Mesenchymal stem cells and ligand incorporation in biomimetic poly(ethylene glycol) hydrogels significantly improve insulin secretion from pancreatic islets

2014 ◽  
Vol 11 (3) ◽  
pp. 694-703 ◽  
Author(s):  
Tuğba Bal ◽  
Caner Nazli ◽  
Alparslan Okcu ◽  
Gökhan Duruksu ◽  
Erdal Karaöz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Insulin Secretion ◽  
Ethylene Glycol ◽  
Pancreatic Islets ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Structural engineering to control density, conformation, and bioactivity of the poly(ethylene glycol)-grafted poly(urethane urea) scaffolds

2018 ◽  
Vol 34 (2) ◽  
pp. 209-223
Author(s):  
Shideh Shaneh ◽  
Fatemeh Shokrolahi ◽  
Parvin Shokrollahi ◽  
Hamid Yeganeh ◽  
Hossein Omidian
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Structural Engineering ◽  
Cell Attachment ◽  
Poly Ethylene Glycol ◽  
Salt Leaching ◽  
The Matrix ◽  
Diphenyl Tetrazolium Bromide ◽  
Poly Ethylene

Poly(urethane urea) scaffolds were fabricated through combined salt leaching and solvent casting methods. The scaffolds were then functionalized via aminolysis with poly(ethylene glycol) (PEG- g-PUU). To compare its bioactivity, gelatin was also grafted onto the aminolyzed poly(urethane urea) surface (Gel- g-PUU). Chemical changes at the surface were then monitored using quantitative/qualitative methods. Grafting with both gelatin and poly(ethylene glycol) remarkably enhanced the wettability of poly(urethane urea). Proliferation of human adipose–derived mesenchymal stem cells on poly(urethane urea) and the modified poly(urethane urea)s was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. The cell experiment results showed that both the modified poly(urethane urea)s enhanced the attachment and proliferation of human adipose–derived mesenchymal stem cells compared to pure poly(urethane urea). Based on previous reports, while a supportive role is observed at adequate poly(ethylene glycol) graft densities, cell adhesion and proliferation are inhibited at very high grafting densities. To correlate the cell data to poly(ethylene glycol) conformations, the surface tension was measured. Data on human adipose–derived mesenchymal stem cells’ attachment/proliferation and contact angle/surface free energy together showed that the grafting density of poly(ethylene glycol) was regulated by optimizing aminolysis conditions, careful selection of poly(ethylene glycol)’s molecular weight, and bulk properties of the matrix poly(urethane urea). As a result, surface overcrowding and brush conformation of the poly(ethylene glycol) chains were avoided, and human adipose–derived mesenchymal stem cell attachment and proliferation occurred on the PEG- g-PUU scaffold at a comparable level to the Gel- g-PUU.

scholarly journals Poly(3-hydroxybutyrate)/poly(ethylene glycol) scaffolds with different microstructure: the effect on growth of mesenchymal stem cells

3 Biotech ◽  
2018 ◽  
Vol 8 (8) ◽  
Author(s):  
A. P. Bonartsev ◽  
I. I. Zharkova ◽  
V. V. Voinova ◽  
E. S. Kuznetsova ◽  
V. A. Zhuikov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Effect On Growth

scholarly journals Osteogenic differentiation of human mesenchymal stem cells on poly(ethylene glycol)-variant biomaterials

2009 ◽  
Vol 91A (4) ◽  
pp. 975-984 ◽  
Author(s):  
Tonye Briggs ◽  
Matthew D. Treiser ◽  
Paul F. Holmes ◽  
Joachim Kohn ◽  
Prabhas V. Moghe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals The effect of low-magnitude, high-frequency vibration on poly(ethylene glycol)-microencapsulated mesenchymal stem cells

2018 ◽  
Vol 9 ◽  
pp. 204173141880010 ◽  
Author(s):  
Sneha Mehta ◽  
Brooke McClarren ◽  
Ayesha Aijaz ◽  
Rabab Chalaby ◽  
Kimberly Cook-Chennault ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
High Frequency ◽  
Three Dimensional ◽  
Poly Ethylene Glycol ◽  
Natural Sources ◽  
High Frequency Vibration ◽  
Poly Ethylene ◽  
Low Magnitude

Low-magnitude, high-frequency vibration has stimulated osteogenesis in mesenchymal stem cells when these cells were cultured in certain types of three-dimensional environments. However, results of osteogenesis are conflicting with some reports showing no effect of vibration at all. A large number of vibration studies using three-dimensional scaffolds employ scaffolds derived from natural sources. Since these natural sources potentially have inherent biochemical and microarchitectural cues, we explored the effect of low-magnitude, high-frequency vibration at low, medium, and high accelerations when mesenchymal stem cells were encapsulated in poly(ethylene glycol) diacrylate microspheres. Low and medium accelerations enhanced osteogenesis in mesenchymal stem cells while high accelerations inhibited it. These studies demonstrate that the isolated effect of vibration alone induces osteogenesis.

3D Culture of Tonsil-Derived Mesenchymal Stem Cells in Poly(ethylene glycol)-Poly(l-alanine-co-l-phenyl alanine) Thermogel

2014 ◽  
Vol 3 (11) ◽  
pp. 1782-1791 ◽  
Author(s):  
Min Hee Park ◽  
Yeonsil Yu ◽  
Hyo Jung Moon ◽  
Du Young Ko ◽  
Han Su Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
3D Culture ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Phenyl Alanine
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