scholarly journals Ceramide-1-Phosphate Regulates Migration of Multipotent Stromal Cells and Endothelial Progenitor Cells-Implications for Tissue Regeneration

Stem Cells ◽  
2013 ◽  
Vol 31 (3) ◽  
pp. 500-510 ◽  
Author(s):  
Chihwa Kim ◽  
Gabriela Schneider ◽  
Ahmed Abdel-Latif ◽  
Kasia Mierzejewska ◽  
Manjula Sunkara ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Tissue Regeneration ◽  
Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Endothelial Progenitor ◽  
Ceramide 1

scholarly journals Syndecan-4 Functionalization of Tissue Regeneration Scaffolds Improves Interaction with Endothelial Progenitor Cells

2021 ◽  
Author(s):  
Harleigh Warner ◽  
Yidi Wu ◽  
William D Wagner
Keyword(s):  
Stem Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Tissue Regeneration ◽  
Binding Sites ◽  
Type I Collagen ◽  
Type I ◽  
Endothelial Progenitor ◽  
Hybrid Scaffold ◽  
Scaffold Materials

Abstract Key to most implanted cell free scaffolds for tissue regeneration is the ability to sequester and retain undifferentiated mesenchymal stem cells at the repair site. In this report, syndecan-4, a heparan sulfate containing proteoglycan, was investigated as a unique molecule for use in scaffold functionalization. An electrospun hybrid scaffold comprised of poly (glycerol) sebacate (PGS), silk fibroin and type I collagen (PFC) was used as a model scaffold to develop a procedure and test the hypothesis that functionalization would result in increased scaffold binding of endothelial progenitor cells (EPCs). For these studies both Syndecan-4 and stromal derived factor-1α (SDF-1α) were used in functionalization PFC. Syndecan-4 functionalized PFC bound 4.8 fold more SDF-1α compared to nonfunctionalized PFC. Binding was specific as determined by heparin displacement studies. After culture for 7 days, significantly, more EPCs were detected on PFC scaffolds having both syndecan-4 and SDF-1α compared to scaffolds of PFC with only syndecan-4, or PFC adsorbed with SDF-1α, or PFC alone. Taken together, this study demonstrates that EPCs can be bound to and significantly expanded on PFC material through syndecan-4 mediated growth factor binding. Syndecan-4 with a multiplicity of binding sites has the potential to functionalize and expand stem cells on a variety of scaffold materials for use in tissue regeneration.

scholarly journals Rejuvenation of Senescent Endothelial Progenitor Cells by Extracellular Vesicles Derived From Mesenchymal Stromal Cells

2020 ◽  
Vol 5 (11) ◽  
pp. 1127-1141
Author(s):  
Liyong Wang ◽  
Jianqing Wei ◽  
Andrea Da Fonseca Ferreira ◽  
Huilan Wang ◽  
Lukun Zhang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Endothelial Progenitor
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