scholarly journals Nanofibrous gelatine scaffolds integrated with nerve growth factor‐loaded alginate microspheres for brain tissue engineering

2017 ◽  
Vol 12 (2) ◽  
Author(s):  
Melda Büyüköz ◽  
Esra Erdal ◽  
Sacide Alsoy Altinkaya
Keyword(s):  
Tissue Engineering ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Brain Tissue ◽  
Nerve Growth

scholarly journals Neuroactive conducting scaffolds: nerve growth factor conjugation on active ester-functionalized polypyrrole

2008 ◽  
Vol 6 (38) ◽  
pp. 801-810 ◽  
Author(s):  
Jae Young Lee ◽  
Joo-Woon Lee ◽  
Christine E. Schmidt
Keyword(s):  
Tissue Engineering ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Photoelectron Spectroscopy ◽  
Electrical Potential ◽  
Biologically Active ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nerve Growth ◽  
Active Ester ◽  
Novel Scaffold

Electrically conductive and biologically active scaffolds are desirable for enhancing adhesion, proliferation and differentiation of a number of cell types such as neurons. Hence, the incorporation of neuroactive molecules into electroconductive polymers via a specific and stable method is essential for neuronal tissue engineering applications. Traditional conjugation approaches dramatically impair conductivities and/or stabilities of the scaffolds and ligands. In this study, we developed copolymers (PPy-NSE) of N -hydroxyl succinimidyl ester pyrrole and regular pyrrole, which can be immobilized with nerve growth factor (NGF) without significantly hindering electroconductivity. The presence of active ester groups was confirmed using reflectance infrared spectroscopy and X-ray photoelectron spectroscopy (XPS) from the copolymers prepared from different monomer compositions. We selected PPy-NSE 50 (polymerized from a 50 : 50 monomer ratio of pyrrole : pyrrole-NSE) for further modification with NGF because this copolymer retains good conductivity (approx. 8 S cm −1 ) and presents active ester groups for NGF immobilization. We tethered NGF on the PPy-NSE 50 surface, and found that PC12 cells extended neurites similarly to cells cultured in NGF-containing medium. XPS and enzyme-linked immunosorbent assay confirmed that NGF immobilized via the active ester on the PPy-NSE 50 film was stable for up to 5 days in phosphate-buffered saline solution. Also, application of an external electrical potential to NGF-immobilized PPy films did not cause a significant release of NGF nor reduce their neurotrophic activity. This novel scaffold, providing electroconductive and neurotrophic activities, has potential for neural applications, such as tissue engineering scaffolds and biosensors.

scholarly journals Reduced Nerve Growth Factor in Rett Syndrome Postmortem Brain Tissue

2000 ◽  
Vol 59 (10) ◽  
pp. 889-895 ◽  
Author(s):  
John D. Lipani ◽  
Meena B. Bhattacharjee ◽  
David M. Corey ◽  
Deborah A. Lee
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Brain Tissue ◽  
Rett Syndrome ◽  
Postmortem Brain ◽  
Nerve Growth ◽  
Postmortem Brain Tissue
Sign in / Sign up

Export Citation Format

Share Document