIDATIVE STRESS, APOPTOSIS, AND CELL MARKERS OF PERIPHERAL BLOOD-DERIVED ENDOTHELIAL PROGENITOR CELLS: AN IN VITRO STUDY

2021 ◽  
pp. 39-42
Author(s):  
WAHYU WIDOWATI ◽  
RIMONTA F. GUNANEGARA ◽  
TERESA LILIANA WARGASETIA ◽  
HANNA SARI WIDYA KUSUMA ◽  
SEILA ARUMWARDANA ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
In Vitro Study ◽  
Endothelial Progenitor ◽  
Peripheral Blood Mononuclear ◽  
Cell Markers

Objective: Circulating EPCs (endothelial progenitor cells) play a role in neovascularization and vascular repair. Oxidative stress impairs endothelial progenitor. Flavonoid is a phytochemical compound for antioxidant activity. Flavonoid effects toward oxidative stress, apoptosis, and expression of the cell markers on EPCs are not fully understood. This study was aimed to elucidate the effects of quercetin, kaempferol, and myricetin toward oxidative stress, apoptosis, and cell markers of peripheral blood-derived-EPCs. Methods: EPCs (endothelial progenitor cells) were isolated from peripheral blood mononuclear cells (PBMNCs) using cultivation under EPCs spesific media. Oxidative stress in EPCs was induced by H2O2 and then treated by quercetin, kaempferol, and myricetin. Cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, while intracellular reactive oxygen species (ROS), apoptosis and characterization of cells, which expressed CD133 and KDR, was measured using flow cytometry. Results: Quercetin, kaempferol, and myricetin at concentration 12.50 µmol/l were not toxic on EPCs as the cells viability were 96.11±4.03%, 95.42±7.75%, and 94.22±9.49%, respectively. Flavonoids decreased intracellular ROS level in EPCs (quercetin: 14.38±1.47%, kaempferol: 20.21±6.25%, and myricetin: 13.88±4.02%) compared to EPCs treated with H2O2 (30.70%±1.04). Percetage of EPCs apoptosis was not significantly different among each treatment. Immunophenotyping showed the increasing of CD133 and KDR expression in EPCs treated with flavonoids. Conclusion: Quercetin, kaempferol, and myricetin were safe for EPCs, decreased ROS levels, and increased CD133 and KDR expression. However, the flavonoids did not significantly affect EPCs apoptosis.

Cord Blood Derived Endothelial Progenitor Cells Are More Immunosuppressive and Immunomodulator than Their Adult Peripheral Blood Counterparts

2021 ◽  
Author(s):  
Sina Naserian ◽  
Georges Uzan
Keyword(s):  
Cell Therapy ◽  
Cord Blood ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Immune Cells ◽  
Peripheral Blood ◽  
Endothelial Progenitor ◽  
Adult Peripheral Blood ◽  
Immunomodulatory Function

Endothelial progenitor cells (EPCs) are immature endothelial cells (ECs) present in blood circulation that are involved in neo-vascularization and correction of ischemic sites. Several cardiovascular disorders are correlated with patients inefficient and impaired EPCs, therefore, cell therapy using functional allogenic EPCs are considered as only alternative. Many studies show that cord blood (CB) yields much more EPCs than adult peripheral blood (APB), and these CB-EPCs are also more active. However, due to the reaction of host immune response to allogenic cells which usually lead to their rejection, we have investigated the exact impact of EPCs on immune cells. The pro-angiogenic and regenerative properties of EPCs have been already reported. However, little is known about their immunological features. Using different in-vitro combinations, we performed co-cultures of EPCs and T cells to investigate the interaction of EPCs and immune system. We demonstrated that both CB-EPCs APB-EPCs are able to suppress total PBMCs and among them T cell proliferation. In addition, our results reveal a more accentuated immunosuppressive and immunomodulatory function of CB-EPCs in comparison to APB-EPCs. This finding proves that CB-EPCs are more proper to cell therapy applications. Displaying both angiogenic and immunosuppressive properties make them the ideal choice for pathological conditions in which both functions are critical.

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