Intracellular matrix metalloproteinase-9 mediates epigenetic modifications and autophagy to regulate differentiation in human cardiac stem cells

Stem Cells ◽  
2021 ◽  
Vol 39 (4) ◽  
pp. 497-506
Author(s):  
Santosh K. Yadav ◽  
Paras K. Mishra
Keyword(s):  
Stem Cells ◽  
Matrix Metalloproteinase ◽  
Epigenetic Modifications ◽  
Matrix Metalloproteinase 9 ◽  
Cardiac Stem Cells ◽  
Metalloproteinase 9

scholarly journals Resistin promotes cardiac homing of mesenchymal stem cells and functional recovery after myocardial ischemia-reperfusion via the ERK1/2-MMP-9 pathway

2019 ◽  
Vol 316 (1) ◽  
pp. H233-H244 ◽  
Author(s):  
Yuan He ◽  
Yanjie Guo ◽  
Yunlong Xia ◽  
Yongzhen Guo ◽  
Rutao Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Myocardial Ischemia ◽  
Matrix Metalloproteinase ◽  
Ischemia Reperfusion ◽  
Matrix Metalloproteinase 9 ◽  
Myocardial Ischemia Reperfusion ◽  
And Migration ◽  
Metalloproteinase 9 ◽  
Proliferation And Migration

Stem cell therapy is a potentially effective and promising treatment for ischemic heart disease. Resistin, a type of adipokine, has been found to bind to adipose-derived mesenchymal stem cells (ADSCs). However, the effects of resistin on cardiac homing by ADSCs and on ADSC-mediated cardioprotective effects have not been investigated. ADSCs were obtained from enhanced green fluorescent protein transgenic mice. C57BL/6J mice were subjected to myocardial ischemia-reperfusion (I/R) or sham operations. Six hours after the I/R operation, mice were intravenously injected with resistin-treated ADSCs (ADSC-resistin) or vehicle-treated ADSCs (ADSC-vehicle). Cardiac homing by ADSCs and cardiomyocyte apoptosis were investigated 3 days after I/R. Cardiac function, fibrosis, and angiogenesis were evaluated 4 wk after I/R. Cellular and molecular mechanisms were investigated in vitro using cultured ADSCs. Both immunostaining and flow cytometric experiments showed that resistin treatment promoted ADSC myocardial homing 3 days after intravenous injection. Echocardiographic experiments showed that ADSC-resistin, but not ADSC-vehicle, significantly improved left ventricular ejection fraction. ADSC-resistin transplantation significantly mitigated I/R-induced fibrosis and reduced atrial natriuretic peptide/brain natriuretic peptide mRNA expression. In addition, cardiomyocyte apoptosis was reduced, whereas angiogenesis was increased by ADSC-resistin treatment. At the cellular level, resistin promoted ADSC proliferation and migration but did not affect H2O2-induced apoptosis. Molecular experiments identified the ERK1/2-matrix metalloproteinase-9 pathway as a key component mediating the effects of resistin on ADSC proliferation and migration. These results demonstrate that resistin can promote homing of injected ADSCs into damaged heart tissue and stimulate functional recovery, an effect mediated through the ERK1/2 signaling pathway and matrix metalloproteinase-9. NEW & NOTEWORTHY First, intravenous injection of adipose-derived mesenchymal stem cells (ADSCs) treated with resistin significantly increased angiogenesis and reduced myocardial apoptosis and fibrosis in a murine model of ischemia-reperfusion, resulting in improved cardiac performance. Second, resistin treatment significantly increased myocardial homing of intravenously delivered ADSCs. Finally, the ERK1/2-matrix metalloproteinase 9 pathway contributed to the higher proliferative and migratory capacities of ADSCs treated with resistin.

Matrix metalloproteinase-9 and cell kinetics during the collection of peripheral blood stem cells by leukapheresis

2005 ◽  
Vol 33 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Dragoslav Domanović ◽  
Gordana Wozniak ◽  
Peter Černelč ◽  
Marina Samardžija ◽  
Sanja Balen-Marunić ◽  
...  
Keyword(s):  
Stem Cells ◽  
Matrix Metalloproteinase ◽  
Peripheral Blood ◽  
Cell Kinetics ◽  
Matrix Metalloproteinase 9 ◽  
Peripheral Blood Stem Cells ◽  
Blood Stem Cells ◽  
Metalloproteinase 9

473 Adenosine stimulates matrix metalloproteinase-9 secretion by THP-1-derived macrophages

2007 ◽  
Vol 6 (1) ◽  
pp. 102-102
Author(s):  
E VELOT ◽  
I ERNENS ◽  
B HAAS ◽  
C JEANTY ◽  
D ROUY ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 9 ◽  
Metalloproteinase 9
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