A plea to DNA barcode type specimens: An example from Micropholis (Sapotaceae)

Taxon ◽  
2021 ◽  
Author(s):  
Dayana Sánchez‐C. ◽  
James E. Richardson ◽  
Michelle Hart ◽  
Julieth Serrano ◽  
Dairon Cárdenas ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Type Specimens

A new species of the genus Parapentacentrus Shiraki, 1930 from Yunnan, China (Orthoptera: Gryllidae: Itarinae)

Zootaxa ◽  
2021 ◽  
Vol 5032 (1) ◽  
pp. 143-146
Author(s):  
YI-JIAO LIU ◽  
ZHU-QING HE
Keyword(s):  
New Species ◽  
Male Genitalia ◽  
Dna Barcode ◽  
Coi Gene ◽  
East China ◽  
Anal Plate ◽  
Type Specimens ◽  
Normal University ◽  
One New Species ◽  
A New Species

The genus Parapentacentrus Shiraki, 1930 includes two species with long wings. In this paper, we report one new species, P. brevipennis He sp. nov., from Jinping, Yunnan, China. The new species have short forewings and hindwings, and have differences in the shape of supra-anal plate and male genitalia. DNA Barcode (COI gene) of this new species are provided. The type specimens are deposited in Museum of Biology, East China Normal University (ECNU).  

scholarly journals Three new species of the segmented spider genus Qiongthela (Mesothelae, Liphistiidae) from Hainan Island, China

ZooKeys ◽  
2021 ◽  
Vol 1009 ◽  
pp. 123-138
Author(s):  
Li Yu ◽  
Fengxiang Liu ◽  
Zengtao Zhang ◽  
Daiqin Li ◽  
Xin Xu
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Hainan Island ◽  
Morphological Characters ◽  
Type Specimens ◽  
Oxidase Subunit ◽  
Three New Species

We report three new species of the segmented trapdoor spider genus Qiongthela Xu & Kuntner, 2015 collected from Hainan Island, China based on morphological characters: Q. dongfangsp. nov. (♂♀), Q. nankaisp. nov. (♂♀), Q. yalinsp. nov. (♂♀). We also provide the GenBank accession codes of the DNA barcode gene, cytochrome c oxidase subunit I (COI), of the type specimens of all three new species to aid future identification.

scholarly journals Calibrating the taxonomy of a megadiverse insect family: 3000 DNA barcodes from geometrid type specimens (Lepidoptera, Geometridae)

Genome ◽  
2016 ◽  
Vol 59 (9) ◽  
pp. 671-684 ◽  
Author(s):  
Axel Hausmann ◽  
Scott E. Miller ◽  
Jeremy D. Holloway ◽  
Jeremy R. deWaard ◽  
David Pollock ◽  
...  
Keyword(s):  
18Th Century ◽  
Dna Barcode ◽  
Data Systems ◽  
Dna Barcodes ◽  
Reference Library ◽  
Type Specimens ◽  
Voucher Specimen ◽  
Tropical Regions ◽  
Taxonomic Assignments ◽  
Insect Family

It is essential that any DNA barcode reference library be based upon correctly identified specimens. The Barcode of Life Data Systems (BOLD) requires information such as images, geo-referencing, and details on the museum holding the voucher specimen for each barcode record to aid recognition of potential misidentifications. Nevertheless, there are misidentifications and incomplete identifications (e.g., to a genus or family) on BOLD, mainly for species from tropical regions. Unfortunately, experts are often unavailable to correct taxonomic assignments due to time constraints and the lack of specialists for many groups and regions. However, considerable progress could be made if barcode records were available for all type specimens. As a result of recent improvements in analytical protocols, it is now possible to recover barcode sequences from museum specimens that date to the start of taxonomic work in the 18th century. The present study discusses success in the recovery of DNA barcode sequences from 2805 type specimens of geometrid moths which represent 1965 species, corresponding to about 9% of the 23 000 described species in this family worldwide and including 1875 taxa represented by name-bearing types. Sequencing success was high (73% of specimens), even for specimens that were more than a century old. Several case studies are discussed to show the efficiency, reliability, and sustainability of this approach.

