Biomimetic fetal rotation bioreactor for engineering bone tissues—Effect of cyclic strains on upregulation of osteogenic gene expression

2018 ◽  
Vol 12 (4) ◽  
pp. e2039-e2050 ◽  
Author(s):  
Akhilandeshwari Ravichandran ◽  
Feng Wen ◽  
Jing Lim ◽  
Mark Seow Khoon Chong ◽  
Jerry K.Y. Chan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

scholarly journals MicroRNA treatment modulates osteogenic differentiation potential of mesenchymal stem cells derived from human chorion and placenta

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kulisara Marupanthorn ◽  
Chairat Tantrawatpan ◽  
Pakpoom Kheolamai ◽  
Duangrat Tantikanlayaporn ◽  
Sirikul Manochantr
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Transcription Factor ◽  
Osteogenic Differentiation ◽  
Differentiation Potential ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

AbstractMesenchymal stem cells (MSCs) are important in regenerative medicine because of their potential for multi-differentiation. Bone marrow, chorion and placenta have all been suggested as potential sources for clinical application. However, the osteogenic differentiation potential of MSCs derived from chorion or placenta is not very efficient. Bone morphogenetic protein-2 (BMP-2) plays an important role in bone development. Its effect on osteogenic augmentation has been addressed in several studies. Recent studies have also shown a relationship between miRNAs and osteogenesis. We hypothesized that miRNAs targeted to Runt-related transcription factor 2 (Runx-2), a major transcription factor of osteogenesis, are responsible for regulating the differentiation of MSCs into osteoblasts. This study examines the effect of BMP-2 on the osteogenic differentiation of MSCs isolated from chorion and placenta in comparison to bone marrow-derived MSCs and investigates the role of miRNAs in the osteogenic differentiation of MSCs from these sources. MSCs were isolated from human bone marrow, chorion and placenta. The osteogenic differentiation potential after BMP-2 treatment was examined using ALP staining, ALP activity assay, and osteogenic gene expression. Candidate miRNAs were selected and their expression levels during osteoblastic differentiation were examined using real-time RT-PCR. The role of these miRNAs in osteogenesis was investigated by transfection with specific miRNA inhibitors. The level of osteogenic differentiation was monitored after anti-miRNA treatment. MSCs isolated from chorion and placenta exhibited self-renewal capacity and multi-lineage differentiation potential similar to MSCs isolated from bone marrow. BMP-2 treated MSCs showed higher ALP levels and osteogenic gene expression compared to untreated MSCs. All investigated miRNAs (miR-31, miR-106a and miR148) were consistently downregulated during the process of osteogenic differentiation. After treatment with miRNA inhibitors, ALP activity and osteogenic gene expression increased over the time of osteogenic differentiation. BMP-2 has a positive effect on osteogenic differentiation of chorion- and placenta-derived MSCs. The inhibition of specific miRNAs enhanced the osteogenic differentiation capacity of various MSCs in culture and this strategy might be used to promote bone regeneration. However, further in vivo experiments are required to assess the validity of this approach.

Exogenous micro-RNA and antagomir modulate osteogenic gene expression in tenocytes

2019 ◽  
Vol 378 (2) ◽  
pp. 119-123
Author(s):  
Michelle Xiao ◽  
Kag C. Iglinski-Benjamin ◽  
Orr Sharpe ◽  
William H. Robinson ◽  
Geoffrey D. Abrams
Keyword(s):  
Gene Expression ◽  
Micro Rna ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

scholarly journals Bone-specific overexpression of DMP1 influences osteogenic gene expression during endochondral and intramembranous ossification

2014 ◽  
Vol 55 (sup1) ◽  
pp. 121-124 ◽  
Author(s):  
Joshua D. Padovano ◽  
Amsaveni Ramachandran ◽  
Sara Bahmanyar ◽  
Sriram Ravindran ◽  
Anne George
Keyword(s):  
Gene Expression ◽  
Intramembranous Ossification ◽  
Osteogenic Gene Expression ◽  
Osteogenic Gene

Effects of nicotine in the presence and absence of vitamin E on morphology, viability and osteogenic gene expression in MG-63 osteoblast-like cells

2016 ◽  
Vol 27 (6) ◽  
Author(s):  
Maryam Torshabi ◽  
Zeinab Rezaei Esfahrood ◽  
Parisan Gholamin ◽  
Elahe Karami
Keyword(s):  
Gene Expression ◽  
Vitamin E ◽  
Bone Loss ◽  
Marker Genes ◽  
Proliferation And Differentiation ◽  
Osteogenic Gene Expression ◽  
Polymerase Chain ◽  
Presence And Absence ◽  
Osteoblast Markers ◽  
Osteogenic Gene

AbstractBackground:Evidence shows that oxidative stress induced by nicotine plays an important role in bone loss. Vitamin E with its antioxidative properties may be able to reverse the effects of nicotine on bone. This study aimed to assess the effects of nicotine in the presence and absence of vitamin E on morphology, viability and osteogenic gene expression in MG-63 (osteosarcoma) human osteoblast-like cells.Methods:We treated the cells with 5 mM nicotine. The viability and morphology of cells were evaluated respectively using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) and crystal violet assays. The effect of nicotine on osteogenic gene expression in MG-63 cells was assessed by real-time reverse-transcription polymerase chain reaction of osteoblast markers, namely, alkaline phosphatase, osteocalcin and bone sialoprotein.Results:The results revealed that survival and proliferation of MG-63 cells were suppressed following exposure to nicotine, and cytoplasm vacuolization occurred in the cells. Nicotine significantly down-regulated the expression of osteogenic marker genes. Such adverse effects on morphology, viability and osteogenic gene expression of MG-63 cells were reversed by vitamin E therapy.Conclusions:In conclusion, vitamin E supplementation may play a role in proliferation and differentiation of osteoblasts, and vitamin E can be considered as an anabolic agent to treat nicotine-induced bone loss.

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