scholarly journals Three-dimensional culture of single embryonic stem-derived neural/stem progenitor cells in fibrin hydrogels: neuronal network formation and matrix remodelling

2016 ◽  
Vol 11 (12) ◽  
pp. 3494-3507 ◽  
Author(s):  
Ana R. Bento ◽  
Pedro Quelhas ◽  
Maria J. Oliveira ◽  
Ana P. Pêgo ◽  
Isabel F. Amaral
Keyword(s):  
Progenitor Cells ◽  
Neuronal Network ◽  
Network Formation ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Neural Stem Progenitor Cells ◽  
Three Dimensional Culture

Effects of Three-Dimensional Culture and Growth Factors on the Chondrogenic Differentiation of Murine Embryonic Stem Cells

Stem Cells ◽  
2006 ◽  
Vol 24 (2) ◽  
pp. 284-291 ◽  
Author(s):  
Nathaniel S. Hwang ◽  
Myoung Sook Kim ◽  
Somponnat Sampattavanich ◽  
Jin Hyen Baek ◽  
Zijun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Embryonic Stem Cells ◽  
Chondrogenic Differentiation ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Three Dimensional Culture

scholarly journals Establishment of a Human Neuronal Network Assessment System by Using a Human Neuron/Astrocyte Co-Culture Derived from Fetal Neural Stem/Progenitor Cells

2015 ◽  
Vol 21 (1) ◽  
pp. 54-64 ◽  
Author(s):  
Kazuyuki Fukushima ◽  
Yuji Miura ◽  
Kohei Sawada ◽  
Kazuto Yamazaki ◽  
Masashi Ito
Keyword(s):  
Drug Development ◽  
Progenitor Cells ◽  
Neuronal Network ◽  
Assessment System ◽  
Assay System ◽  
Success Rates ◽  
Drug Candidates ◽  
Human Neurons ◽  
Neural Stem Progenitor Cells ◽  
Network Functions

Using human cell models mimicking the central nervous system (CNS) provides a better understanding of the human CNS, and it is a key strategy to improve success rates in CNS drug development. In the CNS, neurons function as networks in which astrocytes play important roles. Thus, an assessment system of neuronal network functions in a co-culture of human neurons and astrocytes has potential to accelerate CNS drug development. We previously demonstrated that human hippocampus-derived neural stem/progenitor cells (HIP-009 cells) were a novel tool to obtain human neurons and astrocytes in the same culture. In this study, we applied HIP-009 cells to a multielectrode array (MEA) system to detect neuronal signals as neuronal network functions. We observed spontaneous firings of HIP-009 neurons, and validated functional formation of neuronal networks pharmacologically. By using this assay system, we investigated effects of several reference compounds, including agonists and antagonists of glutamate and γ-aminobutyric acid receptors, and sodium, potassium, and calcium channels, on neuronal network functions using firing and burst numbers, and synchrony as readouts. These results indicate that the HIP-009/MEA assay system is applicable to the pharmacological assessment of drug candidates affecting synaptic functions for CNS drug development.

scholarly journals A Three-Dimensional Culture Method to Expand Limbal Stem/Progenitor Cells

2014 ◽  
Vol 20 (5) ◽  
pp. 393-400 ◽  
Author(s):  
Hua Mei ◽  
Sheyla González ◽  
Martin N. Nakatsu ◽  
Elfren Ray Baclagon ◽  
Vanda S. Lopes ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Three Dimensional ◽  
Culture Method ◽  
Three Dimensional Culture
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