Sorting out Lalos: description of new species and additional taxonomic data on megophryid frogs from northern Indochina (genus Leptolalax, Megophryidae, Anura)

Zootaxa ◽  
2011 ◽  
Vol 3147 (1) ◽  
pp. 1 ◽  
Author(s):  
ANNEMARIE OHLER ◽  
KATHARINA C. WOLLENBERG ◽  
STÉPHANE GROSJEAN ◽  
RALF HENDRIX ◽  
MIGUEL VENCES ◽  
...  
Keyword(s):  
New Species ◽  
Conservation Status ◽  
Dna Barcode ◽  
Type Specimen ◽  
Molecular Study ◽  
Molecular Data ◽  
Nominal Species ◽  
Type Specimens ◽  
Species Identity ◽  
Rdna Sequences

Frogs in the subgenus Lalos of the genus Leptolalax (Megophryidae) are highly diversified in continental Asia and consist of about 17 nominal species. These frogs are small, inconspicuous, and of high superficial morphological similarity.        We here formulate a hypothesis of phylogenetic relationships and assess the amount of genetic variation among genealogical lineages on the basis of 536bp of mitochondrial 16S rDNA sequences. Combining molecular data with a study of morpho-logy, morphometric divergence and geographical proximity, we tested hypotheses of species identity. We (1) used character-based and morphometric analyses to assign the onymophoronts (type specimens) of species in Lalos available to us to respectively one of the main clades, in order to propose the best potential correct taxonomic and nomenclatural allocation for the individuals included in the molecular study, and (2) tried to also assign the historical museum specimens to these molecular taxonomic units and to reclassify them whenever necessary.        We also used the molecular data to match tadpoles with adults and provide tadpole descriptions for species the larvae of which were previously unknown. Specimens, that could neither be allocated to a molecularly characterised species (on the basis of their DNA “barcode”) nor to a morphologically defined species named on the basis of a type specimen, are described here as new species. Based on this integrative set of data and analyses we describe two new species, Leptolalax eos n. sp. and Leptolalax nyx n. sp., we resurrect Leptolalax minimus, and reassess the distribution of the species studied. We propose changes in the Red List status of L. pelodytoides and L. ventripunctatus and suggest a conservation status for the new species described herein.

scholarly journals Diatom DNA barcodes for forensic discrimination of drowning incidents

2020 ◽  
Vol 367 (17) ◽  
Author(s):  
Mengyan Liu ◽  
Yi Zhao ◽  
Yuzhe Sun ◽  
Ping Wu ◽  
Shiliang Zhou ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Dna Barcode ◽  
Dna Barcodes ◽  
Type Specimens ◽  
Internal Organs ◽  
Sequencing Platform ◽  
Next Generation Sequencing Platform ◽  
Urgent Task ◽  
Dna Metabarcoding ◽  
Generation Sequencing

ABSTRACT The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.In this study we tested 23 pairs of primers, including two new primer pairs, Baci18S (V4 of 18S) and BacirbcL (central region of rbcL), for amplifying fragments of 16S/18S, 23S/28S, COI, ITS and rbcL. A total of five pairs of primers performed satisfactory for diatoms. We used three of them, 18S605 (V2 + V3 of 18S), Baci18S and BacirbcL, to barcode four water samples using next generation sequencing platform. The results showed that these primers worked well for NGS metabarcoding of diatoms. We suggest that 18S605, Baci18S and BacirbcL be barcodes of diatoms and the corresponding primer pairs be used. Considering a quite high proportion of sequences deposited in GenBank were mislabeled, the most urgent task for DNA barcoding of diatoms is to create standard sequences using correctly identified specimens, ideally type specimens.

Taxonomic revision of the genus Nychiodes Lederer, 1853 (Geometridae: Ennominae: Boarmiini) with description of three new species—an integrative approach

Zootaxa ◽  
2020 ◽  
Vol 4812 (1) ◽  
pp. 1-61
Author(s):  
DOMINIC WANKE ◽  
AXEL HAUSMANN ◽  
LARS KROGMANN ◽  
GERGELY PETRÁNYI ◽  
HOSSEIN RAJAEI
Keyword(s):  
New Species ◽  
Dna Barcode ◽  
Taxonomic Revision ◽  
Integrative Approach ◽  
Wing Pattern ◽  
Type Specimens ◽  
Distribution Maps ◽  
Taxonomic Approach ◽  
Three New Species ◽  
A New Species

