Activation of Signal Transducers and Activators of Transcription 3 and Focal Adhesion Kinase by Stromal Cell-Derived Factor 1 Is Required for Migration of Human Mesenchymal Stem Cells in Response to Tumor Cell-Conditioned Medium

Stem Cells ◽  
2009 ◽  
Vol 27 (4) ◽  
pp. 857-865 ◽  
Author(s):  
Hui Gao ◽  
Waldemar Priebe ◽  
John Glod ◽  
Debabrata Banerjee
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tumor Cell ◽  
Focal Adhesion ◽  
Stromal Cell ◽  
Human Mesenchymal Stem Cells ◽  
Signal Transducers ◽  
Stromal Cell Derived Factor ◽  
Transcription 3 ◽  
Cell Conditioned Medium

scholarly journals Let-7f miRNA regulates SDF-1α- and hypoxia-promoted migration of mesenchymal stem cells and attenuates mammary tumor growth upon exosomal release

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Virginia Egea ◽  
Kai Kessenbrock ◽  
Devon Lawson ◽  
Alexander Bartelt ◽  
Christian Weber ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tumor Growth ◽  
Molecular Mechanisms ◽  
Hypoxia Inducible Factor ◽  
Human Mesenchymal Stem Cells ◽  
Target Cells ◽  
Autophagic Flux ◽  
Stromal Cell Derived Factor

AbstractBone marrow-derived human mesenchymal stem cells (hMSCs) are recruited to damaged or inflamed tissues where they contribute to tissue repair. This multi-step process involves chemokine-directed invasion of hMSCs and on-site release of factors that influence target cells or tumor tissues. However, the underlying molecular mechanisms are largely unclear. Previously, we described that microRNA let-7f controls hMSC differentiation. Here, we investigated the role of let-7f in chemotactic invasion and paracrine anti-tumor effects. Incubation with stromal cell-derived factor-1α (SDF-1α) or inflammatory cytokines upregulated let-7f expression in hMSCs. Transfection of hMSCs with let-7f mimics enhanced CXCR4-dependent invasion by augmentation of pericellular proteolysis and release of matrix metalloproteinase-9. Hypoxia-induced stabilization of the hypoxia-inducible factor 1 alpha in hMSCs promoted cell invasion via let-7f and activation of autophagy. Dependent on its endogenous level, let-7f facilitated hMSC motility and invasion through regulation of the autophagic flux in these cells. In addition, secreted let-7f encapsulated in exosomes was increased upon upregulation of endogenous let-7f by treatment of the cells with SDF-1α, hypoxia, or induction of autophagy. In recipient 4T1 tumor cells, hMSC-derived exosomal let-7f attenuated proliferation and invasion. Moreover, implantation of 3D spheroids composed of hMSCs and 4T1 cells into a breast cancer mouse model demonstrated that hMSCs overexpressing let-7f inhibited tumor growth in vivo. Our findings provide evidence that let-7f is pivotal in the regulation of hMSC invasion in response to inflammation and hypoxia, suggesting that exosomal let-7f exhibits paracrine anti-tumor effects.

scholarly journals Stromal cell-derived factor 1/CXCR4 signaling is critical for the recruitment of mesenchymal stem cells to the fracture site during skeletal repair in a mouse model

2009 ◽  
Vol 60 (3) ◽  
pp. 813-823 ◽  
Author(s):  
Toshiyuki Kitaori ◽  
Hiromu Ito ◽  
Edward M. Schwarz ◽  
Ryosuke Tsutsumi ◽  
Hiroyuki Yoshitomi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mouse Model ◽  
Stromal Cell ◽  
Fracture Site ◽  
Cxcr4 Signaling ◽  
Stromal Cell Derived Factor

scholarly journals Mesenchymal Stem Cells with Increased Stromal Cell-Derived Factor 1 Expression Enhanced Fracture Healing

2015 ◽  
Vol 21 (3-4) ◽  
pp. 594-602 ◽  
Author(s):  
Chih-Yuan Ho ◽  
Anita Sanghani ◽  
Jia Hua ◽  
Melanie Coathup ◽  
Priya Kalia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fracture Healing ◽  
Stromal Cell ◽  
Stromal Cell Derived Factor

scholarly journals NF-κB downregulation may be involved the depression of tumor cell proliferation mediated by human mesenchymal stem cells

2008 ◽  
Vol 29 (3) ◽  
pp. 333-340 ◽  
Author(s):  
Ling QIAO ◽  
Tie-jun ZHAO ◽  
Feng-ze WANG ◽  
Chang-liang SHAN ◽  
Li-hong YE ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Tumor Cell ◽  
Human Mesenchymal Stem Cells ◽  
Tumor Cell Proliferation
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