scholarly journals Identification of the Coiled-Coil Domain as an Essential Methyl-CpG-Binding Domain Protein 3 Element for Preserving Lineage Commitment Potential of Embryonic Stem Cells

Stem Cells ◽  
2018 ◽  
Vol 36 (9) ◽  
pp. 1355-1367 ◽  
Author(s):  
Masataka Hirasaki ◽  
Atsushi Ueda ◽  
Masamitsu N. Asaka ◽  
Kousuke Uranishi ◽  
Ayumu Suzuki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Coiled Coil ◽  
Binding Domain ◽  
Lineage Commitment ◽  
Coiled Coil Domain ◽  
Domain Protein

scholarly journals Self-Renewal Versus Lineage Commitment of Embryonic Stem Cells: Protein Kinase C Signaling Shifts the Balance

Stem Cells ◽  
2011 ◽  
Vol 29 (4) ◽  
pp. 618-628 ◽  
Author(s):  
Debasree Dutta ◽  
Soma Ray ◽  
Pratik Home ◽  
Melissa Larson ◽  
Michael W. Wolfe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Kinase C ◽  
Embryonic Stem Cells ◽  
Protein Kinase ◽  
Embryonic Stem ◽  
Lineage Commitment ◽  
Self Renewal ◽  
Kinase C

scholarly journals RUNX1a enhances hematopoietic lineage commitment from human embryonic stem cells and inducible pluripotent stem cells

Blood ◽  
2013 ◽  
Vol 121 (15) ◽  
pp. 2882-2890 ◽  
Author(s):  
Dan Ran ◽  
Wei-Jong Shia ◽  
Miao-Chia Lo ◽  
Jun-Bao Fan ◽  
David A. Knorr ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Pluripotent Stem Cells ◽  
Ex Vivo ◽  
Ectopic Expression ◽  
Embryonic Stem ◽  
Embryoid Bodies ◽  
Lineage Commitment ◽  
Hematopoietic Differentiation

Abstract Advancements in human pluripotent stem cell (hPSC) research have potential to revolutionize therapeutic transplantation. It has been demonstrated that transcription factors may play key roles in regulating maintenance, expansion, and differentiation of hPSCs. In addition to its regulatory functions in hematopoiesis and blood-related disorders, the transcription factor RUNX1 is also required for the formation of definitive blood stem cells. In this study, we demonstrated that expression of endogenous RUNX1a, an isoform of RUNX1, parallels with lineage commitment and hematopoietic emergence from hPSCs, including both human embryonic stem cells and inducible pluripotent stem cells. In a defined hematopoietic differentiation system, ectopic expression of RUNX1a facilitates emergence of hematopoietic progenitor cells (HPCs) and positively regulates expression of mesoderm and hematopoietic differentiation-related factors, including Brachyury, KDR, SCL, GATA2, and PU.1. HPCs derived from RUNX1a hPSCs show enhanced expansion ability, and the ex vivo–expanded cells are capable of differentiating into multiple lineages. Expression of RUNX1a in embryoid bodies (EBs) promotes definitive hematopoiesis that generates erythrocytes with β-globin production. Moreover, HPCs generated from RUNX1a EBs possess ≥9-week repopulation ability and show multilineage hematopoietic reconstitution in vivo. Together, our results suggest that RUNX1a facilitates the process of producing therapeutic HPCs from hPSCs.

scholarly journals Fam40b is required for lineage commitment of murine embryonic stem cells

2014 ◽  
Vol 5 (7) ◽  
pp. e1320-e1320 ◽  
Author(s):  
V Wagh ◽  
M X Doss ◽  
D Sabour ◽  
R Niemann ◽  
K Meganathan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Lineage Commitment ◽  
Murine Embryonic Stem Cells

scholarly journals Transcriptional analysis of early lineage commitment in human embryonic stem cells

2007 ◽  
Vol 7 (1) ◽  
pp. 12 ◽  
Author(s):  
Andrew L Laslett ◽  
Sean Grimmond ◽  
Brooke Gardiner ◽  
Lincon Stamp ◽  
Adelia Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Transcriptional Analysis ◽  
Lineage Commitment

scholarly journals A Continuum of Cell States Spans Pluripotency and Lineage Commitment in Human Embryonic Stem Cells

PLoS ONE ◽  
2009 ◽  
Vol 4 (11) ◽  
pp. e7708 ◽  
Author(s):  
Shelley R. Hough ◽  
Andrew L. Laslett ◽  
Sean B. Grimmond ◽  
Gabriel Kolle ◽  
Martin F. Pera
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Lineage Commitment
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