Leukemia Inhibitory Factor Induces In Vivo Expansion of Bone Marrow Progenitor Cells that Accelerate Hematopoietic Reconstitution but Do Not Enhance Radioprotection in Lethally Irradiated Mice

Stem Cells ◽  
1997 ◽  
Vol 15 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Johannes F. M. Pruijt ◽  
Ivan J. D. Lindley ◽  
Diana P. M. Heemskerk ◽  
Roel Willemze ◽  
Willem E. Fibbe
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Leukemia Inhibitory Factor ◽  
Inhibitory Factor ◽  
Bone Marrow Progenitor Cells ◽  
Bone Marrow Progenitor ◽  
Hematopoietic Reconstitution

scholarly journals Hypercholesterolemia suppresses Kir channels in porcine bone marrow progenitor cells in vivo

2007 ◽  
Vol 358 (1) ◽  
pp. 317-324 ◽  
Author(s):  
Emile R. Mohler ◽  
Yun Fang ◽  
Rebecca Gusic Shaffer ◽  
Jonni Moore ◽  
Robert L. Wilensky ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Kir Channels ◽  
Bone Marrow Progenitor Cells ◽  
Bone Marrow Progenitor

SDF-1 involvement in endothelial phenotype and ischemia-induced recruitment of bone marrow progenitor cells

Blood ◽  
2004 ◽  
Vol 104 (12) ◽  
pp. 3472-3482 ◽  
Author(s):  
Elena De Falco ◽  
Daniele Porcelli ◽  
Anna Rita Torella ◽  
Stefania Straino ◽  
Maria Grazia Iachininoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Time Course ◽  
Limb Ischemia ◽  
Artery Dissection ◽  
Hematopoietic Stem ◽  
Bone Marrow Progenitor Cells ◽  
Bone Marrow Progenitor

Chemokine stromal derived factor 1 (SDF-1) is involved in trafficking of hematopoietic stem cells (HSCs) from the bone marrow (BM) to peripheral blood (PB) and has been found to enhance postischemia angiogenesis. This study was aimed at investigating whether SDF-1 plays a role in differentiation of BM-derived c-kit+ stem cells into endothelial progenitor cells (EPCs) and in ischemia-induced trafficking of stem cells from PB to ischemic tissues. We found that SDF-1 enhanced EPC number by promoting α2, α4, and α5 integrin–mediated adhesion to fibronectin and collagen I. EPC differentiation was reduced in mitogen-stimulated c-kit+ cells, while cytokine withdrawal or the overexpression of the cyclin–dependent kinase (CDK) inhibitor p16INK4 restored such differentiation, suggesting a link between control of cell cycle and EPC differentiation. We also analyzed the time course of SDF-1 expression in a mouse model of hind-limb ischemia. Shortly after femoral artery dissection, plasma SDF-1 levels were up-regulated, while SDF-1 expression in the bone marrow was down-regulated in a timely fashion with the increase in the percentage of PB progenitor cells. An increase in ischemic tissue expression of SDF-1 at RNA and protein level was also observed. Finally, using an in vivo assay such as injection of matrigel plugs, we found that SDF-1 improves formation of tubulelike structures by coinjected c-kit+ cells. Our findings unravel a function for SDF-1 in increase of EPC number and formation of vascular structures by bone marrow progenitor cells.

scholarly journals Leukemia inhibitory factor/human interleukin for DA cells: a growth factor that stimulates the in vitro development of multipotential human hematopoietic progenitors

Blood ◽  
1991 ◽  
Vol 77 (2) ◽  
pp. 263-270 ◽  
Author(s):  
C Verfaillie ◽  
P McGlave
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Leukemia Inhibitory Factor ◽  
Colony Growth ◽  
Inhibitory Factor ◽  
Hematopoietic Progenitor ◽  
Bone Marrow Progenitor ◽  
Hla Dr ◽  
Eosinophil Colonies

