Prenatal GABAB1 and GABAB2 receptors: Cellular and subcellular organelle localization in early fetal rat cortical neurons

Synapse ◽  
2006 ◽  
Vol 60 (8) ◽  
pp. 557-566 ◽  
Author(s):  
S.P. Li ◽  
H.Y. Lee ◽  
M.S. Park ◽  
J.Y. Bahk ◽  
B.C. Chung ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Subcellular Organelle ◽  
Fetal Rat ◽  
Rat Cortical Neurons

Laminin 1 Attenuates β-Amyloid Peptide Aβ(1-40) Neurotoxicity of Cultured Fetal Rat Cortical Neurons

2002 ◽  
Vol 73 (2) ◽  
pp. 742-749 ◽  
Author(s):  
Béatrice Drouet ◽  
Martine Pinçon Raymond ◽  
Jean Chambaz ◽  
Thierry Pillot
Keyword(s):  
Cortical Neurons ◽  
Amyloid Peptide ◽  
Fetal Rat ◽  
Rat Cortical Neurons ◽  
Β Amyloid ◽  
Β Amyloid Peptide ◽  
Laminin 1

Neurotrophic activity of honokiol on the cultures of fetal rat cortical neurons

2002 ◽  
Vol 12 (8) ◽  
pp. 1163-1166 ◽  
Author(s):  
Yoshiyasu Fukuyama ◽  
Kousuke Nakade ◽  
Yuka Minoshima ◽  
Ritsuko Yokoyama ◽  
Haifeng Zhai ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Neurotrophic Activity ◽  
Fetal Rat ◽  
Rat Cortical Neurons

scholarly journals α2-Macroglobulin Attenuates β-Amyloid Peptide 1-40 Fibril Formation and Associated Neurotoxicity of Cultured Fetal Rat Cortical Neurons

2002 ◽  
Vol 70 (3) ◽  
pp. 1182-1188 ◽  
Author(s):  
Yansheng Du ◽  
Kelly R. Bales ◽  
Richard C. Dodel ◽  
Xiaodong Liu ◽  
Michele A. Glinn ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Fibril Formation ◽  
Amyloid Peptide ◽  
Fetal Rat ◽  
Α2 Macroglobulin ◽  
Rat Cortical Neurons ◽  
Β Amyloid ◽  
Β Amyloid Peptide

scholarly journals A Modified Technique for Culturing Primary Fetal Rat Cortical Neurons

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Sui-Yi Xu ◽  
Yong-Min Wu ◽  
Zhong Ji ◽  
Xiao-Ya Gao ◽  
Su-Yue Pan
Keyword(s):  
Cortical Neurons ◽  
Dark Field ◽  
Complete Medium ◽  
Sprague Dawley ◽  
Modified Technique ◽  
Fetal Rat ◽  
Rat Cortical Neurons ◽  
Sequential Digestion ◽  
Medium Change

The study explored a modified primary culture system for fetal rat cortical neurons. Day E18 embryos from pregnant Sprague Dawley rats were microdissected under a stereoscope. To minimize enzymatic damage to the cultured neurons, we applied a sequential digestion protocol using papain and Dnase I. The resulting sifted cell suspension was seeded at a density of 50,000 cells per cm2onto 0.1 mg/mL L-PLL-covered vessels. After a four-hour incubation in high-glucose Dulbecco’s Modified Eagle’s Medium (HG-DMEM) to allow the neurons to adhere, the media was changed to neurobasal medium that was refreshed by changing half of the volume after three days followed by a complete medium change every week. The cells displayed progressively robust neurite extension, and nonneuronal-like cells could barely be detected by five daysin vitro(DIV); cell growth was still substantial at 14 DIV. Neurons were identified byβ-tubulin III immunofluorescence, and neuronal purity within the cultures was assessed at over 95% by both flow cytometry and by dark-field counting ofβ-tubulin III-positive cells. These results suggest that the protocol was successful and that the high purity of neurons in this system could be used as the basis for generating various cell models of neurological disease.

The Protective Effects of Jatrorrhizine on β-Amyloid (25-35)-Induced Neurotoxicity in Rat Cortical Neurons

2013 ◽  
Vol 11 (8) ◽  
pp. 1030-1037 ◽  
Author(s):  
Tao Luo ◽  
Wei Jiang ◽  
Yan Kong ◽  
Sheng Li ◽  
Feng He ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Protective Effects ◽  
Rat Cortical Neurons ◽  
Β Amyloid

scholarly journals Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Thu T. Duong ◽  
James Lim ◽  
Vidyullatha Vasireddy ◽  
Tyler Papp ◽  
Hung Nguyen ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Transgene Expression ◽  
Fluorescent Protein ◽  
Ex Vivo ◽  
Cell Types ◽  
Egfp Expression ◽  
Cell Type ◽  
Egfp Gene ◽  
Rat Cortical Neurons

Recombinant adeno-associated virus (rAAV), produced from a nonpathogenic parvovirus, has become an increasing popular vector for gene therapy applications in human clinical trials. However, transduction and transgene expression of rAAVs can differ acrossin vitroand ex vivo cellular transduction strategies. This study compared 11 rAAV serotypes, carrying one reporter transgene cassette containing a cytomegalovirus immediate-early enhancer (eCMV) and chicken beta actin (CBA) promoter driving the expression of an enhanced green-fluorescent protein (eGFP) gene, which was transduced into four different cell types: human iPSC, iPSC-derived RPE, iPSC-derived cortical, and dissociated embryonic day 18 rat cortical neurons. Each cell type was exposed to three multiplicity of infections (MOI: 1E4, 1E5, and 1E6 vg/cell). After 24, 48, 72, and 96 h posttransduction, GFP-expressing cells were examined and compared across dosage, time, and cell type. Retinal pigmented epithelium showed highest AAV-eGFP expression and iPSC cortical the lowest. At an MOI of 1E6 vg/cell, all serotypes show measurable levels of AAV-eGFP expression; moreover, AAV7m8 and AAV6 perform best across MOI and cell type. We conclude that serotype tropism is not only capsid dependent but also cell type plays a significant role in transgene expression dynamics.

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