In vivo characterization of two cell types in the rat globus pallidus which have opposite responses to dopamine receptor stimulation: Comparison of electrophysiological properties and responses to apomorphine, dizocilpine, and ketamine anesthesia

Synapse ◽  
1995 ◽  
Vol 20 (4) ◽  
pp. 338-350 ◽  
Author(s):  
Mark D. Kelland ◽  
Robert P. Soltis ◽  
Lisa A. Anderson ◽  
Debra A. Bergstrom ◽  
Judith R. Walters
Keyword(s):  
Dopamine Receptor ◽  
Globus Pallidus ◽  
Cell Types ◽  
Receptor Stimulation ◽  
Electrophysiological Properties ◽  
Ketamine Anesthesia ◽  
Responses To Dopamine ◽  
In Vivo Characterization

scholarly journals In Vivo Characterization of Endogenous Cardiovascular Extracellular Vesicles in Larval and Adult Zebrafish

2021 ◽  
Author(s):  
Aaron Scott ◽  
Lorena Sueiro Ballesteros ◽  
Marston Bradshaw ◽  
Chisato Tsuji ◽  
Ann Power ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Cell Types ◽  
Adult Zebrafish ◽  
Cell Type ◽  
Zebrafish Model ◽  
Cell Type Specific ◽  
In Vivo Characterization ◽  
Different Cell Types

Objective: Extracellular vesicles (EVs) facilitate molecular transport across extracellular space, allowing local and systemic signaling during homeostasis and in disease. Extensive studies have described functional roles for EV populations, including during cardiovascular disease, but the in vivo characterization of endogenously produced EVs is still in its infancy. Because of their genetic tractability and live imaging amenability, zebrafish represent an ideal but under-used model to investigate endogenous EVs. We aimed to establish a transgenic zebrafish model to allow the in vivo identification, tracking, and extraction of endogenous EVs produced by different cell types. Approach and Results: Using a membrane-tethered fluorophore reporter system, we show that EVs can be fluorescently labeled in larval and adult zebrafish and demonstrate that multiple cell types including endothelial cells and cardiomyocytes actively produce EVs in vivo. Cell-type specific EVs can be tracked by high spatiotemporal resolution light-sheet live imaging and modified flow cytometry methods allow these EVs to be further evaluated. Additionally, cryo electron microscopy reveals the full morphological diversity of larval and adult EVs. Importantly, we demonstrate the utility of this model by showing that different cell types exchange EVs in the adult heart and that ischemic injury models dynamically alter EV production. Conclusions: We describe a powerful in vivo zebrafish model for the investigation of endogenous EVs in all aspects of cardiovascular biology and pathology. A cell membrane fluorophore labeling approach allows cell-type specific tracing of EV origin without bias toward the expression of individual protein markers and will allow detailed future examination of their function.

scholarly journals In vivo characterization of the connectivity and subcomponents of the human globus pallidus

NeuroImage ◽  
2015 ◽  
Vol 120 ◽  
pp. 382-393 ◽  
Author(s):  
Patrick Beukema ◽  
Fang-Cheng Yeh ◽  
Timothy Verstynen
Keyword(s):  
Globus Pallidus ◽  
In Vivo Characterization

In Vivo Characterization of Cardiac Tissue Defects Associated With Pdgfrα Gene Deletion In the Patch Mouse

1999 ◽  
Vol 5 (S2) ◽  
pp. 1114-1115
Author(s):  
T. Thielen ◽  
L. Terracio ◽  
T.K. Borg ◽  
R.L. Price
Keyword(s):  
Cardiac Tissue ◽  
Active Form ◽  
Cell Types ◽  
Β Receptor ◽  
A Chain ◽  
In Vivo Characterization ◽  
Spontaneous Mutants ◽  
Tissue Defects

The active form of platelet derived growth factor (PDGF) is a polypeptide dimer of which there are three isoforms: PDGF-AA, PDGF-BB, and PDGF-AB. Furthermore, two types of PDGF receptors also exist: PDGFRα and PDGFRβ. The β-receptor binds only the PDGF-B chain with high affinity, while the αreceptor can bind either PDGF-A or -B chains. Both PDGF ligands and their receptors have been linked to such cellular responses as mitogenisis, cell migration, chemotaxis, adhesion and differentiation in a variety of cell types.Several studies have reported fetal cardiac abnormalities associated with disruption of PDGF and its receptors. In spontaneous mutants lacking the PDGF α-receptor (Patchmice), fetal hearts are generally dilated and exhibit valve and outflow tract defects. Similar defects are observed in normal embryos injected with antibodies to block the functioning of the PDGF-A chain. Disruption of PDGF-B function results in ventricular and atrial dilation, reduction of the ventricular wall, and hyper-trabeculation.

In Vivo Characterization of Muscarinic Receptor Subtypes That Mediate Vasodilatation in Patients With Essential Hypertension

Hypertension ◽  
1995 ◽  
Vol 26 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Tobias A. Bruning ◽  
Peter C. Chang ◽  
Maarten G.C. Hendriks ◽  
Pieter Vermeij ◽  
Martin Pfaffendorf ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Muscarinic Receptor ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
In Vivo Characterization

In vitro and in vivo characterization of graphene oxide coated porcine bone granules

Carbon ◽  
2016 ◽  
Vol 103 ◽  
pp. 291-298 ◽  
Author(s):  
Valeria Ettorre ◽  
Patrizia De Marco ◽  
Susi Zara ◽  
Vittoria Perrotti ◽  
Antonio Scarano ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
In Vivo Characterization
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