Abstract We investigated the in vitro hematopoietic stimulatory activity of leukemia inhibitory factor/human interleukin for DA cells (LIF/HILDA) on bone marrow progenitor populations in 17 normal individuals. In serum-free cultures LIF/HILDA did not induce colony growth. In serum containing media, LIF/HILDA stimulated the growth of colony forming unit (CFU)-MIX and CFU-EO in a dose-dependent fashion and resulted in an increased CFU-MIX and burst forming unit-erythrocytes (BFU-E) colony size. Similar stimulatory effects were seen on a highly purified hematopoietic progenitor population obtained after immunomagnetic depletion of mature myeloid precursors and lymphoid cells. Addition of LIF/HILDA to cultures containing maximally stimulatory concentrations of recombinant human interleukin-3 (rhuIL3), rhuIL3 + rhuIL6, or rhu granulocyte-macrophage colony-stimulating factor (rhu GM-CSF) in serum containing media significantly increased the number of CFU-MIX and eosinophil colonies and increased size and cluster number of CFU-MIX and BFU-E. Depletion of accessory T lymphocytes or monocytes from bone marrow progenitors did not alter the response of hematopoietic precursors to LIF/HILDA. A similar increased colony growth was seen when LIF/HILDA was added to cultures of positively selected CD34/HLA- DR+ or CD34+/HLA-DR- bone marrow hematopoietic progenitor cells stimulated with maximally stimulatory concentrations of rhuIL3 + rhuIL6. LIF/HILDA is a novel cytokine capable of stimulating growth and proliferation of multi-lineage, erythroid, and eosinophil colonies in the presence of serum. LIF/HILDA exerts its activity by direct interaction with highly purified immature bone marrow progenitor cells, has an additive effect when used with other cytokines known to stimulate primitive hematopoietic precursors, and does not require accessory cells.

scholarly journals Leukemia inhibitory factor/human interleukin for DA cells: a growth factor that stimulates the in vitro development of multipotential human hematopoietic progenitors

Blood ◽  
1991 ◽  
Vol 77 (2) ◽  
pp. 263-270 ◽  
Author(s):  
C Verfaillie ◽  
P McGlave
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Leukemia Inhibitory Factor ◽  
Colony Growth ◽  
Inhibitory Factor ◽  
Hematopoietic Progenitor ◽  
Bone Marrow Progenitor ◽  
Hla Dr ◽  
Eosinophil Colonies

We investigated the in vitro hematopoietic stimulatory activity of leukemia inhibitory factor/human interleukin for DA cells (LIF/HILDA) on bone marrow progenitor populations in 17 normal individuals. In serum-free cultures LIF/HILDA did not induce colony growth. In serum containing media, LIF/HILDA stimulated the growth of colony forming unit (CFU)-MIX and CFU-EO in a dose-dependent fashion and resulted in an increased CFU-MIX and burst forming unit-erythrocytes (BFU-E) colony size. Similar stimulatory effects were seen on a highly purified hematopoietic progenitor population obtained after immunomagnetic depletion of mature myeloid precursors and lymphoid cells. Addition of LIF/HILDA to cultures containing maximally stimulatory concentrations of recombinant human interleukin-3 (rhuIL3), rhuIL3 + rhuIL6, or rhu granulocyte-macrophage colony-stimulating factor (rhu GM-CSF) in serum containing media significantly increased the number of CFU-MIX and eosinophil colonies and increased size and cluster number of CFU-MIX and BFU-E. Depletion of accessory T lymphocytes or monocytes from bone marrow progenitors did not alter the response of hematopoietic precursors to LIF/HILDA. A similar increased colony growth was seen when LIF/HILDA was added to cultures of positively selected CD34/HLA- DR+ or CD34+/HLA-DR- bone marrow hematopoietic progenitor cells stimulated with maximally stimulatory concentrations of rhuIL3 + rhuIL6. LIF/HILDA is a novel cytokine capable of stimulating growth and proliferation of multi-lineage, erythroid, and eosinophil colonies in the presence of serum. LIF/HILDA exerts its activity by direct interaction with highly purified immature bone marrow progenitor cells, has an additive effect when used with other cytokines known to stimulate primitive hematopoietic precursors, and does not require accessory cells.

scholarly journals CD34/CD133 enriched bone marrow progenitor cells promote neovascularization of tissue engineered constructs in vivo

2014 ◽  
Vol 13 (3) ◽  
pp. 465-477 ◽  
Author(s):  
Marietta Herrmann ◽  
Andreas Binder ◽  
Ursula Menzel ◽  
Stephan Zeiter ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Progenitor Cells ◽  
Bone Marrow Progenitor
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