scholarly journals Human Salivary Gland Stem Cells Functionally Restore Radiation Damaged Salivary Glands

Stem Cells ◽  
2016 ◽  
Vol 34 (3) ◽  
pp. 640-652 ◽  
Author(s):  
Sarah Pringle ◽  
Martti Maimets ◽  
Marianne van der Zwaag ◽  
Monique A. Stokman ◽  
Djoke van Gosliga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Human Salivary Gland

scholarly journals Human salivary gland stem cells ameliorate hyposalivation of radiation-damaged rat salivary glands

2013 ◽  
Vol 45 (11) ◽  
pp. e58-e58 ◽  
Author(s):  
Jaemin Jeong ◽  
Hyunjung Baek ◽  
Yoon-Ju Kim ◽  
Youngwook Choi ◽  
Heekyung Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Human Salivary Gland ◽  
Rat Salivary Glands

Isolation of Human Salivary Gland Stem Cells from Submandibular Glands

2010 ◽  
Vol 78 (3) ◽  
pp. S81
Author(s):  
A. Banh ◽  
H. Cao ◽  
S. Wu ◽  
T.E. Krakow ◽  
M. Yao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Salivary Gland ◽  
Submandibular Glands ◽  
Human Salivary Gland

Exosomes Derived from Bone Marrow-Derived Mesenchymal Stem Cells (BM-MSC) Protect Submandibular Glands in Diabetic Rats

2021 ◽  
Vol 11 (11) ◽  
pp. 2168-2173
Author(s):  
Cong Zhang ◽  
Xiaohong Zhang ◽  
Min Zhang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Serum Amylase ◽  
Diabetic Rats ◽  
Submandibular Salivary Gland ◽  
Amylase Level ◽  
Group I

Our study assess whether exosomes derived from bone marrow mesenchymal stem cells (BM-MSC) ameliorates diabetic salivary gland complications. 10 SD rats were assigned into diabetes group I and exosome treatment group II. Diabetic rats were induced by streptozotocin (STZ) and injected with DMSO or exosomes through tail vein followed by collection of submandibular salivary gland samples for histological analysis and TGFβ, Smad2 and Smad3 level by PCR, saliva IgA and serum amylase level. Compared with control mice, exosome treatment mice showed less fibrosis of the submandibular salivary glands and duct components with a more complete structure. Exosome treatment inhibited TGFβ, Smad2 and Smad3 level to reduce diabetic salivary gland complications, effectively decreased blood sugar level, improved salivary glands function with significantly reduced serum amylase and salivary IgA levels. In conclusion, BM-MSC-derived exosomes may be a new therapeutic strategy for treating diabetic salivary gland complications.

Bone Marrow-Derived Stem Cells Reduce Radiation-Induced Damage to Salivary Glands.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1194-1194
Author(s):  
Pieter K. Wierenga ◽  
Isabelle Lombaert ◽  
Willy Visser ◽  
Harm H. Kampinga ◽  
Gerald de Haan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Laser Fluorescence ◽  
Egfp Expression ◽  
Confocal Laser ◽  
Optimal Time ◽  
Similar Amount ◽  
Radiation Induced

Abstract The salivary glands are often included in the field of irradiation during radiotherapy of head and neck cancer. This can result in severe side-effects that reduces the quality of life of the patient and may even limit the treatment dose. Late damage to the salivary glands is mainly caused by exhaustion of the tissue specific stem cells. Post-irradiation replacement of salivary gland stem cells with donor stem cells may ameliorate radiation-induced complications. Bone marrow-derived stem cells (BMSC) have been shown to be multipotent and thereby able to engraft in many tissues after injury. In this study, we assessed the potential of BMSC to reduce irradiation-induced salivary gland damage. C57BL/6 mice were transplanted with bone marrow from eGFP transgenic animals. After two months the salivary glands of these chimeric mice were locally irradiated with 15 Gy. BMSC were mobilized 10, 30 and/or 60 days after irradiation by s.c. injection of rHu-PEG-G-CSF. Saliva secretion (μl/15 minutes) was measured up to 90 days after irradiation by pilocarpine induction. Hereafter, the glands were extirpated and examined for eGFP-expression. From every individual animal one parotid and one submandibular gland was used to prepare single cell suspensions in order to detect eGFP-positve cells by flow cytometry. The other parotid and submandibular glands were analyzed using confocal laser fluorescence scanning microscopy and light microscopy. G-CSF treatment yielded in an increase of saliva flow for all time points. The optimal time-point for mobilization, however, was 30 days after irradiation as is demonstrated by an improvement of salivary flow from 5 to 30% when compared to radiation alone. FACS analysis showed that up to 10% of the isolated cells were eGFP-positive. Microscopic analysis revealed a similar amount of positive cells and an improved morphology. Immuno-histochemistry using anti-SM-actin antibodies showed the close vicinity of actin and eGFP within the cells, demonstrating the occurence of BMSC derived myoepithelial cells in irradiated salivary glands. Furthermore, using cell-type specific antibodies, the meyoepethilial nature of the eGFP positive was revealed. In conclusion, the results show that BMSC home to severely damaged salivary glands after mobilization. Hence, BMSC mobilization could become a promising modality to ameliorate radiation-induced complications in salivary glands after radiotherapy.