The non-European taxa of the genus Nychiodes Lederer, 1853 are revised. Type specimens of all described species and a large series of about 800 additional specimens were morphologically examined. More than 400 genitalia preparations were made and analyzed along with distributional and DNA barcode data. As a result of our integrative taxonomic approach, Nychiodes waltheri saerdabica Wehrli, 1938 syn. nov., is synonymized with N. waltheri Wagner, 1919; N. palaestinensis libanotica Zerny, 1933 syn. nov. is synonymized with N. palaestinensis Wagner, 1919 and the synonymy of N. persuavis Wehrli, 1929 syn. rev. with N. palaestinensis is confirmed; N. admirabila safidaria Wiltshire, 1943 syn. nov. is synonymized with N. admirabila Brandt, 1938; N. agatcha Brandt, 1938 syn. nov., N. subvirida disjuncta Wehrli, 1941 syn. nov. and N. subvirida taftana Brandt, 1941 syn. nov. are synonymized with N. subvirida Brandt, 1938. Also, N. variabila variabila Brandt, 1938 syn. nov., N. variabila opulenta Brandt, 1941 syn. nov., N. divergaria elbursica Wehrli, 1937 syn. nov., N. divergaria fallax Wehrli, 1939 syn. nov. and N. divergaria achtyca Wehrli, 1939 syn. nov. are synonymized with N. divergaria Staudinger, 1892. Nychiodes convergata sp. nov. from Israel, N. mirzayansi sp. nov. from the Iran and N. eberti sp. nov. from Turkey are described. Lecto- and paralectotypes are designated for N. palaestinensis, N. antiquaria, N. divergaria. Furthermore, N. antiquaria is reported as a new species for Pakistan, N. rayatica is reported as a new species for Iran and the hypothetical occurrence of N. amygdalaria in Iran is confirmed. Additionally, N. tyttha needs to be excluded from the genus. Wing pattern, male and female genitalia and diagnostic characters of all examined species are illustrated and distribution maps are provided. Illustrated keys based on genitalia, as well as a complete checklist of the genus is given here. 

scholarly journals A revision of the genera and species of the Neotropical family Mesembrinellidae (Diptera: Oestroidea)

Zootaxa ◽  
2019 ◽  
Vol 4659 (1) ◽  
pp. 1-146 ◽  
Author(s):  
TERRY L. WHITWORTH ◽  
SOHATH YUSSEFF-VANEGAS
Keyword(s):  
Costa Rica ◽  
French Guiana ◽  
Dna Barcode ◽  
South American ◽  
Neighbor Joining ◽  
Type Specimens ◽  
Extant Species ◽  
The Family ◽  
Species Groups ◽  
First Time

