scholarly journals Bioengineering and in utero transplantation of fetal skin in the sheep model: A crucial step towards clinical application in human fetal spina bifida repair

2019 ◽  
Vol 14 (1) ◽  
pp. 58-65 ◽  
Author(s):  
Luca Mazzone ◽  
Ueli Moehrlen ◽  
Nicole Ochsenbein‐Kölble ◽  
Luca Pontiggia ◽  
Thomas Biedermann ◽  
...  
Keyword(s):  
Spina Bifida ◽  
Clinical Application ◽  
In Utero ◽  
Fetal Skin ◽  
Sheep Model ◽  
Crucial Step ◽  
In Utero Transplantation

scholarly journals Neurological Outcomes after Human Umbilical Cord Patch for In Utero Spina Bifida Repair in a Sheep Model

2016 ◽  
Vol 06 (03) ◽  
pp. e309-e317 ◽  
Author(s):  
Lovepreet Mann ◽  
Saul Snowise ◽  
Yisel Morales ◽  
Sanjay Prabhu ◽  
Scheffer Tseng ◽  
...  
Keyword(s):  
Spina Bifida ◽  
Umbilical Cord ◽  
In Utero ◽  
Human Umbilical Cord ◽  
Sheep Model ◽  
Neurological Outcomes

scholarly journals 60: Cryopreserved umbilical cord as a meningeal patch for in-utero spina bifida repair in sheep model

2019 ◽  
Vol 220 (1) ◽  
pp. S49
Author(s):  
Ramesha Papanna ◽  
Lovepreet K. Mann ◽  
Jong H. Won ◽  
Thai Vu ◽  
Rajan Patel ◽  
...  
Keyword(s):  
Spina Bifida ◽  
Umbilical Cord ◽  
In Utero ◽  
Sheep Model

Homing of Human Cells in the Fetal Sheep Model: Modulation by Antibodies Activating or Inhibiting Very Late Activation Antigen-4–Dependent Function

Blood ◽  
1999 ◽  
Vol 94 (7) ◽  
pp. 2515-2522 ◽  
Author(s):  
Esmail D. Zanjani ◽  
Alan W. Flake ◽  
Graça Almeida-Porada ◽  
Nam Tran ◽  
Thalia Papayannopoulou
Keyword(s):  
Molecular Mechanisms ◽  
Fetal Liver ◽  
In Utero ◽  
Human Cells ◽  
Human Donor ◽  
Sheep Model ◽  
Fetal Sheep ◽  
In Utero Transplantation ◽  
Donor Cells ◽  
Activation Antigen

The mechanisms by which intravenously (IV)-administered hematopoietic cells home to the bone marrow (BM) are poorly defined. Although insightful information has been obtained in mice, our knowledge about homing of human cells is very limited. In the present study, we investigated the importance of very late activation antigen (VLA)-4 in the early phases of lodgment of human CD34+progenitors into the sheep hematopoietic compartment after in utero transplantation. We have found that preincubation of donor cells with anti–VLA-4 blocking antibodies resulted in a profound reduction of human cell lodgment in the fetal BM at 24 and 48 hours after transplantation, with a corresponding increase of human cells in the peripheral circulation. Furthermore, IV infusion of the anti–VLA-4 antibody at later times (posttransplantation days 21 to 24) resulted in redistribution or mobilization of human progenitors from the BM to the peripheral blood. In an attempt to positively modulate homing, we also pretreated human donor cells with an activating antibody to β1 integrins. This treatment resulted in increased lodgment of donor cells in the fetal liver, presumably for hemodynamic reasons, at the expense of the BM. Given previous involvement of the VLA-4/vascular cell adhesion molecule (VCAM)-1 adhesion pathway in homing and mobilization in the murine system, our present data suggest that cross-reacting ligands (likely VCAM-1) for human VLA-4 exist in sheep BM, thereby implicating conservation of molecular mechanisms of homing and mobilization across disparate species barriers. Thus, information from xenogeneic models of human hematopoiesis and specifically, the human/sheep model of in utero transplantation, may provide valuable insights into human hematopoietic transplantation biology.

scholarly journals 93: Tissue graft materials for skin patch during in-utero spina bifida repair in a sheep model

2020 ◽  
Vol 222 (1) ◽  
pp. S77
Author(s):  
Lovepreet Mann ◽  
Jong Won ◽  
Stephen A. Fletcher ◽  
Ranu Jain ◽  
Rajan Patel ◽  
...  
Keyword(s):  
Spina Bifida ◽  
In Utero ◽  
Sheep Model ◽  
Tissue Graft ◽  
Skin Patch

52: Neurological outcomes after cryopreserved human umbilical cord (HUC) patch for in-utero spina bifida repair in a sheep model

2016 ◽  
Vol 214 (1) ◽  
pp. S37
Author(s):  
Ramesha Papanna ◽  
Lovepreet K. Mann ◽  
Saul Snowise ◽  
Roopali Donepudi ◽  
Yisel Morales ◽  
...  
Keyword(s):  
Spina Bifida ◽  
Umbilical Cord ◽  
In Utero ◽  
Human Umbilical Cord ◽  
Sheep Model ◽  
Neurological Outcomes

scholarly journals 85: Conventional vs cryopreserved human umbilical cord (HUC) patch based repair for in-utero spina bifida in a sheep model

2017 ◽  
Vol 216 (1) ◽  
pp. S61-S62
Author(s):  
Ramesha Papanna ◽  
Lovepreet K. Mann ◽  
Jong H. Won ◽  
Yisel Morales ◽  
Stephen Fletcher ◽  
...  
Keyword(s):  
Spina Bifida ◽  
Umbilical Cord ◽  
In Utero ◽  
Human Umbilical Cord ◽  
Sheep Model

scholarly journals 81 A three-layer fetoscopic spina bifida closure has fewer neonatal complications than open in-utero closure

2021 ◽  
Vol 224 (2) ◽  
pp. S56-S57
Author(s):  
Jimmy Espinoza ◽  
Michael A. Belfort ◽  
William Whitehead ◽  
Magdalena Sanz-Cortes ◽  
Ahmed A. Nassr ◽  
...  
Keyword(s):  
Spina Bifida ◽  
In Utero ◽  
Neonatal Complications

In Utero Transplantation of Human Cord Blood Cells into Rabbits

2005 ◽  
Vol 80 (2) ◽  
pp. 282-283 ◽  
Author(s):  
Georg S. Wengler ◽  
Guerino Lombardi ◽  
Tiziana Frusca ◽  
Daniele Alberti ◽  
Alberto Albertini ◽  
...  
Keyword(s):  
Cord Blood ◽  
Blood Cells ◽  
In Utero ◽  
Human Cord Blood ◽  
In Utero Transplantation ◽  
Cord Blood Cells
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