Postsynaptic mechanism may contribute to inhibitory acetylcholine effect on GABAergic synaptic transmission in hippocampal cell cultures

Synapse ◽  
2001 ◽  
Vol 41 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Maksim V. Storozhuk ◽  
Igor V. Melnick ◽  
Platon G. Kostyuk ◽  
Pavel V. Belan
Keyword(s):  
Synaptic Transmission ◽  
Cell Cultures ◽  
Hippocampal Cell ◽  
Postsynaptic Mechanism ◽  
Gabaergic Synaptic Transmission

Post-tetanic depression of GABAergic synaptic transmission in rat hippocampal cell cultures

2002 ◽  
Vol 323 (1) ◽  
pp. 5-8 ◽  
Author(s):  
Maksim V. Storozhuk ◽  
Svetlana Y. Ivanova ◽  
Tatyana A. Pivneva ◽  
Igor V. Melnick ◽  
Galina G. Skibo ◽  
...  
Keyword(s):  
Synaptic Transmission ◽  
Cell Cultures ◽  
Hippocampal Cell ◽  
Tetanic Depression ◽  
Gabaergic Synaptic Transmission

Possible role of mitochondria in posttetanic potentiation of GABAergic synaptic transmission in rat neocortical cell cultures

Synapse ◽  
2005 ◽  
Vol 58 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Maksim V. Storozhuk ◽  
Svetlana Y. Ivanova ◽  
Pavel M. Balaban ◽  
Platon G. Kostyuk
Keyword(s):  
Synaptic Transmission ◽  
Cell Cultures ◽  
Posttetanic Potentiation ◽  
Gabaergic Synaptic Transmission

scholarly journals Gephyrin regulates GABAergic and glutamatergic synaptic transmission in hippocampal cell cultures.

2011 ◽  
Vol 286 (35) ◽  
pp. 31062.1-31062
Author(s):  
Zeynep Kasap Varley ◽  
Rocco Pizzarelli ◽  
Roberta Antonelli ◽  
Stefka H. Stancheva ◽  
Matthias Kneussel ◽  
...  
Keyword(s):  
Synaptic Transmission ◽  
Cell Cultures ◽  
Hippocampal Cell ◽  
Glutamatergic Synaptic Transmission

Homeostatic plasticity of GABA-ergic synaptic transmission in rat hippocampal cell cultures

2004 ◽  
Vol 36 (5-6) ◽  
pp. 385-390
Author(s):  
M. V. Storozhuk ◽  
S. Yu. Ivanova ◽  
P. G. Kostyuk
Keyword(s):  
Synaptic Transmission ◽  
Cell Cultures ◽  
Homeostatic Plasticity ◽  
Hippocampal Cell

scholarly journals Gephyrin Regulates GABAergic and Glutamatergic Synaptic Transmission in Hippocampal Cell Cultures

2011 ◽  
Vol 286 (23) ◽  
pp. 20942-20951 ◽  
Author(s):  
Zeynep Kasap Varley ◽  
Rocco Pizzarelli ◽  
Roberta Antonelli ◽  
Stefka H. Stancheva ◽  
Matthias Kneussel ◽  
...  
Keyword(s):  
Synaptic Transmission ◽  
Cell Cultures ◽  
Hippocampal Cell ◽  
Glutamatergic Synaptic Transmission

scholarly journals Mu-opioids suppress GABAergic synaptic transmission onto orbitofrontal cortex pyramidal neurons with subregional selectivity

2020 ◽  
Author(s):  
Benjamin K. Lau ◽  
Brittany P. Ambrose ◽  
Catherine S. Thomas ◽  
Min Qiao ◽  
Stephanie L. Borgland
Keyword(s):  
Synaptic Transmission ◽  
Opioid Receptor ◽  
Orbitofrontal Cortex ◽  
Pyramidal Neurons ◽  
Mu Opioid Receptor ◽  
Mu Opioid ◽  
Inhibitory Synaptic Transmission ◽  
Receptor Coupling ◽  
Mu Opioids ◽  
Gabaergic Synaptic Transmission

AbstractThe orbitofrontal cortex (OFC) plays a critical role in evaluating outcomes in a changing environment. Administering opioids to the OFC can alter the hedonic reaction to food rewards and increase their consumption in a subregion specific manner. However, it is unknown how mu-opioid signalling influences synaptic transmission in the OFC. Thus, we investigated the cellular actions of mu-opioids within distinct subregions of the OFC. Using in-vitro patch clamp electrophysiology in brain slices containing the OFC, we found that the mu-opioid agonist, DAMGO produced a concentration-dependant inhibition of GABAergic synaptic transmission onto medial OFC (mOFC), but not lateral OFC (lOFC) neurons. This effect was mediated by presynaptic mu-opioid receptor activation of local parvalbumin (PV+)-expressing interneurons. The DAMGO-induced suppression of inhibition was long-lasting and not reversed upon washout of DAMGO, or by application of the mu-opioid receptor antagonist, CTAP, suggesting an inhibitory long-term depression (iLTD) induced by an exogenous mu-opioid. We show that LTD at inhibitory synapses is dependent on downstream cAMP/PKA signaling, which differs between the mOFC and lOFC. Finally, we demonstrate that endogenous opioid release triggered via moderate physiological stimulation can induce LTD. Taken together, these results suggest that presynaptic mu-opioid stimulation of local PV+ interneurons induces a long-lasting suppression of GABAergic synaptic transmission, which depends on subregional differences in mu-opioid receptor coupling to the downstream cAMP/PKA intracellular cascade. These findings provide mechanistic insight into the opposing functional effects produced by mu-opioids within the OFC.Significance StatementConsidering that both the OFC and the opioid system regulate reward, motivation, and food intake; understanding the role of opioid signaling within the OFC is fundamental for a mechanistic understanding of the sequelae for several psychiatric disorders. This study makes several novel observations. First, mu-opioids induce a long-lasting suppression of inhibitory synaptic transmission onto OFC pyramidal neurons in a regionally selective manner. Secondly, mu-opioids recruit PV+ inputs to suppress inhibitory synaptic transmission in the mOFC. Thirdly, the regional selectivity of mu-opioid action of endogenous opioids is due to the efficacy of mu-opioid receptor coupling to the downstream cAMP/PKA intracellular cascades. These experiments are the first to reveal a cellular mechanism of opioid action within the OFC.

scholarly journals The C-terminal of CASY-1/Calsyntenin regulates GABAergic synaptic transmission at the Caenorhabditis elegans neuromuscular junction

PLoS Genetics ◽  
2018 ◽  
Vol 14 (3) ◽  
pp. e1007263 ◽  
Author(s):  
Shruti Thapliyal ◽  
Amruta Vasudevan ◽  
Yongming Dong ◽  
Jihong Bai ◽  
Sandhya P. Koushika ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Neuromuscular Junction ◽  
Synaptic Transmission ◽  
Gabaergic Synaptic Transmission ◽  
Casy 1
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