Subsurface cisterns in ?-motoneurons of the rat and cat: Immunohistochemical detection with antibodies against connexin32

Synapse ◽  
1991 ◽  
Vol 8 (2) ◽  
pp. 119-136 ◽  
Author(s):  
T. Yamamoto ◽  
E. L. Hertzberg ◽  
J. I. Nagy
Keyword(s):  
Immunohistochemical Detection ◽  
Subsurface Cisterns

Immunohistochemical detection of glycoprotein hormone alpha subunit in somatoprolactinic and pure somatotroph adenomas

1998 ◽  
Vol 21 (7) ◽  
pp. 434-440 ◽  
Author(s):  
M. C. Vantyghem ◽  
C. Cortet ◽  
C. Bauters ◽  
M. H. Gevaert ◽  
D. Dewailly ◽  
...  
Keyword(s):  
Alpha Subunit ◽  
Immunohistochemical Detection ◽  
Glycoprotein Hormone

Immunohistochemical Detection of Cancer-Testis Antigen PRAME

2021 ◽  
pp. 106689692110120
Author(s):  
Cecilia Lezcano ◽  
Annette M. Müller ◽  
Denise Frosina ◽  
Enmily Hernandez ◽  
Jerica A. Geronimo ◽  
...  
Keyword(s):  
Protein Expression ◽  
Molecular Data ◽  
Immunohistochemical Detection ◽  
Normal Tissues ◽  
Variable Extent ◽  
Formalin Fixed Paraffin Embedded ◽  
Myxoid Liposarcomas ◽  
Fetal Ovary ◽  
Ct Antigens ◽  
Cancer Testis

Cancer-testis (CT) antigens were identified by their ability to elicit T- or B-cell immune responses in the autologous host. They are typically expressed in a wide variety of neoplasms and in normal adult tissues are restricted to testicular germ cells. PReferentially expressed Antigen of Melanoma (PRAME) is a member of the family of nonclassical CT antigens being expressed in a few other normal tissues besides testis. Interestingly, knowledge about the protein expression of many CT antigens is still incomplete due to the limited availability of reagents for their immunohistochemical detection. Here, we tested several commercially available serological reagents and identified a monoclonal antibody suitable for the immunohistochemical detection of PRAME in formalin-fixed paraffin-embedded specimens. We also tested a wide array of normal and neoplastic tissues. PRAME protein expression in normal tissues is congruent with original molecular data being present in the testis, and at low levels in the endometrium, adrenal cortex, and adult as well as fetal ovary. In tumors, there is diffuse PRAME immunoreactivity in most metastatic melanomas, myxoid liposarcomas, and synovial sarcomas. Other neoplasms such as seminomas and carcinomas of various origins including endometrial, serous ovarian, mammary ductal, lung, and renal showed an intermediate proportion of cases and variable extent of tumor cells positive for PRAME protein expression. As seen with other CT antigens, hepatocellular and colorectal carcinoma, Leydig cell tumors, mesothelioma, and leiomyosarcoma are poor expressers of PRAME.

Enhancement of immunohistochemical detection of HIV-1 p24 antigen in brain by tyramide signal amplification

1997 ◽  
Vol 67 (1) ◽  
pp. 103-112 ◽  
Author(s):  
Pádraig M Strappe ◽  
Ting Huei Wang ◽  
Chris Anne McKenzie ◽  
Suzanne Lowrie ◽  
Peter Simmonds ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Immunohistochemical Detection ◽  
Tyramide Signal Amplification ◽  
P24 Antigen ◽  
Hiv 1
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