The teratogenic effects of 6-mercaptopurine on chick embryos in ovo

Teratology ◽  
1975 ◽  
Vol 11 (2) ◽  
pp. 179-185 ◽  
Author(s):  
Randall B. Grubb ◽  
Ethel C. Montiegel
Keyword(s):  
Chick Embryos ◽  
In Ovo ◽  
Teratogenic Effects

Teratogenic Effects of Herpes Simplex, Vaccinia, Influenza - A (NWS), and Distemper Virus Infections on Early Chick Embryos.

1956 ◽  
Vol 92 (4) ◽  
pp. 675-682 ◽  
Author(s):  
H. D. Heath ◽  
H. H. Shear ◽  
D. T. Imagawa ◽  
M. H. Jones ◽  
J. M. Adams
Keyword(s):  
Herpes Simplex ◽  
Influenza A ◽  
Chick Embryos ◽  
Virus Infections ◽  
Teratogenic Effects

Early changes in limb buds of chick embryos after thalidomide treatment

Development ◽  
1966 ◽  
Vol 16 (2) ◽  
pp. 289-300
Author(s):  
A. Jurand
Keyword(s):  
Embryonic Development ◽  
Experimental Investigations ◽  
Experimental Production ◽  
Chick Embryos ◽  
Teratogenic Effects ◽  
Limb Buds ◽  
Experimental Animals ◽  
Almost All ◽  
Thalidomide Treatment

Since the first observations of hypoplastic and aplastic thalidomide deformities in infants (McBride, 1961; Lenz, 1962), the literature on this subject has grown to many hundreds of communications. Experimental investigations in almost all cases have been undertaken to show whether thalidomide and its metabolites have any teratogenic effects in experimental animals. Numerous review papers are available on this subject, e.g. Giroud, Tuchmann-Duplessis & Mercier-Parot (1962), Somers (1963), and Salzgeber & Wolff (1964). Chick embryos did not seem for some time to be suitable for experimental production of typical thalidomide deformities. However, Kemper (1962a, b), Yang, Yang & Liang (1962). Boylen, Home & Johnson (1963) and Leone (1963) have shown that thalidomide can produce a whole range of ectromelian deformities provided that it is introduced into the egg at a particular period of embryonic development.

scholarly journals Effect of in ovo feeding of L-glutamine + ISP to chick embryos

2019 ◽  
Vol 13 (4) ◽  
pp. 591
Author(s):  
João Paulo Ferreira Rufino ◽  
Frank George Guimarães Cruz ◽  
Valcely Da Rocha Costa ◽  
André Ferreira Silva ◽  
Pedro Alves de Oliveira Filho ◽  
...  
Keyword(s):  
Chick Embryos ◽  
In Ovo ◽  
In Ovo Feeding

scholarly journals A detailed description of an economical setup for electroporation of chick embryos in ovo

2013 ◽  
Vol 46 (9) ◽  
pp. 752-757 ◽  
Author(s):  
R.M. Borges ◽  
J.H. Horne ◽  
A. Melo ◽  
J.T. Vidal ◽  
F.M. Vieceli ◽  
...  
Keyword(s):  
Chick Embryos ◽  
In Ovo

scholarly journals Automated Segmentation and Object Classification of CT Images: Application toIn VivoMolecular Imaging of Avian Embryos

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Alexander Heidrich ◽  
Jana Schmidt ◽  
Johannes Zimmermann ◽  
Hans Peter Saluz
Keyword(s):  
Image Analysis ◽  
Image Segmentation ◽  
Bone Growth ◽  
Imaging Techniques ◽  
Long Bone ◽  
Automated Segmentation ◽  
Chick Embryos ◽  
In Ovo

Background. Although chick embryogenesis has been studied extensively, there has been growing interest in the investigation of skeletogenesis. In addition to improved poultry health and minimized economic loss, a greater understanding of skeletal abnormalities can also have implications for human medicine. Truein vivostudies require noninvasive imaging techniques such as high-resolution microCT. However, the manual analysis of acquired images is both time consuming and subjective.Methods. We have developed a system for automated image segmentation that entails object-based image analysis followed by the classification of the extracted image objects. For image segmentation, a rule set was developed using Definiens image analysis software. The classification engine was implemented using the WEKA machine learning tool.Results. Our system reduces analysis time and observer bias while maintaining high accuracy. Applying the system to the quantification of long bone growth has allowed us to present the first truein ovodata for bone length growth recorded in the same chick embryos.Conclusions. The procedures developed represent an innovative approach for the automated segmentation, classification, quantification, and visualization of microCT images. MicroCT offers the possibility of performing longitudinal studies and thereby provides unique insights into the morpho- and embryogenesis of live chick embryos.

Optogenetic regulation of leg movement in midstage chick embryos through peripheral nerve stimulation

2011 ◽  
Vol 106 (5) ◽  
pp. 2776-2782 ◽  
Author(s):  
Andrew A. Sharp ◽  
Sylvia Fromherz
Keyword(s):  
Nervous System ◽  
Neuronal Activity ◽  
Expression System ◽  
Continuous Illumination ◽  
Chick Embryos ◽  
Motor Axons ◽  
In Ovo ◽  
Full Extension ◽  
Sensorimotor Development ◽  
Spontaneous Movements

Numerous disorders that affect proper development, including the structure and function of the nervous system, are associated with altered embryonic movement. Ongoing challenges are to understand in detail how embryonic movement is generated and to understand better the connection between proper movement and normal nervous system function. Controlled manipulation of embryonic limb movement and neuronal activity to assess short- and long-term outcomes can be difficult. Optogenetics is a powerful new approach to modulate neuronal activity in vivo. In this study, we have used an optogenetics approach to activate peripheral motor axons and thus alter leg motility in the embryonic chick. We used electroporation of a transposon-based expression system to produce ChIEF, a channelrhodopsin-2 variant, in the lumbosacral spinal cord of chick embryos. The transposon-based system allows for stable incorporation of transgenes into the genomic DNA of recipient cells. ChIEF protein is detectable within 24 h of electroporation, largely membrane-localized, and found throughout embryonic development in both central and peripheral processes. The optical clarity of thin embryonic tissue allows detailed innervation patterns of ChIEF-containing motor axons to be visualized in the living embryo in ovo, and pulses of blue light delivered to the thigh can elicit stereotyped flexures of the leg when the embryo is at rest. Continuous illumination can disrupt full extension of the leg during spontaneous movements. Therefore, our results establish an optogenetics approach to alter normal peripheral axon function and to probe the role of movement and neuronal activity in sensorimotor development throughout embryogenesis.

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