Enhancedin vivosurvival of Schwann cells by a synthetic oxygen carrier promotes sciatic nerve regeneration and functional recovery

2017 ◽  
Vol 12 (1) ◽  
pp. e177-e189 ◽  
Author(s):  
Teng Ma ◽  
Lei Zhu ◽  
Yafeng Yang ◽  
Xin Quan ◽  
Liangliang Huang ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Functional Recovery ◽  
Oxygen Carrier ◽  
Sciatic Nerve Regeneration

A synthetic oxygen carrier in fibrin matrices promotes sciatic nerve regeneration in rats

2013 ◽  
Vol 9 (7) ◽  
pp. 7248-7263 ◽  
Author(s):  
Y. Wang ◽  
F. Qi ◽  
S. Zhu ◽  
Z. Ye ◽  
T. Ma ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Oxygen Carrier ◽  
Sciatic Nerve Regeneration

scholarly journals Salidroside promotes sciatic nerve regeneration following combined application epimysium conduit and Schwann cells in rats

2020 ◽  
Vol 245 (6) ◽  
pp. 522-531
Author(s):  
Jiaqi Li ◽  
Yongguang Zhang ◽  
Zhimin Yang ◽  
Jingxian Zhang ◽  
Ren Lin ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Western Blot ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Motor Function ◽  
Nerve Conduit ◽  
Combined Application ◽  
Post Operation ◽  
Sciatic Nerve Regeneration

Schwann cell and nerve conduit are crucial for nerve regeneration and re-myelination after peripheral nerves injury. To investigate the effects of Salidroside on autogenous epimysium conduit mixed with BD matrigel and RSC96 Schwann cells on an animal model with 5 mm sciatic nerve defect injury in rat, motor function, muscle reinnervation, immunohistochemical staining, retrograded tracing and Western blot were used in this study. The results showed that Salidroside enhanced the compound effects of epimysium conduit mixed with BD matrigel and RSC96 Schwann cells to improve the sciatic functional index and the gastrocnemius muscle weight ratio, which were better than EPM group at 8 weeks and 12 weeks post operation. Immunofluorescence and Western blot results of P75NTR showed that Salidroside improved the sciatic nerve regeneration, and retrograded tracing of CTB-Alexa 488 also supported that Salidroside was better to promote CTB tracer transporting from the distal nerve defect to the ipsilateral dorsal root ganglion and ventral horn of L3-L5 spinal cord on post-operation 8 weeks and 12 weeks. Our results demonstrated that Salidroside improved the effect of autogenous epimysium conduit mixed with BD matrigel and RSC96 Schwann cells on sciatic nerve regeneration in our study. Impact statement Peripheral nerve injury and regeneration remain a major challenge. Although nerve conduit and Schwann cells have been used to study the nerve regeneration, our results demonstrated that Salidroside improved the regenerative effect in a rat model with sciatic nerve injury model, following a combined application of autogenous epimysium conduit mixed with Schwann cells. Different concentrations of Salidroside combining autogenous epimysium conduit and Schwann cells were applied to compare the epimysium conduit group and the epimysium conduit combining Schwann cells group. Based on the results of motor function and muscle reinnervation evaluation, as well as neuronal tracing and expression of P75NTR, our study for the first time suggests that Salidroside may improve the regeneration effect on the sciatic nerve following a combined application of epimysium conduit and RSC96 Schwann cells in rats.

scholarly journals In Vitro and In Vivo Effects of Nobiletin on DRG Neurite Elongation and Axon Growth after Sciatic Nerve Injury

2021 ◽  
Vol 18 (17) ◽  
pp. 8988
Author(s):  
Tae-Beom Seo ◽  
Yoon-A Jeon ◽  
Sang Suk Kim ◽  
Young Jae Lee
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Axon Growth ◽  
Sciatic Nerve Injury ◽  
Dependent Manner ◽  
Neurite Elongation ◽  
Sciatic Nerve Regeneration

Sciatic nerve injury (SNI) leads to sensory and motor dysfunctions. Nobiletin is a major component of polymethoxylated flavonoid extracted from citrus fruits. The role of nobiletin on sciatic nerve regeneration is still unclear. Thus, the purpose of this study was to investigate whether nobiletin increases DRG neurite elongation and regeneration-related protein expression after SNI. Cytotoxicity of nobiletin was measured in a concentration–dependent manner using the MTT assay. For an in vitro primary cell culture, the sciatic nerve on the middle thigh was crushed by holding twice with forceps. Dorsal root ganglion (DRG) and Schwann cells were cultured 3 days after SNI and harvested 36 h later and 3 days later, respectively. In order to evaluate specific regeneration-related markers and axon growth in the injured sciatic nerve, we applied immunofluorescence staining and Western blot techniques. Nobiletin increased cell viability in human neuroblastoma cells and inhibited cytotoxicity induced by exposure to H2O2. Mean neurite length of DRG neurons was significantly increased in the nobiletin group at a dose of 50 and 100 μM compared to those at other concentrations. GAP-43, a specific marker for axonal regeneration, was enhanced in injury preconditioned Schwann cells with nobiletin treatment and nobiletin significantly upregulated it in injured sciatic nerve at only 3 days post crush (dpc). In addition, nobiletin dramatically facilitated axonal regrowth via activation of the BDNF-ERK1/2 and AKT pathways. These results should provide evidence to distinguish more accurately the biochemical mechanisms regarding nobiletin-activated sciatic nerve regeneration.

Sign in / Sign up

Export Citation Format

Share Document