Rapid method for permanent slide preparation of colonies in soft agar cultures

1986 ◽  
Vol 4 (5) ◽  
pp. 368-374 ◽  
Author(s):  
Jazbieh Moezzi ◽  
Francis Ali-Osman ◽  
Martin J. Murphy
Keyword(s):  
Rapid Method ◽  
Soft Agar ◽  
Slide Preparation ◽  
Permanent Slide

Microscopic colonial morphology of an enterococcal L form

1968 ◽  
Vol 14 (6) ◽  
pp. 746-747
Author(s):  
Robert J. Anderson
Keyword(s):  
Direct Observation ◽  
Streptococcus Faecalis ◽  
Slide Preparation ◽  
Solid Media ◽  
Colonial Morphology ◽  
Permanent Slide

A possible reversion cycle from the L colony to the parent coccus in Streptococcus faecalis var. zymogenes on solid media is postulated. The peripheral large bodies of the L colony appeared to give rise to mycelial-like structures which extended away from the L colony and terminated in fragmenting cocci. This phenomenon was observed on a permanent slide preparation only and not from direct observation of growing organisms.

Ultrastructure of two neoplasms in culture compared with original biopsies

1984 ◽  
Vol 42 ◽  
pp. 50-51
Author(s):  
Joseph M. Harb ◽  
James T. Casper ◽  
Vlcki Piaskowski
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Biopsy Specimen ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Human Tumor ◽  
Soft Agar ◽  
Human Tumor Cells ◽  
Morphological Distinction ◽  
Tumor Types

The application of tissue culture and the newer methodologies of direct cloning and colony formation of human tumor cells in soft agar hold promise as valuable modalities for a variety of diagnostic studies, which include morphological distinction between tumor types by electron microscopy (EM). We present here two cases in which cells in culture expressed distinct morphological features not apparent in the original biopsy specimen. Evaluation of the original biopsies by light and electron microscopy indicated both neoplasms to be undifferentiated sarcomas. Colonies of cells propagated in soft agar displayed features of rhabdomyoblasts in one case, and cultured cells of the second biopsy expressed features of Ewing's sarcoma.

Texture measurements in Al2O3 using BEKP

1994 ◽  
Vol 52 ◽  
pp. 608-609
Author(s):  
M. D. Vaudin ◽  
J. P. Cline
Keyword(s):  
Neutron Diffraction ◽  
Rapid Method ◽  
Crystallographic Orientation ◽  
Specimen Surface ◽  
X Ray Diffraction ◽  
Anisotropic Crystal ◽  
X Ray ◽  
Verification Method ◽  
Crystal Properties ◽  
Backscattered Electron

The study of preferred crystallographic orientation (texture) in ceramics is assuming greater importance as their anisotropic crystal properties are being used to advantage in an increasing number of applications. The quantification of texture by a reliable and rapid method is required. Analysis of backscattered electron Kikuchi patterns (BEKPs) can be used to provide the crystallographic orientation of as many grains as time and resources allow. The technique is relatively slow, particularly for noncubic materials, but the data are more accurate than any comparable technique when a sufficient number of grains are analyzed. Thus, BEKP is well-suited as a verification method for data obtained in faster ways, such as x-ray or neutron diffraction. We have compared texture data obtained using BEKP, x-ray diffraction and neutron diffraction. Alumina specimens displaying differing levels of axisymmetric (0001) texture normal to the specimen surface were investigated.BEKP patterns were obtained from about a hundred grains selected at random in each specimen.

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