Comparative evaluation of healing biomarkers in skin wound exudates using a nanobiosensor and histological analysis of full‐thickness skin wounds grafted with multidirectional or unidirectional artificial dermis

2018 ◽  
Vol 12 (12) ◽  
pp. 2299-2308 ◽  
Author(s):  
Sergio Casadiegos ◽  
Rosa Helena Bustos ◽  
Marta R. Fontanilla
Keyword(s):  
Comparative Evaluation ◽  
Histological Analysis ◽  
Skin Wound ◽  
Skin Wounds ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Artificial Dermis ◽  
Thickness Skin

scholarly journals FIBROIN SILK BASED FILMS FOR RAT’S FULL-THICKNESS SKIN WOUND REGENERATION

2016 ◽  
Vol 18 (3) ◽  
pp. 74-84
Author(s):  
L. A. Safonova ◽  
M. M. Bobrova ◽  
O. I. Agapova ◽  
A. Yu. Arkhipova ◽  
A. V. Goncharenko ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Acid Solution ◽  
Skin Wound ◽  
Cytotoxicity Test ◽  
Skin Wounds ◽  
Full Thickness ◽  
Casting Method ◽  
Formic Acid Solution ◽  
Full Thickness Skin ◽  
Thickness Skin

Aimof this study is to research an effect of silk fi broin fi lms fabricated by casting method upon Wistar rat’s full-thickness skin wound regeneration.Materials and methods.4 different kinds of fi lms with protein concentration equal to 20 mg/ml were fabricated: fi lms from silk fi broin aqueous solution, fi lms from silk fi broin formic acid solution, fi lms from silk fi broin aqueous solution containing 30% collagen by weight, fi lms from silk fi broin formic acid solution containing 30% collagen by weight. All kinds of fi lms were fabricated by casting method on polished Tefl on surface. Scanning electron microscopy was applied to research fi lms’ surface structure. Cytotoxicity test of the fi lms was realized on mouse 3T3 fi broblasts model by MTT assay. Manufactured fi lms were utilized to regenerate full-thickness skin wounds in Wistar rats.Results.It was shown that fi lms’ surface was characterized by micro- and nanorelief in the form of roughness. The proliferative activity of mouse 3T3 fi broblasts increased during 7 days of cytotoxicity test. Fabricated fi lms enlarge the regeneration rate of full-thickness Wistar rat skin wounds an average of 25%. Histological analysis indicated structural skin restoration without any infl ammatory tissue.Conclusion.All fabricated fi lms are non-cytotoxic and characterized by appropriate structure for the adhesion and proliferation of fi broblasts. The application of fi lms for full-thickness skin wound regeneration increases its restoration rate which is confi rmed by histological examination. 

scholarly journals Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Poonam Shakya ◽  
A. K. Sharma ◽  
Naveen Kumar ◽  
Remya Vellachi ◽  
Dayamon D. Mathew ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Repair ◽  
Healing Process ◽  
Skin Wound ◽  
Skin Wounds ◽  
Full Thickness ◽  
Open Wound ◽  
Full Thickness Skin ◽  
Group I ◽  
Thickness Skin

An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm2) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P<0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.

Effect of Fibrin-binding Synthetic Oligopeptide on the Healing of Full-thickness Skin Wounds in Streptozotocin-induced Diabetic Rats

2012 ◽  
Vol 19 (7) ◽  
pp. 1321-1328
Author(s):  
Jae-Eun Chung ◽  
Yun-Jeong Kim ◽  
Yoon-Jeong Park ◽  
Ki-Tae Koo ◽  
Yang-Jo Seol ◽  
...  
Keyword(s):  
Diabetic Rats ◽  
Skin Wounds ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

Nanofiber-acellular dermal matrix as a bilayer scaffold containing mesenchymal stem cell for healing of full-thickness skin wounds

2018 ◽  
Vol 375 (3) ◽  
pp. 709-721 ◽  
Author(s):  
Mohamad Javad Mirzaei-parsa ◽  
Hossein Ghanbari ◽  
Behnam Alipoor ◽  
Amirhossein Tavakoli ◽  
Mohammad Reza H. Najafabadi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Acellular Dermal Matrix ◽  
Skin Wounds ◽  
Full Thickness ◽  
Dermal Matrix ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Acellular Dermal
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