An intervertebral disc whole organ culture system to investigate proinflammatory and degenerative disc disease condition

2018 ◽  
Vol 12 (4) ◽  
pp. e2051-e2061 ◽  
Author(s):  
Gernot Lang ◽  
Yishan Liu ◽  
Janna Geries ◽  
Zhiyu Zhou ◽  
David Kubosch ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Degenerative Disc Disease ◽  
Culture System ◽  
Disc Disease ◽  
Degenerative Disc ◽  
Organ Culture System ◽  
Disease Condition

scholarly journals An organ culture system to model early degenerative changes of the intervertebral disc II: profiling global gene expression changes

2013 ◽  
Vol 15 (5) ◽  
pp. R121 ◽  
Author(s):  
Dessislava Z Markova ◽  
Christopher K Kepler ◽  
Sankar Addya ◽  
Hallie B Murray ◽  
Alexander R Vaccaro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Global Gene Expression ◽  
Degenerative Changes ◽  
Organ Culture System

scholarly journals Development of an intact intervertebral disc organ culture system in which degeneration can be induced as a prelude to studying repair potential

2011 ◽  
Vol 20 (8) ◽  
pp. 1244-1254 ◽  
Author(s):  
Bernice Jim ◽  
Thomas Steffen ◽  
Janet Moir ◽  
Peter Roughley ◽  
Lisbet Haglund
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

scholarly journals Cell-Based Therapies Used to Treat Lumbar Degenerative Disc Disease: A Systematic Review of Animal Studies and Human Clinical Trials

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
David Oehme ◽  
Tony Goldschlager ◽  
Peter Ghosh ◽  
Jeffrey V. Rosenfeld ◽  
Graham Jenkin
Keyword(s):  
Stem Cells ◽  
Clinical Trials ◽  
Low Back Pain ◽  
Back Pain ◽  
Intervertebral Disc ◽  
Degenerative Disc Disease ◽  
Low Back ◽  
Disc Disease ◽  
Degenerative Disc ◽  
Disc Regeneration

Low back pain and degenerative disc disease are a significant cause of pain and disability worldwide. Advances in regenerative medicine and cell-based therapies, particularly the transplantation of mesenchymal stem cells and intervertebral disc chondrocytes, have led to the publication of numerous studies and clinical trials utilising these biological therapies to treat degenerative spinal conditions, often reporting favourable outcomes. Stem cell mediated disc regeneration may bridge the gap between the two current alternatives for patients with low back pain, often inadequate pain management at one end and invasive surgery at the other. Through cartilage formation and disc regeneration or via modification of pain pathways stem cells are well suited to enhance spinal surgery practice. This paper will systematically review the current status of basic science studies, preclinical and clinical trials utilising cell-based therapies to repair the degenerate intervertebral disc. The mechanism of action of transplanted cells, as well as the limitations of published studies, will be discussed.

scholarly journals Chemometric evaluation of concentrations of trace elements in intervertebral disc tissue in patient with degenerative disc disease

2017 ◽  
Vol 24 (4) ◽  
pp. 610-617
Author(s):  
Łukasz Kubaszewski ◽  
Anetta Zioła-Frankowska ◽  
Zuzanna Gasik ◽  
Marcin Frankowski ◽  
Mikołaj Dąbrowski ◽  
...  
Keyword(s):  
Trace Elements ◽  
Intervertebral Disc ◽  
Degenerative Disc Disease ◽  
Disc Disease ◽  
Degenerative Disc ◽  
Disc Tissue

Development of an Organ Culture System for Long-Term Survival of the Intact Human Intervertebral Disc

Spine ◽  
2011 ◽  
Vol 36 (22) ◽  
pp. 1835-1842 ◽  
Author(s):  
Rahul Gawri ◽  
Fackson Mwale ◽  
Jean Ouellet ◽  
Peter J. Roughley ◽  
Thomas Steffen ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Term Survival ◽  
Long Term Survival ◽  
Organ Culture System

scholarly journals HISTOLOGICAL MARKERS OF DEGENERATION AND REGENERATION OF THE HUMAN INTERVERTEBRAL DISK

2017 ◽  
Vol 16 (1) ◽  
pp. 42-47
Author(s):  
MANUELA PELETTI-FIGUEIRÓ ◽  
ISRAEL SILVEIRA DE AGUIAR ◽  
SUELEN PAESI ◽  
DENISE CANTARELLI MACHADO ◽  
SERGIO ECHEVERRIGARAY ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Degenerative Disc Disease ◽  
Intervertebral Disc Degeneration ◽  
Disc Disease ◽  
Lumbar Degenerative Disc Disease ◽  
Degenerative Disc ◽  
Hematoxylin And Eosin ◽  
Safranin O