scholarly journals Capability of Tissue Stem Cells to Organize into Salivary Rudiments

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Kenji Okumura ◽  
Masanori Shinohara ◽  
Fumio Endo
Keyword(s):  
Stem Cells ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Progenitor Cells ◽  
Duct Cell ◽  
Branching Morphogenesis ◽  
Embryonic Cells ◽  
Gland Development ◽  
Salivary Epithelia ◽  
Pseudoglandular Stage

Branching morphogenesis (BrM), an essential step for salivary gland development, requires epithelial-mesenchymal interactions. BrM is impaired when the surrounding mesenchyme is detached from the salivary epithelium during the pseudoglandular stage. It is believed that the salivary mesenchyme is indispensable for BrM, however, an extracellular matrix gel with exogenous EGF can be used as a substitute for the mesenchyme during BrM in the developing salivary epithelium. Stem/progenitor cells isolated from salivary glands in humans and rodents can be classified as mesenchymal stem cell-like, bone-marrow-derived, duct cell-like, and embryonic epithelium-like cells. Salivary-gland-derived progenitor (SGP) cells isolated from duct-ligated rats, mice, and swine submandibular glands share similar characteristics, including intracellular laminin andα6β1-integrin expression, similar to the embryonic salivary epithelia during the pseudoglandular stage. Progenitor cells also isolated from human salivary glands (human SGP cells) having the same characteristics differentiate into hepatocyte-like cells when transplanted into the liver. Similar to the dissociated embryonic salivary epithelium, human SGP cells aggregate to self-organize into branching organ-like structures on Matrigel plus exogenous EGF. These results suggest the possibility that tissue stem cells organize rudiment-like structures, and the embryonic cells that organize into whole tissues during development are preserved even in adult tissues.

Embryonic stem cells markers Oct4 and Nanog correlate with perineural invasion in human salivary gland mucoepidermoid carcinoma

2016 ◽  
Vol 46 (2) ◽  
pp. 112-120 ◽  
Author(s):  
Maria Fernanda Setúbal Destro Rodrigues ◽  
Bruno Tavares Sedassari ◽  
Carina Magalhães Esteves ◽  
Nathália Paiva de Andrade ◽  
Albina Altemani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Salivary Gland ◽  
Embryonic Stem Cells ◽  
Mucoepidermoid Carcinoma ◽  
Perineural Invasion ◽  
Embryonic Stem ◽  
Human Salivary Gland

scholarly journals Regenerating Salivary Glands in the Microenvironment of Induced Pluripotent Stem Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Hitomi Ono ◽  
Aya Obana ◽  
Yu Usami ◽  
Manabu Sakai ◽  
Kanji Nohara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Ips Cells ◽  
Initial Attempt ◽  
Cell Niche ◽  
Salivary Gland Regeneration ◽  
Induced Pluripotent

This report describes our initial attempt to regenerate salivary glands using induced pluripotent stem (iPS) cellsin vivoandin vitro. Glandular tissues that were similar to the adult submandibular glands (SMGs) and sublingual glands could be partially produced by the transplantation of iPS cells into mouse salivary glands. However, the tumorigenicity of iPS cells has not been resolved yet. It is well known that stem cells affect their microenvironment, known as a stem cell niche. We focused on the niche and the interaction between iPS cells and salivary gland cells in our study on salivary gland regeneration. Coculture of embryonic SMG cells and iPS cells have better-developed epithelial structures and fewer undifferentiated specific markers than monoculture of embryonic SMG cellsin vitro. These results suggest that iPS cells have a potential ability to accelerate differentiation for salivary gland development and regeneration.

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