scholarly journals Multifactorial Experimental Design to Optimize the Anti-Inflammatory and Proangiogenic Potential of Mesenchymal Stem Cell Spheroids

Stem Cells ◽  
2017 ◽  
Vol 35 (6) ◽  
pp. 1493-1504 ◽  
Author(s):  
Kaitlin C. Murphy ◽  
Jacklyn Whitehead ◽  
Patrick C. Falahee ◽  
Dejie Zhou ◽  
Scott I. Simon ◽  
...  
Keyword(s):  
Stem Cell ◽  
Experimental Design ◽  
Mesenchymal Stem Cell ◽  
Cell Spheroids ◽  
Anti Inflammatory

scholarly journals Intravenous administration of anti-inflammatory mesenchymal stem cell spheroids reduces chronic alcohol intake and abolishes binge-drinking

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Fernando Ezquer ◽  
Paola Morales ◽  
María Elena Quintanilla ◽  
Daniela Santapau ◽  
Carolyne Lespay-Rebolledo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Binge Drinking ◽  
Intravenous Administration ◽  
Alcohol Intake ◽  
Chronic Alcohol ◽  
Cell Spheroids ◽  
Anti Inflammatory

Mesenchymal Stem Cell Conditioned Medium as Good as Methyl Prednisolone in Decreasing Levels of Interleukin 10 and The Degree of Pulmonary Vasculitis in Lupus Mice

2021 ◽  
Vol 20 (2) ◽  
pp. 426-430
Author(s):  
Nurhasan Agung Prabowo ◽  
Zainal Arifin Adnan ◽  
Arief Nurudhin ◽  
Yulyani Werdiningsih ◽  
Kukuh Prasetyo
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Conditioned Medium ◽  
Interleukin 10 ◽  
Control Group ◽  
Standard Drug ◽  
Methyl Prednisolone ◽  
Pulmonary Vasculitis ◽  
Anti Inflammatory ◽  
Cell Conditioned Medium

Background: Systemic lupus erythematosus is a chronic autoimmune disease that affects target organs. mesenchymal stem cell conditioned medium has immunosuppressive, anti-inflammatory, and immunoregulatory properties in lupus. Methyl prednisolone is a standard drug for lupus with immunosuppressive and anti-inflammatory properties. This study aims to compare the therapeutic effect of mesenchymal stem cell conditioned medium administration compared to methyl prednisolone on interleukin 10 levels and the degree of pulmonary vasculitis of lupus mice. Methods: The subjects were 24 female mice of Mus musculus Balb/C strain, which were categorized into 4 groups of 8 mice, i.e. the control group receiving 0.5 cc of 0.9% NaCl injection and placebo, the lupus group receiving 0.5 cc of pristane injection and placebo, and the treatment mesenchymal stem cell conditioned medium group receiving 0.5 cc pristane injection and mesenchymal stem cell conditioned medium 0,5 cc, and methylprednisolone group receiving 0,5 cc pristiane injection and methylprednisolone p.o 1,5 mg/kgbodyweight. After 24 days the mice were terminated and kidney and blood samples were taken. Statistical analysis was performed using ANOVA test followed by independent T-test. The p value was considered significant when the p < 0.05. Results: The study showed that there was no difference on the levels of interleukin level10 among mesenchymal stem cell conditioned medium goup and methyl prednisolone group (CM = 5,94 ± 2,49 pg/mL, mp = 5,86+1,73 pg/mL; p = 1) and the degree of pulmonary vasculitis (CM= 1,94 ± 0,25, MP=1,89+ 0,11 pg/ml; p = 0.667). Mesenchymal stem cell conditioned medium as good as methyl prednisolone in decreasing levels of interleukin 10 and the degree of pulmonary vasculitis in lupus mice. Conclusion: Mesenchymal stem cell conditioned medium as good as methyl prednisolone in decreasing levels of interleukin 10 and the degree of pulmonary vasculitis in lupus mice Bangladesh Journal of Medical Science Vol.20(2) 2021 p.426-430

Scalable and cost-effective generation of osteogenic micro-tissues through the incorporation of inorganic microparticles within mesenchymal stem cell spheroids

