scholarly journals Efficient Transcriptional Targeting of Human Hematopoietic Stem Cells and Blood Cell Lineages by Lentiviral Vectors Containing the Regulatory Element of the Wiskott-Aldrich Syndrome Gene

Stem Cells ◽  
2009 ◽  
Vol 27 (11) ◽  
pp. 2815-2823 ◽  
Author(s):  
Valeria Leuci ◽  
Loretta Gammaitoni ◽  
Sonia Capellero ◽  
Dario Sangiolo ◽  
Maria Mesuraca ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Hematopoietic Stem Cells ◽  
Regulatory Element ◽  
Lentiviral Vectors ◽  
Hematopoietic Stem ◽  
Transcriptional Targeting ◽  
Cell Lineages ◽  
Wiskott Aldrich Syndrome

scholarly journals Inflammation and Aging of Hematopoietic Stem Cells in Their Niche

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1849
Author(s):  
Daozheng Yang ◽  
Gerald de Haan
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Blood Cell ◽  
Hematopoietic Stem Cells ◽  
Bone Marrow Niche ◽  
Extrinsic Factors ◽  
Hematopoietic Stem ◽  
Cell Lineages

Hematopoietic stem cells (HSCs) sustain the lifelong production of all blood cell lineages. The functioning of aged HSCs is impaired, including a declined repopulation capacity and myeloid and platelet-restricted differentiation. Both cell-intrinsic and microenvironmental extrinsic factors contribute to HSC aging. Recent studies highlight the emerging role of inflammation in contributing to HSC aging. In this review, we summarize the recent finding of age-associated changes of HSCs and the bone marrow niche in which they lodge, and discuss how inflammation may drive HSC aging.

scholarly journals Identification and Age-dependent Increase of Platelet Biased Human Hematopoietic Stem Cells

2022 ◽  
Author(s):  
Merve Aksoz ◽  
Grigore-Aristide Gafencu ◽  
Bilyana Stoilova Stoilova ◽  
Mario Buono ◽  
Yiran Meng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
Hematological Malignancies ◽  
Hematopoietic Stem ◽  
Cell Lineages ◽  
Cells Of Origin ◽  
Human Individual ◽  
Age Dependent ◽  
Human Hscs

Hematopoietic stem cells (HSC) reconstitute multi-lineage human hematopoiesis after clinical bone marrow transplantation and are the cells-of-origin of hematological malignancies. Though HSC provide multi-lineage engraftment, individual murine HSCs are lineage-biased and contribute unequally to blood cell lineages. Now, by combining xenografting of molecularly barcoded adult human bone marrow (BM) HSCs and high-throughput single cell RNA sequencing we demonstrate that human individual BM HSCs are also functionally and transcriptionally lineage biased. Specifically, we identify platelet-biased and multi-lineage human HSCs. Quantitative comparison of transcriptomes from single HSCs from young, and aged, BM show that both the proportion of platelet-biased HSCs, and their level of transcriptional platelet priming, increases with age. Therefore, platelet-biased HSCs, as well as their increased prevalence and elevated transcriptional platelet priming during ageing, are conserved between human and murine hematopoiesis.

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