Antigenotoxic properties of selenium compounds on potassium dichromate and hydrogen peroxide

2003 ◽  
Vol 23 (S2) ◽  
pp. 53-67 ◽  
Author(s):  
Eduardo Cemeli ◽  
Joanna Carder ◽  
Diana Anderson ◽  
Emma Guillamet ◽  
Mar�a Jos� Morillas ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Potassium Dichromate ◽  
Selenium Compounds

Preparing Diatoms for EM

2000 ◽  
Vol 8 (9) ◽  
pp. 36-37
Author(s):  
Greg Strout
Keyword(s):  
Electron Microscopy ◽  
Hydrogen Peroxide ◽  
Scanning Electron Microscopy ◽  
Diatomaceous Earth ◽  
Potassium Dichromate ◽  
Scanning Electron

Diatoms make for beautiful specimens for both transmission and scanning electron microscopy. As well, they are studied by many people, and there is always a need for good diatom preparations for EM.I find that the diatoms in diatomaceous earth are usually broken, and like to prepare my own from fresh specimens. The materials needed for this are a plankton net, some potassium dichromate, and 30% hydrogen peroxide. Once the diatoms are collected (plankton net tows from shoreline, or wherever), they can be cleaned using the chemicals.

scholarly journals Comparison of Landfill Leachate Treatment Efficiency Using the Advanced Oxidation Processes

2013 ◽  
Vol 39 (2) ◽  
pp. 107-115 ◽  
Author(s):  
Barbara Pieczykolan ◽  
Izabela Płonka ◽  
Krzysztof Barbusiński ◽  
Magdalena Amalio-Kosel
Keyword(s):  
Hydrogen Peroxide ◽  
Organic Compounds ◽  
Advanced Oxidation Processes ◽  
Potassium Dichromate ◽  
Advanced Oxidation ◽  
Fenton’S Reagent ◽  
Nitrogen And Phosphorus ◽  
Leachate Treatment ◽  
Fenton's Reagent ◽  
Oxidation Processes

Abstract Treatment of leachate from an exploited since 2004 landfill by using two methods of advanced oxidation processes was performed. Fenton’s reagent with two different doses of hydrogen peroxide and iron and UV/H2O2 process was applied. The removal efficiency of biochemically oxidizable organic compounds (BOD5), chemically oxidizable compounds using potassium dichromate (CODCr) and nutrient (nitrogen and phosphorus) was examined. Studies have shown that the greatest degree of organic compounds removal expressed as a BOD5 index and CODCr index were obtained when Fenton’s reagent with greater dose of hydrogen peroxide was used - efficiency was respectively 72.0% and 69.8%. Moreover, in this case there was observed an increase in the value of ratio of BOD5/CODCr in treated leachate in comparison with raw leachate. Application of Fenton’s reagent for leachate treatment also allowed for more effective removal of nutrients in comparison with the UV/H2O2 process.

scholarly journals Genotoxic and antigenotoxic properties of selenium compounds in thein vitromicronucleus assay with human whole blood lymphocytes and tk6 lymphoblastoid cells

2006 ◽  
Vol 6 ◽  
pp. 1202-1210 ◽  
Author(s):  
Eduard Cemeli ◽  
Ricard Marcos ◽  
Diana Anderson
Keyword(s):  
Whole Blood ◽  
Potassium Dichromate ◽  
Sodium Selenate ◽  
Protective Effects ◽  
Selenium Compounds ◽  
Blood Lymphocytes ◽  
Selenous Acid ◽  
Tk6 Cells ◽  
Lower Frequencies

Selenium is known to possess both genotoxic and antigenotoxic properties. In the present study, we have evaluated the genotoxicity and antigenotoxicity of three selenium compounds (sodium selenate, sodium selenite and selenous acid) by measuring in vitro micronucleus induction. Assays were conducted in whole blood lymphocytes and in the TK6 lymphoblastoid cell line, with and without co-treatment with potassium dichromate, a known genotoxic compound. In general, the compounds were more active in TK6 cells than they were in blood lymphocytes. Only 1 μM selenous acid increased the frequency of binucleated cells containing micronuclei (BNMN) in blood lymphocytes, while all three selenium compounds increased BNMN in TK6 cells. In addition, combinations of selenous acid and potassium dichromate resulted in lower frequencies of BNMN than potassium dichromate alone in blood lymphocytes, while combinations of sodium selenate and potassium dichromate produced lower frequencies of BNMN than potassium dichromate alone in TK6 cells. The concentrations of selenium compounds that were used, in combination with the medium components and the biological physiology of the whole blood lymphocytes and TK6 cells, could have affected the redox potential of the compounds, switching the chemicals from a pro-oxidant to antioxidant status and vice-versa. The lower activities of the compounds in blood lymphocytes may be due to the protective effects of blood components. The results indicate that the genotoxic and antigenotoxic properties of selenium compounds are highly dependent upon the conditions under which they are evaluated.

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