2019 ◽  
Vol 12 (1) ◽  
pp. 015021 ◽  
Author(s):  
Ibrahim Zarkesh ◽  
Majid Halvaei ◽  
Mohammad Hossein Ghanian ◽  
Fatemeh Bagheri ◽  
Forough Azam Sayahpour ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cost Effective ◽  
Cell Spheroids ◽  
Effective Generation

Mesenchymal Stem Cell Spheroids Embedded in an Injectable Thermosensitive Hydrogel: An In Situ Drug Formation Platform for Accelerated Wound Healing

2020 ◽  
Vol 6 (9) ◽  
pp. 5096-5109
Author(s):  
Mohammad Ali Nilforoushzadeh ◽  
Mohsen Khodadadi Yazdi ◽  
Shaghayegh Baradaran Ghavami ◽  
Samila Farokhimanesh ◽  
Leila Mohammadi Amirabad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Thermosensitive Hydrogel ◽  
Cell Spheroids

Engineering mesenchymal stem cell spheroids by incorporation of mechanoregulator microparticles

2018 ◽  
Vol 84 ◽  
pp. 74-87 ◽  
Author(s):  
Fatemeh Abbasi ◽  
Mohammad Hossein Ghanian ◽  
Hossein Baharvand ◽  
Bahman Vahidi ◽  
Mohamadreza Baghaban Eslaminejad
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Spheroids

Human neonatal mesenchymal stem cell spheroids-conditioned medium accelerates skin regeneration

2015 ◽  
Vol 238 (2) ◽  
pp. S376-S377 ◽  
Author(s):  
S.P. Camões ◽  
J.M. Santos ◽  
M.M. Gaspar ◽  
S.I. Simões ◽  
R.N. Bárcia ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Conditioned Medium ◽  
Skin Regeneration ◽  
Cell Spheroids

scholarly journals Mesenchymal stem cell therapy efficacy in COVID-19 patients: A systematic review and meta-analysis

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 956
Author(s):  
Andrianto Andrianto ◽  
Desak Ketut Sekar Cempaka Putri ◽  
Makhyan Jibril Al Farabi ◽  
Teuku Yusrizal ◽  
Hanestya Oky Hermawan
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Adverse Events ◽  
Inflammatory Cytokines ◽  
Meta Analysis ◽  
Group Versus ◽  
Mechanical Ventilators ◽  
Mesenchymal Stem Cell Therapy ◽  
Anti Inflammatory ◽  
Pro Inflammatory Cytokines

Objective: To evaluate mesenchymal stem cell (MSC) administration safety and efficacy in COVID-19 patients. Methods: We conducted a literature search on PubMed/MEDLINE, medRxiv, EBSCOhost/CINAHL, ProQuest, and Scopus with keywords adjusted to each search engine’s specifications on February 12, 2021. Interventional studies that reviewed MSC efficacy (mortality, hospitalization duration, need for mechanical ventilators, and inflammation markers) and/or safety (adverse events) in COVID-19 patients who were 18 years old or more were included in this study. Study eligibility, data extraction, and study quality assessment were conducted independently by each author. Results: A total of five studies of moderate to high quality with a total of 193 patients were included. One of the three randomized studies included did not apply blinding to either participants or medical professionals. Pooled OR (Odd Ratio) for mortality risk, adverse events incidence, and use of mechanical ventilators for patients on MSC therapy were 0.13 [95% CI: 0.02, 0.68], 0.91 [95% CI: 0.45, 1.86], and 0.42 [95% CI: 0.12, 1.47], respectively. Pooled mean difference for hospitalization duration in the MSC group versus the control was -3.54 [CI 95%: -4.68, -2.40] with 7% heterogeneity. All studies agreed that there was an increase of pro-inflammatory cytokines and a decrease of anti-inflammatory markers that were statistically different in the MSC group. Conclusion: Mesenchymal stem cell administration to COVID-19 patients is safe and effective in reducing mortality and hospitalization duration. Furthermore, a decrease of pro-inflammatory cytokines and an increase in anti-inflammatory cytokines were observed.

Sign in / Sign up

Export Citation Format

Share Document