Concise Review: Signaling Control of Early Fate Decisions Around the Human Pluripotent Stem Cell State

Stem Cells ◽  
2016 ◽  
Vol 35 (2) ◽  
pp. 277-283 ◽  
Author(s):  
Jyoti Rao ◽  
Boris Greber
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Cell State ◽  
Concise Review ◽  
Stem Cell State

scholarly journals Glycolytic Metabolism Plays a Functional Role in Regulating Human Pluripotent Stem Cell State

2016 ◽  
Vol 19 (4) ◽  
pp. 476-490 ◽  
Author(s):  
Wen Gu ◽  
Xavier Gaeta ◽  
Anna Sahakyan ◽  
Alanna B. Chan ◽  
Candice S. Hong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Functional Role ◽  
Pluripotent Stem Cell ◽  
Glycolytic Metabolism ◽  
Human Pluripotent Stem Cell ◽  
Cell State ◽  
Stem Cell State

Clonal Isolation of an Intermediate Pluripotent Stem Cell State

Stem Cells ◽  
2013 ◽  
Vol 31 (5) ◽  
pp. 918-927 ◽  
Author(s):  
Kuo-Hsuan Chang ◽  
Meng Li
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cell State ◽  
Stem Cell State

scholarly journals Generation and trapping of a mesoderm biased state of human pluripotency

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Dylan Stavish ◽  
Charlotta Böiers ◽  
Christopher Price ◽  
Thomas J. R. Frith ◽  
Jason Halliwell ◽  
...  
Keyword(s):  
Stem Cell ◽  
Lysophosphatidic Acid ◽  
Pluripotent Stem Cell ◽  
Efficient Production ◽  
Human Pluripotent Stem Cell ◽  
Cell Level ◽  
Cell Compartment ◽  
General Applicability ◽  
Stem Cell Compartment ◽  
Stem Cell State

Abstract We postulate that exit from pluripotency involves intermediates that retain pluripotency while simultaneously exhibiting lineage-bias. Using a MIXL1 reporter, we explore mesoderm lineage-bias within the human pluripotent stem cell compartment. We identify a substate, which at the single cell level coexpresses pluripotent and mesodermal gene expression programmes. Functionally these cells initiate stem cell cultures and exhibit mesodermal bias in differentiation assays. By promoting mesodermal identity through manipulation of WNT signalling while preventing exit from pluripotency using lysophosphatidic acid, we ‘trap’ and maintain cells in a lineage-biased stem cell state through multiple passages. These cells correspond to a normal state on the differentiation trajectory, the plasticity of which is evidenced by their reacquisition of an unbiased state upon removal of differentiation cues. The use of ‘cross-antagonistic’ signalling to trap pluripotent stem cell intermediates with different lineage-bias may have general applicability in the efficient production of cells for regenerative medicine.

scholarly journals Concise Review: Maturation Phases of Human Pluripotent Stem Cell-Derived Cardiomyocytes

Stem Cells ◽  
2013 ◽  
Vol 31 (5) ◽  
pp. 829-837 ◽  
Author(s):  
Claire Robertson ◽  
David D. Tran ◽  
Steven C. George
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Concise Review

scholarly journals Smad2 Is Essential for Maintenance of the Human and Mouse Primed Pluripotent Stem Cell State

2013 ◽  
Vol 288 (25) ◽  
pp. 18546-18560 ◽  
Author(s):  
Masayo Sakaki-Yumoto ◽  
Jianming Liu ◽  
Miguel Ramalho-Santos ◽  
Nobuaki Yoshida ◽  
Rik Derynck
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cell State ◽  
Stem Cell State ◽  
Human And Mouse

scholarly journals Generation and Trapping of a Mesoderm Biased State of Human Pluripotency

2019 ◽  
Author(s):  
Dylan Stavish ◽  
Charlotta Böiers ◽  
Christopher Price ◽  
Thomas J R Frith ◽  
Jason Halliwell ◽  
...  
Keyword(s):  
Stem Cell ◽  
Lysophosphatidic Acid ◽  
Pluripotent Stem Cell ◽  
Efficient Production ◽  
Human Pluripotent Stem Cell ◽  
Cell Level ◽  
Cell Compartment ◽  
General Applicability ◽  
Stem Cell Compartment ◽  
Stem Cell State

ABSTRACTWe postulate that exit from pluripotency involves intermediates that retain pluripotency while simultaneously exhibiting lineage-bias. Using a MIXL1 reporter, we explored mesoderm lineage-bias within the human pluripotent stem cell compartment. We identified a substate, which at the single cell level coexpresses pluripotent and mesodermal gene expression programs. Functionally these cells could initiate stem cell cultures and exhibited mesodermal bias in differentiation assays. By promoting mesodermal identity through manipulation of WNT signalling while preventing exit from pluripotency using lysophosphatidic acid, we could ‘trap’ and maintain cells in a lineage-biased stem cell state through multiple passages. These cells correspond to a normal state on the differentiation trajectory, the plasticity of which is evidenced by their reacquisition of an unbiased state upon removal of differentiation cues. The use of ‘cross-antagonistic’ signalling to trap pluripotent stem cell intermediates with different lineage-bias may have general applicability in the efficient production of cells for regenerative medicine.

scholarly journals Coupling of X-Chromosome reactivation with the pluripotent stem cell state

RNA Biology ◽  
2014 ◽  
Vol 11 (7) ◽  
pp. 798-807 ◽  
Author(s):  
Bernhard Payer ◽  
Jeannie T Lee
Keyword(s):  
Stem Cell ◽  
X Chromosome ◽  
Pluripotent Stem Cell ◽  
Cell State ◽  
Stem Cell State
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