Time-lapse photographic study of neural tube closure defects caused by xylocaine in the chick

Teratology ◽  
1988 ◽  
Vol 37 (3) ◽  
pp. 263-269 ◽  
Author(s):  
H. Lee ◽  
K. T. Bush ◽  
R. G. Nagele
Keyword(s):  
Neural Tube ◽  
Time Lapse ◽  
Neural Tube Closure ◽  
Tube Closure ◽  
Neural Tube Closure Defects

scholarly journals Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure

2011 ◽  
Vol 195 (6) ◽  
pp. 1047-1060 ◽  
Author(s):  
Yoshifumi Yamaguchi ◽  
Naomi Shinotsuka ◽  
Keiko Nonomura ◽  
Kiwamu Takemoto ◽  
Keisuke Kuida ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Neural Tube ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Time Lapse ◽  
Neural Tube Closure ◽  
Caspase Activation ◽  
Fluorescent Reporter ◽  
Time Lapse Imaging ◽  
Tube Closure

Many cells die during development, tissue homeostasis, and disease. Dysregulation of apoptosis leads to cranial neural tube closure (NTC) defects like exencephaly, although the mechanism is unclear. Observing cells undergoing apoptosis in a living context could help elucidate their origin, behavior, and influence on surrounding tissues, but few tools are available for this purpose, especially in mammals. In this paper, we used insulator sequences to generate a transgenic mouse that stably expressed a genetically encoded fluorescence resonance energy transfer (FRET)–based fluorescent reporter for caspase activation and performed simultaneous time-lapse imaging of apoptosis and morphogenesis in living embryos. Live FRET imaging with a fast-scanning confocal microscope revealed that cells containing activated caspases showed typical and nontypical apoptotic behavior in a region-specific manner during NTC. Inhibiting caspase activation perturbed and delayed the smooth progression of cranial NTC, which might increase the risk of exencephaly. Our results suggest that caspase-mediated cell removal facilitates NTC completion within a limited developmental window.

DESMOPRESSIN FOR THE TREATMENT OF NOCTURNAL BEDWETTING IN PATIENTS WITH NEURAL TUBE CLOSURE DEFECTS

2004 ◽  
Vol 171 (4) ◽  
pp. 1656-1658 ◽  
Author(s):  
R. DEL GADO ◽  
G. ACETO ◽  
D. DEL GAIZO ◽  
G. DEL GADO ◽  
G. POLIDORI ◽  
...  
Keyword(s):  
Neural Tube ◽  
Neural Tube Closure ◽  
Tube Closure ◽  
Neural Tube Closure Defects

scholarly journals Heritability of cranium bifidum and spina bifida in the golden hamster

1979 ◽  
Vol 34 (2) ◽  
pp. 189-194 ◽  
Author(s):  
A. M. Moffa ◽  
J. A. White
Keyword(s):  
Environmental Factors ◽  
Spina Bifida ◽  
Neural Tube ◽  
Golden Hamster ◽  
Average Frequency ◽  
Neural Tube Closure ◽  
Cranium Bifidum ◽  
Tube Closure ◽  
Half Sibling ◽  
Neural Tube Closure Defects

SUMMARYThe heritability (h2) and frequency of the neural tube closure defects, cranium bifidum (CB) and spina bifida (SB), have been estimated for a population of 9-day-old hamster embryos through half-sibling analysis. The average frequency of the total affected embryos per litter is approximately 17% while the pooled estimate for h2 based on between sires and between dams within sires components was 4%. This value points to the importance of environmental factors in contributing to the variance in defect frequencies observed within this population.

scholarly journals Metabolite profiling of whole murine embryos reveals metabolic perturbations associated with maternal valproate-induced neural tube closure defects

2017 ◽  
Vol 109 (2) ◽  
pp. 106-119 ◽  
Author(s):  
Darya Akimova ◽  
Bogdan J. Wlodarczyk ◽  
Ying Lin ◽  
M. Elizabeth Ross ◽  
Richard H. Finnell ◽  
...  
Keyword(s):  
Neural Tube ◽  
Metabolite Profiling ◽  
Neural Tube Closure ◽  
Murine Embryos ◽  
Tube Closure ◽  
Neural Tube Closure Defects
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