Noninvasive optical coherence tomography imaging of three‐dimensional cardiac tissues derived from human induced pluripotent stem cells

2020 ◽  
Vol 14 (10) ◽  
pp. 1384-1393
Author(s):  
Atsushi Miyaoka ◽  
Yoshinari Tsukamoto ◽  
Daisuke Takagi ◽  
Manabu Seo ◽  
Shigeru Miyagawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Optical Coherence Tomography ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Optical Coherence ◽  
Cardiac Tissues ◽  
Tomography Imaging ◽  
Optical Coherence Tomography Imaging ◽  
Induced Pluripotent

scholarly journals Novel hybrid three-dimensional artificial liver using human induced pluripotent stem cells and a rat decellularized liver scaffold

2019 ◽  
Vol 10 ◽  
pp. 127-133 ◽  
Author(s):  
Takahito Minami ◽  
Takamichi Ishii ◽  
Kentaro Yasuchika ◽  
Ken Fukumitsu ◽  
Satoshi Ogiso ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Artificial Liver ◽  
Induced Pluripotent ◽  
Decellularized Liver

scholarly journals Modeling Herpes Simplex Virus 1 Infections in Human Central Nervous System Neuronal Cells Using Two- and Three-Dimensional Cultures Derived from Induced Pluripotent Stem Cells

2019 ◽  
Vol 93 (9) ◽  
Author(s):  
Leonardo D’Aiuto ◽  
David C. Bloom ◽  
Jennifer N. Naciri ◽  
Adam Smith ◽  
Terri G. Edwards ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Herpes Simplex Virus 1 ◽  
Cns Neurons ◽  
Induced Pluripotent ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACTHerpes simplex virus 1 (HSV-1) establishes latency in both peripheral nerve ganglia and the central nervous system (CNS). The outcomes of acute and latent infections in these different anatomic sites appear to be distinct. It is becoming clear that many of the existing culture models using animal primary neurons to investigate HSV-1 infection of the CNS are limited and not ideal, and most do not recapitulate features of CNS neurons. Human induced pluripotent stem cells (hiPSCs) and neurons derived from them are documented as tools to study aspects of neuropathogenesis, but few have focused on modeling infections of the CNS. Here, we characterize functional two-dimensional (2D) CNS-like neuron cultures and three-dimensional (3D) brain organoids made from hiPSCs to model HSV-1–human–CNS interactions. Our results show that (i) hiPSC-derived CNS neurons are permissive for HSV-1 infection; (ii) a quiescent state exhibiting key landmarks of HSV-1 latency described in animal models can be established in hiPSC-derived CNS neurons; (iii) the complex laminar structure of the organoids can be efficiently infected with HSV, with virus being transported from the periphery to the central layers of the organoid; and (iv) the organoids support reactivation of HSV-1, albeit less efficiently than 2D cultures. Collectively, our results indicate that hiPSC-derived neuronal platforms, especially 3D organoids, offer an extraordinary opportunity for modeling the interaction of HSV-1 with the complex cellular and architectural structure of the human CNS.IMPORTANCEThis study employed human induced pluripotent stem cells (hiPSCs) to model acute and latent HSV-1 infections in two-dimensional (2D) and three-dimensional (3D) CNS neuronal cultures. We successfully established acute HSV-1 infections and infections showing features of latency. HSV-1 infection of the 3D organoids was able to spread from the outer surface of the organoid and was transported to the interior lamina, providing a model to study HSV-1 trafficking through complex neuronal tissue structures. HSV-1 could be reactivated in both culture systems; though, in contrast to 2D cultures, it appeared to be more difficult to reactivate HSV-1 in 3D cultures, potentially paralleling the low efficiency of HSV-1 reactivation in the CNS of animal models. The reactivation events were accompanied by dramatic neuronal morphological changes and cell-cell fusion. Together, our results provide substantive evidence of the suitability of hiPSC-based neuronal platforms to model HSV-1–CNS interactions in a human context.

scholarly journals Three-dimensional High-speed Optical Coherence Tomography Imaging of Lamina Cribrosa in Glaucoma

Ophthalmology ◽  
2009 ◽  
Vol 116 (2) ◽  
pp. 214-222 ◽  
Author(s):  
Ryo Inoue ◽  
Masanori Hangai ◽  
Yuriko Kotera ◽  
Hideo Nakanishi ◽  
Satoshi Mori ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Speed ◽  
Three Dimensional ◽  
Lamina Cribrosa ◽  
Optical Coherence ◽  
Tomography Imaging ◽  
Optical Coherence Tomography Imaging

scholarly journals Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused Three-Dimensional Multicompartment Bioreactor

2016 ◽  
Vol 5 (1) ◽  
pp. 235-248 ◽  
Author(s):  
Nora Freyer ◽  
Fanny Knöspel ◽  
Nadja Strahl ◽  
Leila Amini ◽  
Petra Schrade ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Hepatic Differentiation ◽  
Induced Pluripotent

Three-dimensional functional human myocardial tissues fabricated from induced pluripotent stem cells

2015 ◽  
Vol 11 (3) ◽  
pp. 926-935 ◽  
Author(s):  
Hyoe Komae ◽  
Hidekazu Sekine ◽  
Izumi Dobashi ◽  
Katsuhisa Matsuura ◽  
Minoru Ono ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Induced Pluripotent

scholarly journals Tubular Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells Generate Pulse Pressure In Vivo

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Hiroyoshi Seta ◽  
Katsuhisa Matsuura ◽  
Hidekazu Sekine ◽  
Kenji Yamazaki ◽  
Tatsuya Shimizu
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pulse Pressure ◽  
Pluripotent Stem Cells ◽  
Cardiac Tissues ◽  
Induced Pluripotent
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