A Mesenchymal-Like ZEB1+Niche Harbors Dorsal Radial Glial Fibrillary Acidic Protein-Positive Stem Cells in the Spinal Cord

Stem Cells ◽  
2009 ◽  
Vol 27 (11) ◽  
pp. 2722-2733 ◽  
Author(s):  
Jean-Charles Sabourin ◽  
Karin B. Ackema ◽  
David Ohayon ◽  
Pierre-Olivier Guichet ◽  
Florence E. Perrin ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Glial Fibrillary Acidic Protein ◽  
Acidic Protein ◽  
Radial Glial

scholarly journals The molecular cloning of glial fibrillary acidic protein in Gekko japonicus and its expression changes after spinal cord transection

2010 ◽  
Vol 15 (4) ◽  
Author(s):  
Dehong Gao ◽  
Yongjun Wang ◽  
Yan Liu ◽  
Fei Ding ◽  
Xiaosong Gu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
White Matter ◽  
Glial Fibrillary Acidic Protein ◽  
Acidic Protein ◽  
Time Interval ◽  
Short Time Interval ◽  
Specific Marker ◽  
Class Iii ◽  
Intermediate Filament Proteins ◽  
Gekko Japonicus

AbstractThe glial fibrillary acidic protein (GFAP) is an astrocyte-specific member of the class III intermediate filament proteins. It is generally used as a specific marker of astrocytes in the central nervous system (CNS). We isolated a GFAP cDNA from the brain and spinal cord cDNA library of Gekko japonicus, and prepared polyclonal antibodies against gecko GFAP to provide useful tools for further immunochemistry studies. Both the real-time quantitative PCR and western blot results revealed that the expression of GFAP in the spinal cord after transection increased, reaching its maximum level after 3 days, and then gradually decreased over the rest of the 2 weeks of the experiment. Immunohistochemical analyses demonstrated that the increase in GFAP-positive labeling was restricted to the white matter rather than the gray matter. In particular, a slight increase in the number of GFAP positive star-shaped astrocytes was detected in the ventral and lateral regions of the white matter. Our results indicate that reactive astrogliosis in the gecko spinal cord took place primarily in the white matter during a short time interval, suggesting that the specific astrogliosis evaluated by GFAP expression might be advantageous in spinal cord regeneration.

Lentiviral-mediated silencing of glial fibrillary acidic protein and vimentin promotes anatomical plasticity and functional recovery after spinal cord injury

2014 ◽  
Vol 93 (1) ◽  
pp. 43-55 ◽  
Author(s):  
Mathieu Desclaux ◽  
Florence E. Perrin ◽  
Anh Do-Thi ◽  
Monica Prieto-Cappellini ◽  
Minerva Gimenez y Ribotta ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Glial Fibrillary Acidic Protein ◽  
Functional Recovery ◽  
Acidic Protein ◽  
Anatomical Plasticity ◽  
Cord Injury

scholarly journals Abnormal Reaction to Central Nervous System Injury in Mice Lacking Glial Fibrillary Acidic Protein and Vimentin

1999 ◽  
Vol 145 (3) ◽  
pp. 503-514 ◽  
Author(s):  
Milos Pekny ◽  
Clas B. Johansson ◽  
Camilla Eliasson ◽  
Josefina Stakeberg ◽  
Åsa Wallén ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Central Nervous System ◽  
Nervous System ◽  
Glial Fibrillary Acidic Protein ◽  
Intermediate Filament ◽  
Glial Scar ◽  
Scar Formation ◽  
Brain Lesions ◽  
Acidic Protein ◽  
The Central Nervous System

In response to injury of the central nervous system, astrocytes become reactive and express high levels of the intermediate filament (IF) proteins glial fibrillary acidic protein (GFAP), vimentin, and nestin. We have shown that astrocytes in mice deficient for both GFAP and vimentin (GFAP−/−vim−/−) cannot form IFs even when nestin is expressed and are thus devoid of IFs in their reactive state. Here, we have studied the reaction to injury in the central nervous system in GFAP−/−, vimentin−/−, or GFAP−/−vim−/− mice. Glial scar formation appeared normal after spinal cord or brain lesions in GFAP−/− or vimentin−/− mice, but was impaired in GFAP−/−vim−/− mice that developed less dense scars frequently accompanied by bleeding. These results show that GFAP and vimentin are required for proper glial scar formation in the injured central nervous system and that some degree of functional overlap exists between these IF proteins.

Sign in / Sign up

Export Citation Format

Share Document