scholarly journals Concise Review: Criteria for Chamber-Specific Categorization of Human Cardiac Myocytes Derived from Pluripotent Stem Cells

Stem Cells ◽  
2017 ◽  
Vol 35 (8) ◽  
pp. 1881-1897 ◽  
Author(s):  
Christopher Kane ◽  
Cesare M. N. Terracciano
Keyword(s):  
Stem Cells ◽  
Cardiac Myocytes ◽  
Pluripotent Stem Cells ◽  
Concise Review ◽  
Review Criteria

scholarly journals Concise Review: Molecular Cytogenetics and Quality Control: Clinical Guardians for Pluripotent Stem Cells

2018 ◽  
Vol 7 (12) ◽  
pp. 867-875 ◽  
Author(s):  
Leili Rohani ◽  
Adiv A. Johnson ◽  
Pooyan Naghsh ◽  
Derrick E. Rancourt ◽  
Henning Ulrich ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quality Control ◽  
Pluripotent Stem Cells ◽  
Molecular Cytogenetics ◽  
Concise Review

scholarly journals Paracrine Effects of the Pluripotent Stem Cell-Derived Cardiac Myocytes Salvage the Injured Myocardium

2017 ◽  
Vol 121 (6) ◽  
Author(s):  
Atsushi Tachibana ◽  
Michelle R. Santoso ◽  
Morteza Mahmoudi ◽  
Praveen Shukla ◽  
Lei Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Left Ventricular Ejection Fraction ◽  
Cardiac Myocytes ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Left Ventricular ◽  
Left Ventricular Ejection ◽  
Ventricular Ejection Fraction ◽  
Paracrine Effects

Rationale: Cardiac myocytes derived from pluripotent stem cells have demonstrated the potential to mitigate damage of the infarcted myocardium and improve left ventricular ejection fraction. However, the mechanism underlying the functional benefit is unclear. Objective: To evaluate whether the transplantation of cardiac-lineage differentiated derivatives enhance myocardial viability and restore left ventricular ejection fraction more effectively than undifferentiated pluripotent stem cells after a myocardial injury. Herein, we utilize novel multimodality evaluation of human embryonic stem cells (hESCs), hESC-derived cardiac myocytes (hCMs), human induced pluripotent stem cells (iPSCs), and iPSC-derived cardiac myocytes (iCMs) in a murine myocardial injury model. Methods and Results: Permanent ligation of the left anterior descending coronary artery was induced in immunosuppressed mice. Intramyocardial injection was performed with (1) hESCs (n=9), (2) iPSCs (n=8), (3) hCMs (n=9), (4) iCMs (n=14), and (5) PBS control (n=10). Left ventricular ejection fraction and myocardial viability, measured by cardiac magnetic resonance imaging and manganese-enhanced magnetic resonance imaging, respectively, was significantly improved in hCM- and iCM-treated mice compared with pluripotent stem cell- or control-treated mice. Bioluminescence imaging revealed limited cell engraftment in all treated groups, suggesting that the cell secretions may underlie the repair mechanism. To determine the paracrine effects of the transplanted cells, cytokines from supernatants from all groups were assessed in vitro. Gene expression and immunohistochemistry analyses of the murine myocardium demonstrated significant upregulation of the promigratory, proangiogenic, and antiapoptotic targets in groups treated with cardiac lineage cells compared with pluripotent stem cell and control groups. Conclusions: This study demonstrates that the cardiac phenotype of hCMs and iCMs salvages the injured myocardium effectively than undifferentiated stem cells through their differential paracrine effects.

scholarly journals Concise Review: Cardiac Disease Modeling Using Induced Pluripotent Stem Cells

Stem Cells ◽  
2015 ◽  
Vol 33 (9) ◽  
pp. 2643-2651 ◽  
Author(s):  
Chunbo Yang ◽  
Jumana Al-Aama ◽  
Miodrag Stojkovic ◽  
Bernard Keavney ◽  
Andrew Trafford ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cardiac Disease ◽  
Disease Modeling ◽  
Concise Review ◽  
Induced Pluripotent

scholarly journals Concise Review: Kidney Generation with Human Pluripotent Stem Cells

Stem Cells ◽  
2017 ◽  
Vol 35 (11) ◽  
pp. 2209-2217 ◽  
Author(s):  
Ryuji Morizane ◽  
Tomoya Miyoshi ◽  
Joseph V. Bonventre
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Concise Review

scholarly journals Concise Review: Epidermal Grafting: The Case for Pluripotent Stem Cells

Stem Cells ◽  
2011 ◽  
Vol 29 (6) ◽  
pp. 895-899 ◽  
Author(s):  
Gilles Lemaître ◽  
Xavier Nissan ◽  
Christine Baldeschi ◽  
Marc Peschanski
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Concise Review

scholarly journals Concise Review: Human Pluripotent Stem Cells to Produce Cell-Based Cancer Immunotherapy

Stem Cells ◽  
2018 ◽  
Vol 36 (2) ◽  
pp. 134-145 ◽  
Author(s):  
Huang Zhu ◽  
Yi-Shin Lai ◽  
Ye Li ◽  
Robert H. Blum ◽  
Dan S. Kaufman
Keyword(s):  
Stem Cells ◽  
Cancer Immunotherapy ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Produce Cell ◽  
Concise Review

scholarly journals Induced Pluripotent Stem Cells Technology and Cardiomyocyte Generation: Progress, Uncertainties and Challenges in the Biological Features and Clinical Applications

2018 ◽  
Author(s):  
Angela Di Baldassarre ◽  
Elisa Cimetta ◽  
Sveva Bollini ◽  
Giulia Gaggi ◽  
Barbara Ghinassi
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Cardiac Myocytes ◽  
Pluripotent Stem Cells ◽  
Cardiac Tissue ◽  
Embryonic Stem ◽  
Cardiac Regeneration ◽  
Paracrine Effects ◽  
Phenotypic Differences ◽  
Induced Pluripotent

Human induced pluripotent stem cells (hiPSCs) are reprogrammed cells that have hallmarks similar to embryonic stem cells including the capacity of self-renewal and differentiation into cardiac myocytes. The improvements in reprogramming and differentiating methods achieved in the past 10 years widened the use of hiPSCs, especially in cardiac research. hiPSC-derived cardiac myocytes (CMs) recapitulate phenotypic differences caused by genetic variations, making them human attractive disease models and useful tools for drug discovery and toxicology testing. In addition, hiPSCs can be used as source cells for cardiac regeneration in animal models. Here, we review the advances in the genetic and epigenetic control of cardiomyogenesis that underlies the significant improvement of the induced reprogramming of somatic cells to CMs. We also cover the phenotypic characteristics of the hiPSCs derived CMs, their ability to rescue injured CMs through paracrine effects, the novel approaches in tissue engineering for hiPSC-derived cardiac tissue generation, and finally, their potential use in biomedical applications.

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