The Neotropical family Mesembrinellidae is revised. A total of 53 valid, extant species are included in the family, including 15 described as new and 38 redescribed based on study of type and non-type material and of the literature. A total of 18 primary types were examined. An additional ca. 2300 specimens, belonging to 47 species, were studied in detail, including dissection and photographic documentation of terminalia, with many females illustrated for the first time. Keys to subfamilies, genera, species-groups and species are provided. Type specimens of six species housed in South American institutions could not be obtained for study, i.e., M. bequaerti Séguy, 1925 and the five recently described species M. andina (Wolff et al., 2014), M. carvalhoi (Wolff et al., 2013b), M. cordillera (Wolff & Ramos-Pastrana in Wolff et al., 2017), M. obscura (Wolff in Wolff et al., 2017) and Laneella patriciae (Wolff, 2013). We accept the synonymy, proposed by previous authors, of Eumesembrinella Townsend, 1931 with Mesembrinella Giglio-Tos, 1893. In addition, we synonymize the genera Albuquerquea Mello, 1967, Giovanella Bonatto in Bonatto & Marinoni, 2005, Henriquella Bonatto in Bonatto & Marinoni, 2005, Huascaromusca Townsend, 1918 and Thompsoniella Guimarães, 1977 with Mesembrinella Giglio-Tos, 1893, synn. nov., retaining three valid genera in the family: Laneella Mello, 1967, Mesembrinella and Souzalopesiella Guimarães, 1977. Laneella nigripes Guimarães, 1977 and Mesembrinella bellardiana Aldrich, 1922 are fixed as the type species of the genera Laneella Mello, 1967 and Mesembrinella Giglio-Tos, 1893, respectively, under Article 70.3 of the ICZN Code. We separate Mesembrinella into the following species-groups: M. latifrons (Mello, 1967), M. spicata Aldrich, 1925, M. bolivar (Bonatto in Bonatto & Marinoni, 2005), M. aeneiventris (Wiedemann, 1830), M. bicolor (Fabricius, 1805), and M. anomala (Guimarães, 1977). The following 15 new species are described: Laneella fusconitida Whitworth, sp. nov. from Costa Rica, Ecuador and Venezuela, Laneella fuscosquamata Whitworth, sp. nov. from Guatemala and Mexico, Laneella purpurea Whitworth, sp. nov. from Costa Rica, Mesembrinella bullata Whitworth, sp. nov. from Bolivia, Mesembrinella chantryi Whitworth, sp. nov. from French Guiana and Brazil, Mesembrinella epandrioaurantia Whitworth, sp. nov. from Venezuela, Mesembrinella guaramacalensis Whitworth, sp. nov. from Venezuela, Mesembrinella longicercus Whitworth, sp. nov. from Bolivia, Mesembrinella mexicana Whitworth, sp. nov. from Mexico, Mesembrinella nigrocoerulea Whitworth, sp. nov. from Costa Rica, Ecuador and Venezuela, Mesembrinella serrata Whitworth, sp. nov. from Peru, Mesembrinella velasquezae Whitworth, sp. nov. from Venezuela, Mesembrinella violacea Whitworth, sp. nov. from Costa Rica, Mesembrinella woodorum Whitworth, sp. nov. from Ecuador, and Mesembrinella zurquiensis Whitworth, sp. nov. from Costa Rica. Mesembrinella abaca Hall, 1948 is proposed as a junior synonym of Mesembrinella socors (Walker, 1861), syn. nov. Lectotypes are designated for Dexia randa Walker, 1849 (now Mesembrinella) and Mesembrinella pictipennis Aldrich, 1922. We analyze the most extensive DNA-barcode dataset for Mesembrinellidae to date, encompassing the three genera considered valid and including 188 sequences (178 new) from 35 species, with data for 23 species provided for the first time. The topology of the resulting Neighbor-Joining tree is mostly congruent with morphology; however, some species show considerable genetic variation that is not reflected by morphology. Finally, we include a corrigendum to the recent Zootaxa paper on Nearctic Calliphora Robineau-Desvoidy (Diptera: Calliphoridae) by Tantawi et al.

Three new species of the primitively segmented spiders from Mt Yuelu, Hunan, China (Mesothelae, Liphistiidae)

Zootaxa ◽  
2019 ◽  
Vol 4691 (2) ◽  
pp. 139-152
Author(s):  
DENGQING LI ◽  
HAO LIU ◽  
XIN XU
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Morphological Characters ◽  
Molecular Data ◽  
Genetic Distances ◽  
Hunan Province ◽  
Type Specimens ◽  
Three New Species

We diagnose and describe two Songthela and one Vinathela species of the primitively segmented spiders, Liphistiidae, as new to science based on morphological characters and molecular data: Songthela pyriformis sp. nov. (male, female), Songthela shuyuan sp. nov. (male, female), Vinathela nenglianggu sp. nov. (male, female), which were collected at Mt Yuelu in Changsha, Hunan Province, China. To facilitate future identification of our species, we also provide the GenBank accession numbers of the DNA barcode gene, cytochrome c oxidase subunit I (COI) for all type specimens and the evidence of genetic distances based on COI for three new species. 

scholarly journals Four new species of the primitively segmented spider genus Qiongthela from Hainan Island, China (Mesothelae, Liphistiidae)

ZooKeys ◽  
2020 ◽  
Vol 911 ◽  
pp. 51-66 ◽  
Author(s):  
Li Yu ◽  
Fengxiang Liu ◽  
Zengtao Zhang ◽  
Yan Wang ◽  
Daiqin Li ◽  
...  
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Barcode ◽  
Hainan Island ◽  
Morphological Characters ◽  
Type Specimens ◽  
Southern Vietnam ◽  
Oxidase Subunit

The primitively segmented spider genus Qiongthela Xu & Kuntner, 2015 consists of seven species that are distributed in Hainan Island, China and southern Vietnam. Of the seven species, five are known from Hainan Island. In this study, four more Qiongthela species collected from Hainan Island are diagnosed and described as new to science based on morphological characters: Q. baotingsp. nov. (♂♀), Q. qiongzhongsp. nov. (♂♀), Q. sanyasp. nov. (♂♀), Q. yinggezuisp. nov. (♂♀). To facilitate future identification, the GenBank accession codes of the DNA barcode gene, cytochrome c oxidase subunit I (COI), for all the type specimens are also provided.

Sign in / Sign up

Export Citation Format

Share Document