Presence of brain spectrin in dendrites of mammalian brain: Technical factors involved in immunocytochemical detection

Synapse ◽  
1988 ◽  
Vol 2 (3) ◽  
pp. 329-333 ◽  
Author(s):  
Gwen O. Ivy ◽  
Peter Seubert ◽  
Michel Baudry ◽  
Gary Lynch
Keyword(s):  
Mammalian Brain ◽  
Immunocytochemical Detection ◽  
Technical Factors ◽  
Brain Spectrin

scholarly journals Antigen preservation tests for immunocytochemical detection of cytoskeletal proteins: influence of aldehyde fixatives.

1989 ◽  
Vol 37 (5) ◽  
pp. 675-681 ◽  
Author(s):  
B M Riederer
Keyword(s):  
Monoclonal Antibodies ◽  
Brain Tissue ◽  
Polyclonal Antibody ◽  
Cytoskeletal Proteins ◽  
Immunocytochemical Localization ◽  
Neurofilament Proteins ◽  
Tissue Samples ◽  
Immunocytochemical Detection ◽  
Immunochemical Detection ◽  
Brain Spectrin

The effects of aldehyde fixatives on immunochemical detection of cytoskeletal proteins were demonstrated by applying several quantitative assays to evaluate antigen conservation. Immunologically detectable brain spectrin (240/235) was measured by dot-immunobinding and quantitative immunodot assay using a polyclonal antibody. Paraformaldehyde fixation led to a 43-66% reduction in brain spectrin (240/235) immunodetection, and increasing glutaraldehyde concentrations decreased the immunological detection even more. Quantitative cryosection immunoassay and immunocytochemical localization confirmed the aldehyde sensitivity of brain spectrin (240/235). Brain spectrin (240/235) immunoreactivity decreased with increasing protein crosslinking and was dependent on glutaraldehyde concentration and post-fixation period. The assays were also used to test for conservation of antigenicity of neurofilament proteins by two monoclonal antibodies. Neurofilament detection was abolished in brain tissue after aldehyde fixation. The described methods allow screening within 24 hr of many fixation conditions by use of purified proteins as well as brain tissue samples, and allow an estimate of fixative influence on the conservation of protein antigenicity.

Absence of brain spectrin(240/235) in dendrites of mammalian brain

1989 ◽  
Vol 23 (1-2) ◽  
pp. 19-24 ◽  
Author(s):  
Ian S. Zagon ◽  
Steven R. Goodman
Keyword(s):  
Mammalian Brain ◽  
Brain Spectrin

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

Opiate-Inhibited Adenylate Cyclase in Mammalian Brain Membranes

1986 ◽  
Author(s):  
Steven R. Childers ◽  
Peter Nijssen ◽  
Pauline Nadeau ◽  
Page Buckhannan ◽  
Phi-Van Le ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Mammalian Brain ◽  
Brain Membranes

The effect of technical factors on hue distribution in elastographic images: a phantom study

2008 ◽  
Vol 29 (S 1) ◽  
Author(s):  
SM Dudea ◽  
C Botar-Jid ◽  
D Dumitriu ◽  
A Ciurea ◽  
A Chiorean ◽  
...  
Keyword(s):  
Phantom Study ◽  
Technical Factors

NeuN As a Neuronal Nuclear Antigen and Neuron Differentiation Marker

Acta Naturae ◽  
2015 ◽  
Vol 7 (2) ◽  
pp. 42-47 ◽  
Author(s):  
V. V. Gusel’nikova ◽  
D. E. Korzhevskiy
Keyword(s):  
Experimental Data ◽  
Intracellular Localization ◽  
Nuclear Antigen ◽  
Structure And Properties ◽  
Immunocytochemical Detection ◽  
Perinuclear Cytoplasm ◽  
Diagnosis Of Cancer ◽  
Morphological Diagnosis ◽  
The Central Nervous System ◽  
Differentiation Marker

The NeuN protein is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. Monoclonal antibodies to the NeuN protein have been actively used in the immunohistochemical research of neuronal differentiation to assess the functional state of neurons in norm and pathology for more than 20 years. Recently, NeuN antibodies have begun to be applied in the differential morphological diagnosis of cancer. However, the structure of the protein, which can be revealed by antibodies to NeuN, remained unknown until recently, and the functions of the protein are still not fully clear. In the present mini-review, data on NeuN accumulated so far are summarized and analyzed. Data on the structure and properties of the protein, its isoforms, intracellular localization, and hypothesized functions are reported. The application field of immunocytochemical detection of NeuN in scientific and clinical studies, as well as the difficulties in the interpretation of the obtained experimental data and their possible causes, is described in details.

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