scholarly journals Heparanase released from mesenchymal stem cells activates integrin beta1/HIF-2alpha/Flk-1 signaling and promotes endothelial cell migration and angiogenesis

Stem Cells ◽  
2015 ◽  
Vol 33 (6) ◽  
pp. 1850-1862 ◽  
Author(s):  
Xinyang Hu ◽  
Ling Zhang ◽  
Jing Jin ◽  
Wei Zhu ◽  
Yinchuan Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Endothelial Cell Migration ◽  
Integrin Beta1

Abstract 17239: miRNAs Secreted by Exosomes Derived From Adipose Tissue Mesenchymal Stem Cells Overexpressing GATA-4 Increase Endothelial Cell Survival and Promote Angiogenesis

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Jie Xu ◽  
Jiapeng Wang ◽  
Min Liu ◽  
Yigang Wang ◽  
Muhammad Ashraf ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Structure Formation ◽  
Negative Control ◽  
Cell Behavior ◽  
Therapeutic Effects ◽  
Cell Based Therapy

Exosomes (EXO) secreted from stem cells play a critical role in cell based therapy and appear to have greater salutary therapeutic effects than whole cells. Our previous study showed that EXO produced by GATA-4-overexpressing mesenchymal stem cells (MSCs) significantly promoted angiogenesis and protected cardiomyocytes resulting in improved ischemic myocardial function. In the present studies, we compared the miR expression pattern and determined whether Exo GATA-4 delivered miRs particularly promoted angiogenesis. Methods and Results: EXO were collected from conditioned medium of adipose tissue derived MSCs that were transfected with GATA-4 (Exo GATA-4 ) or with vector-empty (Exo null ) using ultracentrifugation ( Fig A ). Functional analysis showed that Exo GATA-4 significantly increased tube formation, migration, and proliferation of human umbilical vein endothelial cells (HUVECs) compared to Exo null . MicroRNA-seq data showed that 7 of the 230 miRs found in EXO, were significantly upregulated in Exo GATA-4 ( Fig B ). To determine the effect of upregulated miRs on Exo GATA-4 mediated angiogenesis, miR-155 and miR-6240 mimic, inhibitor, and negative control were directly transferred into HUVECs. After 48 hours, HUVECs were harvested for measuring endothelial cell behavior. Transfection of miR-6240-mimic significantly increased HUVECs proliferation, tolerance against H 2 O 2 induced injury ( Fig C ), tube-like structure formation ( Fig D ) and cell migration ( Fig E ) compared to those transfected with negative control. Concurrently, no significant differences were found in proliferation, survival, and tube-like structure formation among the cells transfected with miR-155-mimic and negative control, although miR-155-mimic significantly increased cell migration. Conclusion: The upregulated miRs in Exo GATA-4 may variably regulate endothelial cell behavior and the role of various miRs needs to be investigated individually.

scholarly journals DIRECT CELL CONTACT BETWEEN BRAIN ENDOTHELIAL CELLS AND GLIOMA STEM CELLS PROMOTES ENDOTHELIAL CELL MIGRATION

2014 ◽  
Vol 16 (suppl 3) ◽  
pp. iii36-iii36
Author(s):  
C. L. Gladson ◽  
M. E. Burgett ◽  
J. D. Lathia ◽  
P. Roth ◽  
P. Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Endothelial Cell Migration ◽  
Cell Contact ◽  
Glioma Stem Cells ◽  
Brain Endothelial Cells ◽  
Direct Cell

scholarly journals Glioma stem cells enhance endothelial cell migration and proliferation via the Hedgehog pathway

2013 ◽  
Vol 6 (5) ◽  
pp. 1524-1530 ◽  
Author(s):  
GUANG-NING YAN ◽  
YANG-FAN LV ◽  
LANG YANG ◽  
XIAO-HONG YAO ◽  
YOU-HONG CUI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Hedgehog Pathway ◽  
Endothelial Cell Migration ◽  
Glioma Stem Cells ◽  
Cell Migration And Proliferation

scholarly journals Synergy of molecularly mobile polyrotaxane surfaces with endothelial cell co-culture for mesenchymal stem cell mineralization

RSC Advances ◽  
2021 ◽  
Vol 11 (30) ◽  
pp. 18685-18692
Author(s):  
Hiroki Masuda ◽  
Yoshinori Arisaka ◽  
Masahiro Hakariya ◽  
Takanori Iwata ◽  
Tetsuya Yoda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Endothelial Cell ◽  
Mesenchymal Stem Cell ◽  
Molecular Mobility ◽  
Culture System

Molecular mobility of polyrotaxane surfaces promoted mineralization in a co-culture system of mesenchymal stem cells and endothelial cells.

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