Effects of acute ?binge? cocaine on preprodynorphin, preproenkephalin, proopiomelanocortin, and corticotropin-releasing hormone receptor mRNA levels in the striatum and hypothalamic-pituitary-adrenal axis of mu-opioid receptor knockout mice

Synapse ◽  
2002 ◽  
Vol 45 (4) ◽  
pp. 220-229 ◽  
Author(s):  
Yan Zhou ◽  
Rudolph Spangler ◽  
Stefan D. Schlussman ◽  
Vadim P. Yuferov ◽  
Ichiro Sora ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Knockout Mice ◽  
Hormone Receptor ◽  
Mu Opioid Receptor ◽  
Mrna Levels ◽  
Corticotropin Releasing Hormone ◽  
Mu Opioid ◽  
Hypothalamic Pituitary Adrenal ◽  
Receptor Mrna ◽  
Pituitary Adrenal Axis

Cocaine modulates mu-opioid receptor mRNA but not c-fos mRNA levels in primary cortical astrocytes

2002 ◽  
Vol 58 (3) ◽  
pp. 285-288 ◽  
Author(s):  
Eugene D Festa ◽  
Christine Cecala ◽  
Vanya Quinones-Jenab ◽  
Shirzad Jenab
Keyword(s):  
Opioid Receptor ◽  
Mu Opioid Receptor ◽  
Mrna Levels ◽  
Mu Opioid ◽  
Receptor Mrna ◽  
Cortical Astrocytes

Chronic opioid antagonist administration upregulates mu opioid receptor binding without altering mu opioid receptor mRNA levels

1995 ◽  
Vol 33 (2) ◽  
pp. 351-355 ◽  
Author(s):  
Ellen M. Unterwald ◽  
Joshua M. Rubenfeld ◽  
Yasuo Imai ◽  
Jia-Bei Wang ◽  
George R. Uhl ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Receptor Binding ◽  
Mu Opioid Receptor ◽  
Opioid Antagonist ◽  
Mrna Levels ◽  
Mu Opioid ◽  
Receptor Mrna ◽  
Opioid Receptor Binding

Acute “binge” cocaine increases mu-opioid receptor mRNA levels in areas of the rat mesolimbic mesocortical dopamine system

1999 ◽  
Vol 48 (1) ◽  
pp. 109-112 ◽  
Author(s):  
Vadim Yuferov ◽  
Yan Zhou ◽  
Rudolph Spangler ◽  
Christopher E Maggos ◽  
Ann Ho ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Mu Opioid Receptor ◽  
Mrna Levels ◽  
Dopamine System ◽  
Mu Opioid ◽  
Receptor Mrna

Regulation of Mu Opioid Receptor mRNA Levels by Activation of Protein Kinase C in Human SH-SY5Y Neuroblastoma Cells 

1997 ◽  
Vol 87 (5) ◽  
pp. 1127-1138 ◽  
Author(s):  
Elizabeth K. Gies ◽  
Dawn M. Peters ◽  
Carol R. Gelb ◽  
Kathleen M. Knag ◽  
Robert A. Peterfreund
Keyword(s):  
Protein Synthesis ◽  
Neuropathic Pain ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Opioid Receptor ◽  
Mu Opioid Receptor ◽  
Mrna Levels ◽  
Mu Opioid ◽  
Receptor Mrna ◽  
Kinase C

Background The mu opioid receptor (MuOR) is a member of the superfamily of G protein-coupled receptors that mediates the analgesic actions of endogenous opioid peptides and the narcotic alkaloid derivatives of morphine. Activation and translocation of protein kinase C (PKC) by N-methyl-D-aspartate receptor stimulation correlates with resistance to opioid drugs in experimental states of neuropathic pain, but the cellular mechanisms of resistance have not been identified. One possibility is that PKC activation regulates MuOR mRNA expression and thus the ability to generate functional receptors. Using a human neuroblastoma cell line, the authors tested the hypothesis that phorbol ester activation of PKC regulates MuOR mRNA levels. Methods SH-SY5Y cells were maintained in a continuous monolayer culture and treated with phorbol esters or other agents before extraction of total cellular RNA. Slot-blot hybridization was used to measure the level of MuOR mRNA using 32P-labeled MuOR cDNA probes under high-stringency conditions. Autoradiograms were analyzed by scanning and densitometry. Results MuOR mRNA levels decreased in a dose- and time-dependent manner after tetradecanoyl phorbol acetate (TPA) was administered to activate PKC. The nadir, a level of approximately 50% of control, was at 2-8 h, followed by gradual recovery. The actions of TPA were blocked by pretreatment with the selective PKC inhibitor bisindolylmaleimide, but not by inhibition of protein synthesis with cycloheximide or anisomycin. The combination of TPA treatment and transcription inhibition with actinomycin D was associated with a transient increase in MuOR mRNA. Conclusions Mu opioid receptor mRNA levels are regulated by activation of PKC in a neuronal model. Protein kinase C effects which decrease MuOR mRNA levels appear largely independent of new protein synthesis, and cytotoxicity does not account for the findings. Plasticity of MuOR gene expression may contribute to variations in clinical responses to opioid analgesics in clinical states such as neuropathic pain.

Mu opioid receptor mRNA levels following chronic naltrexone administration

1994 ◽  
Vol 54 (1) ◽  
pp. 307-308 ◽  
Author(s):  
E.M. Unterwald ◽  
J.M. Rubenfeld ◽  
R. Spangler ◽  
Y. Imai ◽  
J.B. Wang ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Mu Opioid Receptor ◽  
Mrna Levels ◽  
Mu Opioid ◽  
Receptor Mrna

MU OPIOID RECEPTOR mRNA LEVELS FOLLOWING CHRONIC ADMINISTRATION OF OPIOID LIGANDS

Analgesia ◽  
1995 ◽  
Vol 1 (4) ◽  
pp. 805-808 ◽  
Author(s):  
Ellen M. Unterwald ◽  
Joshua M. Rubenfeld ◽  
Jason Kreuter ◽  
Mary Jeanne Kreek
Keyword(s):  
Opioid Receptor ◽  
Chronic Administration ◽  
Mu Opioid Receptor ◽  
Mrna Levels ◽  
Mu Opioid ◽  
Opioid Ligands ◽  
Receptor Mrna
Sign in / Sign up

Export Citation Format

Share Document