Effect of the small compound TD ‐198946 on glycosaminoglycan synthesis and transforming growth factor β3‐associated chondrogenesis of human synovium‐derived stem cells in vitro

2019 ◽  
Vol 13 (3) ◽  
pp. 446-458 ◽  
Author(s):  
Ryota Chijimatsu ◽  
Fumiko Yano ◽  
Taku Saito ◽  
Masato Kobayashi ◽  
Shuichi Hamamoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Glycosaminoglycan Synthesis ◽  
Small Compound

scholarly journals DPSCs treated by TGF-β1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signaling

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yuchen Zhang ◽  
Junqing Liu ◽  
Ting Zou ◽  
Yubingqing Qi ◽  
Baicheng Yi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Blood Vessels ◽  
Western Blotting ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Vegf Receptor ◽  
Vegfr2 Signaling ◽  
Tgf Β1

Abstract Background Maintaining the stability and maturation of blood vessels is of paramount importance for the vessels to carry out their physiological function. Smooth muscle cells (SMCs), pericytes, and mesenchymal stem cells (MSCs) are involved in the maturation process of the newly formed vessels. The aim of this study was to investigate whether transforming growth factor beta 1 (TGF-β1) treatment could enhance pericyte-like properties of dental pulp stem cells (DPSCs) and how TGF-β1-treated DPSCs for 7 days (T-DPSCs) stabilize the newly formed blood vessels. Methods We utilized TGF-β1 to treat DPSCs for 1, 3, 5, and 7 days. Western blotting and immunofluorescence were used to analyze the expression of SMC markers. Functional contraction assay was conducted to assess the contractility of T-DPSCs. The effects of T-DPSC-conditioned media (T-DPSC-CM) on human umbilical vein endothelial cell (HUVEC) proliferation and migration were examined by MTT, wound healing, and trans-well migration assay. Most importantly, in vitro 3D co-culture spheroidal sprouting assay was used to investigate the regulating role of vascular endothelial growth factor (VEGF)-angiopoietin (Ang)-Tie2 signaling on angiogenic sprouting in 3D co-cultured spheroids of HUVECs and T-DPSCs. Angiopoietin 2 (Ang2) and VEGF were used to treat the co-cultured spheroids to explore their roles in angiogenic sprouting. Inhibitors for Tie2 and VEGFR2 were used to block Ang1/Tie2 and VFGF/VEGFR2 signaling. Results Western blotting and immunofluorescence showed that the expression of SMC-specific markers (α-SMA and SM22α) were significantly increased after treatment with TGF-β1. Contractility of T-DPSCs was greater compared with that of DPSCs. T-DPSC-CM inhibited HUVEC migration. In vitro sprouting assay demonstrated that T-DPSCs enclosed HUVECs, resembling pericyte-like cells. Compared to co-culture with DPSCs, a smaller number of HUVEC sprouting was observed when co-cultured with T-DPSCs. VEGF and Ang2 co-stimulation significantly enhanced sprouting in HUVEC and T-DPSC co-culture spheroids, whereas VEGF or Ang2 alone exerted insignificant effects on HUVEC sprouting. Blocking Tie2 signaling reversed the sprouting inhibition by T-DPSCs, while blocking VEGF receptor (VEGFR) signaling boosted the sprouting inhibition by T-DPSCs. Conclusions This study revealed that TGF-β1 can induce DPSC differentiation into functional pericyte-like cells. T-DPSCs maintain vessel stability through Ang1/Tie2 and VEGF/VEGFR2 signaling.

Mouse embryonic stem cells with aberrant transforming growth factor β signalling exhibit impaired differentiation in vitro and in vivo

1998 ◽  
Vol 63 (3) ◽  
pp. 101-113 ◽  
Author(s):  
Marie-José Goumans ◽  
Dorien Ward-van Oostwaard ◽  
Florence Wianny ◽  
Pierre Savatier ◽  
An Zwijsen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Embryonic Stem ◽  
Transforming Growth Factor Β ◽  
Mouse Embryonic Stem Cells

scholarly journals Comparative impact of platelet rich plasma and transforming growth factor-β on chondrogenic differentiation of human adipose derived stem cells

Bioimpacts ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Roya Hesari ◽  
Mina Keshvarinia ◽  
Mahboubeh Kabiri ◽  
Iman Rad ◽  
Kazem Parivar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Side Effects ◽  
Transforming Growth Factor Beta ◽  
Chondrogenic Differentiation ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Necrosis Factor Alpha

<span style="color: #1f497d;">Introduction: Transforming growth factor-beta (TGF-β) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors. On the other hand, platelet-rich plasma (PRP) is found to have a chondrogenic impact on mesenchymal stem cell proliferation and differentiation, with no considerable side effects. Therefore, we compared chondrogenic impact of TGF-β and PRP on adipose-derived stem cells (ADSCs), to see if PRP could be introduced as an alternative to TGF-β. <br /> <span style="color: #1f497d;">Methods: Differentiation of ADSCs was monitored using a couple of methods including glycosaminoglycan production, miRNAs expression, vascular endothelial growth factor (VEGF)/tumor necrosis factor alpha (TNFα) secretion, alkaline phosphatase (ALP) and calcium content assays.<br /> <span style="color: #1f497d;">Results: Accordingly, the treatment of differentiating cells with 5% (v/v) PRP resulted in higher glycosaminoglycan production, enhanced SOX9 transcription, and lowered TNFα and VEGF secretion compared to the control and TGF-β groups. Besides, the application of PRP to the media up-regulated miR-146a and miR-199a in early and late stages of chondrogenesis, respectively. <span style="color: #1f497d;">Conclusion: PRP induces in vitro chondrogenesis, as well as TGF-β with lesser inflammatory and hypertrophic side effects.

Dose- and Time-Dependent Effects of Cyclic Hydrostatic Pressure on Transforming Growth Factor-3-Induced Chondrogenesis by Adult Human Mesenchymal Stem Cells in Vitro

2006 ◽  
Vol 0 (0) ◽  
pp. 060706073730066
Author(s):  
Keita Miyanishi ◽  
Michael C.D. Trindade ◽  
Derek P. Lindsey ◽  
Gary S. Beaupre ◽  
Dennis R. Carter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Hydrostatic Pressure ◽  
Transforming Growth Factor ◽  
Human Mesenchymal Stem Cells ◽  
Time Dependent ◽  
Adult Human

Construction of a Blood Vessel Wall with Adipose-Derived Stem Cell Sheets In Vitro

2021 ◽  
Vol 11 (8) ◽  
pp. 1452-1458
Author(s):  
Zhicheng Xu ◽  
Qun Zhang
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Adult Stem Cells ◽  
Transforming Growth Factor ◽  
Polyglycolic Acid ◽  
Large Vessel ◽  
Elastic Fibers ◽  
Control Group ◽  
Cell Sheets

Adipose-derived stem cells (ASCs) are a reliable and easily accessible source of adult stem cells. Thus, ASCs are increasingly used as seed cells in tissue engineering. This report investigated the possibility of engineering a large vessel using unwoven polyglycolic acid (PGA) fibers covered with ASC sheets treated with transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor-BB (PDGF-BB). After coiling around a silicone tube, the complex was secured by surgical sutures and cultured In Vitro for six weeks. Gross views showed that glossy and smooth tubular tissue was engineered. Histological examination revealed regularly distributed cells and a few collagenous and elastic fibers in the differentiated group. Cells were randomly distributed, and disorganized collagenous and small elastic fibers were detected in the control group. Our results indicate the promising potential of ASC sheets and PGA fibers for the construction of large vessel walls.

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