Chitosan promotes aquaporin formation and inhibits mucociliary differentiation of nasal epithelial cells through increased TGF-β1 production

2016 ◽  
Vol 11 (12) ◽  
pp. 3567-3575 ◽  
Author(s):  
Tsung-Wei Huang ◽  
Ching-Kuo Wei ◽  
Huang-Wei Su ◽  
Kai-Min Fang
Keyword(s):  
Epithelial Cells ◽  
Nasal Epithelial Cells ◽  
Mucociliary Differentiation ◽  
Tgf Β1

Interleukin-13 Alters Mucociliary Differentiation of Human Nasal Epithelial Cells

CHEST Journal ◽  
2003 ◽  
Vol 123 (3) ◽  
pp. 373S-374S ◽  
Author(s):  
Marie Skowron ◽  
Eric Perret ◽  
Francelyne Marano ◽  
Daniel Caput ◽  
Frédéric Tournier
Keyword(s):  
Epithelial Cells ◽  
Nasal Epithelial Cells ◽  
Interleukin 13 ◽  
Human Nasal Epithelial Cells ◽  
Mucociliary Differentiation

TGF-β1 downregulates CFTR expression and function in nasal polyps of non-CF patients

2005 ◽  
Vol 288 (1) ◽  
pp. L77-L83 ◽  
Author(s):  
Virginie Prulière-Escabasse ◽  
Pascale Fanen ◽  
Anne Catherine Dazy ◽  
Emmanuèle Lechapt-Zalcman ◽  
Dominique Rideau ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Protein Expression ◽  
Nasal Polyps ◽  
Nasal Polyposis ◽  
Cftr Gene ◽  
Nasal Epithelial Cells ◽  
Human Nasal Epithelial Cells ◽  
And Function ◽  
Tgf Β1

Nasal polyposis is a chronic inflammatory disease of the upper airways. It has been suggested that ion transports and CFTR expression could be modified in epithelial cells from nasal polyps of non-cystic fibrosis patients. We compared human nasal epithelial cells from nasal polyps (NP) with control nasal mucosa (CM). The level of CFTR mRNA was studied by Northern blot analysis and protein expression was studied by immunoprecipitation both ex vivo and in vitro in primary cultures of human nasal epithelial cells at the air-liquid interface. Ion transports were evaluated by short-circuit measurements in vitro. CFTR gene and protein expressions were significantly decreased in NP native tissues and in culture on day 4, when a global defect of ion transports was observed in NP cultures, but not in CM. We evaluated the effect of transforming growth factor (TGF)-β1 on CFTR expression and function in NP cultures on day 14 and showed, for the first time, that TGF-β1 was able to significantly downregulate the level of CFTR mRNA and cAMP-dependent current in NP cultures. Finally, we showed that the effects of TGF-β1 on ion transports could be reversed after 48-h removal of TGF-β1 in NP cultures. In conclusion, our data strongly suggest that chronic inflammation in nasal polyposis downregulates CFTR gene and protein expression.

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