Cartilage tissue engineering on macroporous scaffolds using human tooth germ stem cells

2015 ◽  
Vol 11 (3) ◽  
pp. 765-777 ◽  
Author(s):  
A. C. Calikoglu Koyuncu ◽  
G. Gurel Pekozer ◽  
M. Ramazanoglu ◽  
G. Torun Kose ◽  
V. Hasirci
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cartilage Tissue Engineering ◽  
Tooth Germ ◽  
Cartilage Tissue ◽  
Human Tooth ◽  
Human Tooth Germ

In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells

2014 ◽  
Vol 39 (1) ◽  
pp. 94-103 ◽  
Author(s):  
Ayşegül Doğan ◽  
Selami Demirci ◽  
Fikrettin Şahin
Keyword(s):  
Stem Cells ◽  
Tooth Germ ◽  
Human Tooth ◽  
In Vitro Differentiation ◽  
Human Tooth Germ

Influence of STRO-1 selection on osteogenic potential of human tooth germ derived mesenchymal stem cells

2017 ◽  
Vol 82 ◽  
pp. 293-301 ◽  
Author(s):  
Pinar Ercal ◽  
Gorke G. Pekozer ◽  
Osman Z. Gumru ◽  
Gamze T. Kose ◽  
Mustafa Ramazanoglu
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tooth Germ ◽  
Human Tooth ◽  
Osteogenic Potential ◽  
Human Tooth Germ

Spidroin Striped Micropattern Promotes Chondrogenic Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cells

2021 ◽  
Author(s):  
Anggraini Barlian ◽  
Dinda Hani’ah Arum Saputri ◽  
Adriel Hernando ◽  
Ekavianty Prajatelistia ◽  
Hutomo Tanoto
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Spider Silk ◽  
Cartilage Tissue Engineering ◽  
Wharton’S Jelly ◽  
Cartilage Tissue ◽  
Type Ii ◽  
Wharton's Jelly

Abstract Cartilage tissue engineering, particularly micropattern, can influence the biophysical properties of mesenchymal stem cells (MSCs) leading to chondrogenesis. In this research, human Wharton’s jelly MSCs (hWJ-MSCs) were grown on a striped micropattern containing spider silk protein (spidroin) from Argiope appensa. This research aims to direct hWJ-MSCs chondrogenesis using micropattern made of spidroin bioink as opposed to fibronectin that often used as the gold standard. Cells were cultured on striped micropattern of 500 µm and 1000 µm width sizes without chondrogenic differentiation medium for 21 days. The immunocytochemistry result showed that spidroin contains RGD sequences and facilitates cell adhesion via integrin β1. Chondrogenesis was observed through the expression of glycosaminoglycan, type II collagen, and SOX9. The result on glycosaminoglycan content proved that 1000 µm was the optimal width to support chondrogenesis. Spidroin micropattern induced significantly higher expression of SOX9 mRNA on day-21 and SOX9 protein was located inside the nucleus starting from day-7. COL2A1 mRNA of spidroin micropattern groups was downregulated on day-21 and collagen type II protein was detected starting from day-14. These results showed that spidroin micropattern enhances chondrogenic markers while maintains long-term upregulation of SOX9, and therefore has the potential as a new method for cartilage tissue engineering.

TRENDS AND CHALLENGES OF CARTILAGE TISSUE ENGINEERING

2009 ◽  
Vol 21 (03) ◽  
pp. 149-155 ◽  
Author(s):  
Hsu-Wei Fang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Growth Factors ◽  
Bone Cells ◽  
Cartilage Tissue Engineering ◽  
Bioactive Molecules ◽  
In Vivo Studies ◽  
Cartilage Tissue

Cartilage injuries may be caused by trauma, biomechanical imbalance, or degenerative changes of joint. Unfortunately, cartilage has limited capability to spontaneous repair once damaged and may lead to progressive damage and degeneration. Cartilage tissue-engineering techniques have emerged as the potential clinical strategies. An ideal tissue-engineering approach to cartilage repair should offer good integration into both the host cartilage and the subchondral bone. Cells, scaffolds, and growth factors make up the tissue engineering triad. One of the major challenges for cartilage tissue engineering is cell source and cell numbers. Due to the limitations of proliferation for mature chondrocytes, current studies have alternated to use stem cells as a potential source. In the recent years, a lot of novel biomaterials has been continuously developed and investigated in various in vitro and in vivo studies for cartilage tissue engineering. Moreover, stimulatory factors such as bioactive molecules have been explored to induce or enhance cartilage formation. Growth factors and other additives could be added into culture media in vitro, transferred into cells, or incorporated into scaffolds for in vivo delivery to promote cellular differentiation and tissue regeneration.Based on the current development of cartilage tissue engineering, there exist challenges to overcome. How to manipulate the interactions between cells, scaffold, and signals to achieve the moderation of implanted composite differentiate into moderate stem cells to differentiate into hyaline cartilage to perform the optimum physiological and biomechanical functions without negative side effects remains the target to pursue.

Sign in / Sign up

Export Citation Format

Share Document