Maxillary sinus floor elevation with a tissue-engineered bone composite of deciduous tooth stem cells and calcium phosphate cement in goats

2014 ◽  
Vol 11 (1) ◽  
pp. 66-76 ◽  
Author(s):  
Wei Zhao ◽  
Jia-Yu Lu ◽  
Yong-Ming Hao ◽  
Chun-Hua Cao ◽  
De-Rong Zou
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Maxillary Sinus ◽  
Calcium Phosphate Cement ◽  
Deciduous Tooth ◽  
Sinus Floor Elevation ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Maxillary Sinus Floor Elevation

scholarly journals Evaluation of a new biphasic calcium phosphate for maxillary sinus floor elevation: Micro-CT and histomorphometrical analyses

2018 ◽  
Vol 29 (5) ◽  
pp. 488-498 ◽  
Author(s):  
Marco N. Helder ◽  
Fransisca A. S. van Esterik ◽  
Mardi D. Kwehandjaja ◽  
Christiaan M. ten Bruggenkate ◽  
Jenneke Klein-Nulend ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Maxillary Sinus ◽  
Biphasic Calcium Phosphate ◽  
Micro Ct ◽  
Sinus Floor Elevation ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Maxillary Sinus Floor Elevation

scholarly journals Effects of Platelet-rich Fibrin on Osteogenic Differentiation of Schneiderian Membrane Derived Mesenchymal Stem Cells and Bone Formation in Maxillary Sinus

2021 ◽  
Author(s):  
Yanmin Zhou ◽  
Jia Wang ◽  
Yue Sun ◽  
Yiping Liu ◽  
Jize Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Maxillary Sinus ◽  
Sinus Floor Elevation ◽  
Schneiderian Membrane ◽  
Maxillary Sinus Floor ◽  
Sinus Floor ◽  
Maxillary Sinus Floor Elevation

Abstract BackgroundThe existence of mesenchymal stem cells (MSCs) in Schneiderian membrane has not been determined. The aim of this study is to investigate whether there are MSCs in Schneiderian membrane, and the effect of platelet-rich fibrin (PRF) on osteogenic differentiation of these cells and on new bone formation in maxillary sinus after maxillary sinus floor elevation.MethodsSchneiderian membrane derived mesenchymal stem cells (SM-MSCs) were isolate from rabbit maxillary sinus. Cells were identified by flow cytometry and multipotential differentiation. Real-time cell analysis assay, fluorescence staining, transwell assay, and wound healing assay were used to determine the effects of PRF stimulation on cell proliferation and migration. The osteogenic differentiation ability of cells stimulated by PRF or osteoinductive medium (OM) was evaluated by alkaline phosphatase (ALP) staining, alizarin red staining, PCR and Western blot. Equivalent volume Bio-oss and the mixture of Bio-oss and PRF were used as bone graft materials for maxillary sinus floor elevation. Micro-CT, bone double-staining, HE staining, Masson staining, and toluidine blue staining were used to evaluate the osteogenic effect in 8 and 12 weeks after surgery.ResultsThe cell surface markers were positive for expression of CD90, CD105, and negative for expression of CD34, CD45. SM-MSCs had the ability of osteogenic, adipogenic and chondrogenic differentiation. PRF can stimulate proliferation, migration and osteogenic differentiation of SM-MSCs, which is achieved by up-regulating ERK 1/2 signaling pathway. PRF can accelerate the formation of new bone in maxillary sinus and increase the amount of new bone formation. ConclusionsMSCs exist in Schneiderian membrane, and PRF stimulation can promote cell proliferation, migration and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation can accelerate bone healing and increase the quantity and quality of new bone.

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