scholarly journals Altered NMDA receptor function in primary cultures of hippocampal neurons from mice lacking theHomer2gene

Synapse ◽  
2015 ◽  
Vol 70 (1) ◽  
pp. 33-39 ◽  
Author(s):  
C. Thetford Smothers ◽  
Karen K. Szumlinski ◽  
Paul F. Worley ◽  
John J. Woodward
Keyword(s):  
Nmda Receptor ◽  
Hippocampal Neurons ◽  
Primary Cultures ◽  
Receptor Function ◽  
Nmda Receptor Function

Survival and synaptogenesis of hippocampal neurons without NMDA receptor function in culture

1998 ◽  
Vol 10 (6) ◽  
pp. 2192-2198 ◽  
Author(s):  
Shigeo Okabe ◽  
Carlos Vicario-Abejón ◽  
Menahem Segal ◽  
Ronald D. G. McKay
Keyword(s):  
Nmda Receptor ◽  
Hippocampal Neurons ◽  
Receptor Function ◽  
Nmda Receptor Function

scholarly journals GPI-1046 Increases Presenilin-1 Expression and Restores NMDA Channel Activity

2010 ◽  
Vol 37 (4) ◽  
pp. 457-467 ◽  
Author(s):  
Joseph P. Steiner ◽  
Kathryn B. Payne ◽  
Christopher Drummond Main ◽  
Sabrina D'Alfonso ◽  
Kirsten X. Jacobsen ◽  
...  
Keyword(s):  
Nmda Receptor ◽  
Synaptic Transmission ◽  
Brain Slices ◽  
Synaptic Activity ◽  
Presenilin 1 ◽  
Receptor Function ◽  
Channel Function ◽  
Nmda Channel ◽  
Nmda Receptor Function ◽  
6 Hydroxydopamine

Background:Previously we showed that 6-hydroxydopamine lesions of the substantia nigra eliminate corticostriatal LTP and that the neuroimmunolophilin ligand (NIL), GPI-1046, restores LTP.Methods:We used cDNA microarrays to determine what mRNAs may be over- or under-expressed in response to lesioning and/or GPI-1046 treatment. Patch clamp recordings were performed to investigate changes in NMDA channel function before and after treatments.Results:We found that 51 gene products were differentially expressed. Among these we found that GPI-1046 treatment up-regulated presenilin-1 (PS-1) mRNA abundance. This finding was confirmed using QPCR. PS-1 protein was also shown to be over-expressed in the striatum of lesioned/GPI-1046-treated rats. As PS-1 has been implicated in controlling NMDA-receptor function and LTP is reduced by lesioning we assayed NMDA mediated synaptic activity in striatal brain slices. The lesion-induced reduction of dopaminergic innervation was accompanied by the near complete loss of NDMA receptor-mediated synaptic transmission between the cortex and striatum. GPI-1046 treatment of the lesioned rats restored NMDA-mediated synaptic transmission but not the dopaminergic innervation. Restoration of NDMA channel function was apparently specific as the sodium channel current density was also reduced due to lesioning but GPI-1046 did not reverse this effect. We also found that restoration of NMDA receptor function was also not associated with either an increase in NMDA receptor mRNA or protein expression.Conclusion:As it has been previously shown that PS-1 is critical for normal NMDA receptor function, our data suggest that the improvement of excitatory neurotransmission occurs through the GPI-1046-induced up-regulation of PS-1.

scholarly journals Control of NMDA Receptor Function by the NR2 Subunit Amino-Terminal Domain

2009 ◽  
Vol 29 (39) ◽  
pp. 12045-12058 ◽  
Author(s):  
H. Yuan ◽  
K. B. Hansen ◽  
K. M. Vance ◽  
K. K. Ogden ◽  
S. F. Traynelis
Keyword(s):  
Nmda Receptor ◽  
Receptor Function ◽  
Amino Terminal ◽  
Terminal Domain ◽  
Nmda Receptor Function ◽  
Amino Terminal Domain
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