scholarly journals Nitric Oxide-Induced Murine Hematopoietic Stem Cell Fate Involves Multiple Signaling Proteins, Gene Expression, and Redox Modulation

Stem Cells ◽  
2014 ◽  
Vol 32 (11) ◽  
pp. 2949-2960 ◽  
Author(s):  
Amanda Nogueira-Pedro ◽  
Carolina C. Dias ◽  
Helena Regina ◽  
C. Segreto ◽  
Priscilla C. Addios ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Stem Cell ◽  
Cell Fate ◽  
Signaling Proteins ◽  
Redox Modulation ◽  
Hematopoietic Stem ◽  
Stem Cell Fate ◽  
Murine Hematopoietic Stem Cell ◽  
Multiple Signaling

Signal control of hematopoietic stem cell fate: Wnt, Notch, and Hedgehog as the usual suspects

2008 ◽  
Vol 15 (4) ◽  
pp. 319-325 ◽  
Author(s):  
Clint Campbell ◽  
Ruth M Risueno ◽  
Simona Salati ◽  
Borhane Guezguez ◽  
Mickie Bhatia
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Hematopoietic Stem Cell ◽  
Signal Control ◽  
Hematopoietic Stem ◽  
Stem Cell Fate

Asymmetric organelle inheritance predicts human blood stem cell fate

Blood ◽  
2021 ◽  
Author(s):  
Dirk Loeffler ◽  
Florin Schneiter ◽  
Weijia Wang ◽  
Arne Wehling ◽  
Tobias Kull ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Division ◽  
Cell Fate ◽  
Human Blood ◽  
Asymmetric Cell Division ◽  
Cell Fates ◽  
Hematopoietic Stem ◽  
Stem Cell Fate ◽  
Blood Stem Cells

Understanding human hematopoietic stem cell fate control is important for their improved therapeutic manipulation. Asymmetric cell division, the asymmetric inheritance of factors during division instructing future daughter cell fates, was recently described in mouse blood stem cells. In human blood stem cells, the possible existence of asymmetric cell division remained unclear due to technical challenges in its direct observation. Here, we use long-term quantitative single-cell imaging to show that lysosomes and active mitochondria are asymmetrically inherited in human blood stem cells and that their inheritance is a coordinated, non-random process. Furthermore, multiple additional organelles, including autophagosomes, mitophagosomes, autolysosomes and recycling endosomes show preferential asymmetric co-segregation with lysosomes. Importantly, asymmetric lysosomal inheritance predicts future asymmetric daughter cell cycle length, differentiation and stem cell marker expression, while asymmetric inheritance of active mitochondria correlates with daughter metabolic activity. Hence, human hematopoietic stem cell fates are regulated by asymmetric cell division, with both mechanistic evolutionary conservation and differences to the mouse system.

Embryonic lineage tracing with Procr-CreER marks balanced hematopoietic stem cell fate during entire mouse lifespan

2019 ◽  
Vol 46 (10) ◽  
pp. 489-498 ◽  
Author(s):  
Xiaona Zheng ◽  
Guangyu Zhang ◽  
Yandong Gong ◽  
Xiaowei Ning ◽  
Zhijie Bai ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Hematopoietic Stem Cell ◽  
Lineage Tracing ◽  
Hematopoietic Stem ◽  
Stem Cell Fate

The PRO-inflammatory cytokine interleukin-1 is a key regulator of hematopoietic Stem cell fate and function

2016 ◽  
Vol 44 (9) ◽  
pp. S49
Author(s):  
Eric Pietras ◽  
Cristina Mirantes-Barbeito ◽  
Sarah Fong ◽  
Dirk Loeffler ◽  
Larisa Kovtonyuk ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Hematopoietic Stem Cell ◽  
Inflammatory Cytokine ◽  
Interleukin 1 ◽  
Hematopoietic Stem ◽  
Stem Cell Fate ◽  
And Function

scholarly journals Development of an Inexpensive Raman-Compatible Substrate for the Construction of a Microarray Screening Platform

2020 ◽  
Author(s):  
Isamar Pastrana-Otero ◽  
Sayani Majumdar ◽  
Aidan E. Gilchrist ◽  
Brittney L. Gorman ◽  
Brendan A. C. Harley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Fate ◽  
Living Cells ◽  
Raman Microspectroscopy ◽  
Partial Least Square ◽  
Hematopoietic Stem ◽  
Stem Cell Fate ◽  
Cell Fate Decisions ◽  
Extrinsic Cues

Biomaterial microarrays are being developed to facilitate identifying the extrinsic cues that elicit stem cell fate decisions to self-renew, differentiate and remain quiescent. Raman microspectroscopy, often combined with multivariate analysis techniques such as partial least square-discriminant analysis (PLS-DA), could enable the non-invasive identification of stem cell fate decisions made in response to extrinsic cues presented at specific locations on these microarrays. Because existing biomaterial microarrays are not compatible with Raman microspectroscopy, here, we develop an inexpensive substrate that is compatible with both single-cell Raman spectroscopy and the chemistries that are often used for biomaterial microarray fabrication. Standard deposition techniques were used to fabricate a custom Raman-compatible substrate that supports microarray construction. We validated that spectra from living cells on functionalized polyacrylamide (PA) gels attached to the custom Raman-compatible substrate are comparable to spectra acquired from a more expensive commercially available substrate. We also showed that the spectra acquired from individual living cells on functionalized PA gels attached to our custom substrates were of sufficient quality to enable accurate identification of cell phenotypes using PLS-DA models of the cell spectra. We demonstrated this by using cells from laboratory lines (CHO and transfected CHO cells) as well as adult stem cells that were freshly isolated from mice (long-term and short-term hematopoietic stem cells). The custom Ramancompatible substrate reported herein may be used as an inexpensive substrate for constructing biomaterial microarrays that enable the use of Raman microspectroscopy to non-invasively identify the fate decisions of stem cells in response to extrinsic cues.

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