ABSTRACT Objective: To define histological scores for intervertebral disc degeneration that would enable the definition of morphological characteristics of disease, besides improving knowledge of the lumbar degenerative disc disease by means of immunohistochemical markers. Methods: Hematoxylin and Eosin, Alcian/PAS, Masson Trichrome and Safranin O/FCF staining was used on the intervertebral disc degeneration sections of patients with lumbar degenerative disc disease. The protein markers defined in immunohistochemistry were cell proliferation (Ki-67) and apoptosis (p53). Results: The study data enabled the determination of Safranin O/FCF stain as the most effective one for evaluating parameters such as area, diameter, and number of chondrocyte clusters. The importance of using stains in association, such as Safranin O/FCF, Masson Trichrome, Alcian/PAS and Hematoxylin and Eosin, was also determined, as they are complementary for the histopathological verification of intervertebral disc degeneration. By expressing proteins using the immunohistochemistry technique, it was possible to consider two stages of disc degeneration: cell proliferation with chondrocyte cluster formation, and induction of apoptosis. Conclusion: This study enabled the histological and immunohistochemical characterization to be determined for lumbar degenerative disc disease, and its degrees of evolution, by determining new disc degeneration scores.

scholarly journals Development of a Whole Bovine Long-term Organ Culture System that Retains Vertebral Bone for Intervertebral Disc Repair and Biomechanical Studies using PrimeGrowth Media

2016 ◽  
Vol 6 (1_suppl) ◽  
pp. s-0036-1582897-s-0036-1582897
Author(s):  
Michael Grant ◽  
Laura Epure ◽  
Omar Salem ◽  
Motaz Alaqeel ◽  
John Antoniou ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Vertebral Bone ◽  
Organ Culture System ◽  
Disc Repair ◽  
Intervertebral Disc Repair

4:2898. Chondrocyte Based Gene Therapy for the Degenerating Intervertebral Disc in the Rabbit Disc Organ Culture System

2006 ◽  
Vol 6 (5) ◽  
pp. 48S
Author(s):  
Yejia Zhang ◽  
Howard S. An ◽  
Eugene Thonar ◽  
Theodore Oegema ◽  
Gunnar Andersson ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Intervertebral Disc ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

Pulmonary Impairment Rating

2016 ◽  
Vol 21 (4) ◽  
pp. 8-11
Author(s):  
Jay Blaisdell ◽  
James B. Talmage ◽  
Stephen Demeter
Keyword(s):  
Intervertebral Disc ◽  
Degenerative Disc Disease ◽  
Spinal Pain ◽  
Clinical Syndrome ◽  
Intervertebral Disc Herniation ◽  
Disc Disease ◽  
Degenerative Disc ◽  
Motion Segment ◽  
Objective Evidence ◽  
Disc Herniations

Abstract Nonspecific spinal pain and intervertebral disc herniations are common, and in evaluating spinal impairment physicians should carefully assess the significance of imaging findings, physical examination findings, and reports of limb pain. A case example illustrates key principles in assessing cervical pain in an individual with questionable arm complaints. A 62-year-old man had a slip and fall injury. Imaging studies revealed degenerative disc disease with disc bulges and without specific disc herniations according to the radiologists, but his physician reviewed magnetic resonance imaging (MRI) films and reported multiple disc herniations. The case example illustrates the significance of the finding of degenerative disc disease, determining whether to rate for “soft tissue and nonspecific conditions” or “motion segment lesions,” and assessing “nonverifiable radicular complaints.” The authors note that cervical degenerative disc “disease” is more aptly a radiologic diagnosis reflecting aging rather than a clinical syndrome and does not necessarily imply that the degenerative disc disease is the cause of the pain. To distinguish between nonverifiable radicular complaints without objective evidence of radiculopathy and unreliable vague complaints involving the extremity, evaluators should determine that the complaints are consistently and repetitively recognized in medical records and that they lie in the distribution of a single nerve root that the examiner can name. The diagnosis of “intervertebral disc herniation” cannot be made, and instead the “nonspecific chronic pain” diagnosis can be used. Nor can the diagnosis of alteration of motion segment integrity be used because the case lacks radiographically documented instability.

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