scholarly journals B-Cell Receptor-Associated Protein 31 Regulates Human Embryonic Stem Cell Adhesion, Stemness, and Survival via Control of Epithelial Cell Adhesion Molecule

Stem Cells ◽  
2014 ◽  
Vol 32 (10) ◽  
pp. 2626-2641 ◽  
Author(s):  
Won-Tae Kim ◽  
Hong Seo Choi ◽  
Hyun Min Lee ◽  
Young-Joo Jang ◽  
Chun Jeih Ryu
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Human Embryonic Stem Cell ◽  
Cell Adhesion Molecule ◽  
Embryonic Stem ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Epithelial Cell Adhesion Molecule ◽  
Receptor Associated Protein

scholarly journals RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule

2011 ◽  
Vol 102 (5) ◽  
pp. 991-998 ◽  
Author(s):  
Sarah Shigdar ◽  
Jia Lin ◽  
Yan Yu ◽  
Mile Pastuovic ◽  
Ming Wei ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Cancer Stem Cell ◽  
Cell Adhesion Molecule ◽  
Stem Cell Marker ◽  
Rna Aptamer ◽  
Cancer Stem Cell Marker ◽  
Cell Marker ◽  
Epithelial Cell Adhesion Molecule

scholarly journals Solubility assessment of single-chain antibody fragment against epithelial cell adhesion molecule extracellular domain in four Escherichia coli strains

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Fatemeh Sadat Javadian ◽  
Majid Basafa ◽  
Aidin Behravan ◽  
Atieh Hashemi
Keyword(s):  
Escherichia Coli ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Extracellular Domain ◽  
Epithelial Cell Adhesion Molecule ◽  
Single Chain ◽  
E Coli ◽  
The Impact

Abstract Background Overexpression of the EpCAM (epithelial cell adhesion molecule) in malignancies makes it an attractive target for passive immunotherapy in a wide range of carcinomas. In comparison with full-length antibodies, due to the small size, the scFvs (single-chain variable fragments) are more suitable for recombinant expression in E. coli (Escherichia coli). However, the proteins expressed in large amounts in E. coli tend to form inclusion bodies that need to be refolded which may result in poor recovery of bioactive proteins. Various engineered strains were shown to be able to alleviate the insolubility problem. Here, we studied the impact of four E. coli strains on the soluble level of anti-EpEX-scFv (anti-EpCAM extracellular domain-scFv) protein. Results Although results showed that the amount of soluble anti-EpEX-scFv obtained in BL21TM (DE3) (114.22 ± 3.47 mg/L) was significantly higher to those produced in the same condition in E. coli RosettaTM (DE3) (71.39 ± 0.31 mg/L), and OrigamiTM T7 (58.99 ± 0.44 mg/L) strains, it was not significantly different from that produced by E. coli SHuffleTM T7 (108.87 ± 2.71 mg/L). Furthermore, the highest volumetric productivity of protein reached 318.29 ± 26.38 mg/L in BL21TM (DE3). Conclusions Although BL21TM (DE3) can be a suitable strain for high-level production of anti-EpEX-scFv protein, due to higher solubility yield (about 55%), E. coli SHuffleTM T7 seems to be better candidate for soluble production of scfv compared to BL21TM (DE3) (solubility yield of about 30%).

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