scholarly journals Differentiation of human-induced pluripotent stem cell under flow conditions to mature hepatocytes for liver tissue engineering

2018 ◽  
Vol 12 (5) ◽  
pp. 1273-1284 ◽  
Author(s):  
Viktoriia Starokozhko ◽  
Mette Hemmingsen ◽  
Layla Larsen ◽  
Soumyaranjan Mohanty ◽  
Marjolijn Merema ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Liver Tissue ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Flow Conditions ◽  
Liver Tissue Engineering ◽  
Induced Pluripotent

scholarly journals Electrospun Scaffolds and Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Cardiac Tissue Engineering Applications

2020 ◽  
Vol 7 (3) ◽  
pp. 105
Author(s):  
Taylor Cook Suh ◽  
Alaowei Y. Amanah ◽  
Jessica M. Gluck
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cardiac Tissue ◽  
Complex Structure ◽  
Functional Limitation ◽  
Induced Pluripotent Stem Cell ◽  
Cardiac Cells ◽  
Electrospun Scaffolds ◽  
Induced Pluripotent

Tissue engineering (TE) combines cells, scaffolds, and growth factors to assemble functional tissues for repair or replacement of tissues and organs. Cardiac TE is focused on developing cardiac cells, tissues, and structures—most notably the heart. This review presents the requirements, challenges, and research surrounding electrospun scaffolds and induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CMs) towards applications to TE hearts. Electrospinning is an attractive fabrication method for cardiac TE scaffolds because it produces fibers that demonstrate the optimal potential for mimicking the complex structure of the cardiac extracellular matrix (ECM). iPSCs theoretically offer the capacity to generate limitless numbers of CMs for use in TE hearts, however these iPSC-CMs are electrophysiologically, morphologically, mechanically, and metabolically immature compared to adult CMs. This presents a functional limitation to their use in cardiac TE, and research aiming to address this limitation is presented in this review.

Abstract P359: Electroconductive Scaffolds To Mature Induced Pluripotent Stem Cell-derived Cardiomyocytes For Cardiac Tissue Engineering

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Suh Hee T Cook ◽  
Jessica Gluck
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cardiac Tissue ◽  
Induced Pluripotent Stem Cell ◽  
Cardiac Tissue Engineering ◽  
Patient Specific ◽  
Sem Images ◽  
Induced Pluripotent ◽  
Electrical Pacing

Heart disease is the leading cause of death worldwide. Cardiac tissue engineering (CTE) aims to repair and replace heart tissue, offering a solution. Induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CMs) could revolutionize CTE due to their theoretical ability to supply limitless patient-specific CMs. However, iPSC-CMs are electrophysiologically immature compared to functional adult CMs, and therefore incapable of sustaining a heartbeat. Thus, a scaffold capable of electrophysiologically maturing iPSC-CMs is needed. My research increases the electroconductivity of electrospun (ES) scaffolds by incorporating carbon nanotubes (CNTs), which I hypothesize will mature iPSC-CMs seeded onto them due to their excellent electroconductive properties. Morphological, biocompatibility, and electrical analyses have been performed on ES polycaprolactone (PCL) and gelatin scaffolds with CNTs incorporated via a ‘sandwich’ and dual deposition method in order to increase electroconductivity. Morphological analyses were performed via ImageJ on SEM images. Fiber diameter and pore size quantification confirmed the ability to exert morphological control by modifying solution properties and ES parameters, which is crucial to achieve biomimicry of the cardiac extracellular matrix. Live/dead assays and immunofluorescence revealed the CNT scaffolds offer high biocompatibility for NIH 3T3 fibroblasts, which attach, proliferate, and migrate well. Electrical analysis performed with a multimeter and two-probe resistance measurement confirms that inclusion of CNTs significantly increases scaffold conductivity, moreso for dual deposition scaffolds than ‘sandwich’ ones, and moreso parallel to the CNT arrays than orthogonally. These results prove the feasibility of using such scaffolds as a method for in vitro electrophysiological iPSC-CM maturation. Next steps include optimization of scaffolds, analysis of iPSC-CM biocompatibility and response, and recapitulation and manipulation of the electrophysiology of cardiac tissue, including quantification of markers for cardiac function and maturity, and assessment of iPSC-CM + scaffold response to electrical pacing.

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