scholarly journals Transplantation of Neural Progenitor Cells Expressing Glial Cell Line-Derived Neurotrophic Factor into the Motor Cortex as a Strategy to Treat Amyotrophic Lateral Sclerosis

Stem Cells ◽  
2018 ◽  
Vol 36 (7) ◽  
pp. 1122-1131 ◽  
Author(s):  
Gretchen M. Thomsen ◽  
Pablo Avalos ◽  
Annie A. Ma ◽  
Mor Alkaslasi ◽  
Noell Cho ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Motor Cortex ◽  
Cell Line ◽  
Progenitor Cells ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Lateral Sclerosis

Glial cell line-derived neurotrophic factor enhances in vitro differentiation of mid-/hindbrain neural progenitor cells to dopaminergic-like neurons

2010 ◽  
Vol 88 (15) ◽  
pp. 3222-3232 ◽  
Author(s):  
Amber Young ◽  
Kristin S. Assey ◽  
Carla D. Sturkie ◽  
Franklin D. West ◽  
David W. Machacek ◽  
...  
Keyword(s):  
Cell Line ◽  
Progenitor Cells ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
In Vitro Differentiation

scholarly journals Efficient Transduction of Feline Neural Progenitor Cells for Delivery of Glial Cell Line-Derived Neurotrophic Factor Using a Feline Immunodeficiency Virus-Based Lentiviral Construct

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
X. Joann You ◽  
Ping Gu ◽  
Jinmei Wang ◽  
Tianran Song ◽  
Jing Yang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Degenerative Disease ◽  
Before And After ◽  
A Cell

Work has shown that stem cell transplantation can rescue or replace neurons in models of retinal degenerative disease. Neural progenitor cells (NPCs) modified to overexpress neurotrophic factors are one means of providing sustained delivery of therapeutic gene products in vivo. To develop a nonrodent animal model of this therapeutic strategy, we previously derived NPCs from the fetal cat brain (cNPCs). Here we use bicistronic feline lentiviral vectors to transduce cNPCs with glial cell-derived neurotrophic factor (GDNF) together with a GFP reporter gene. Transduction efficacy is assessed, together with transgene expression level and stability during induction of cellular differentiation, together with the influence of GDNF transduction on growth and gene expression profile. We show that GDNF overexpressing cNPCs expand in vitro, coexpress GFP, and secrete high levels of GDNF protein—before and after differentiation—all qualities advantageous for use as a cell-based approach in feline models of neural degenerative disease.

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