scholarly journals X-Ray-Visible Microcapsules Containing Mesenchymal Stem Cells Improve Hind Limb Perfusion in a Rabbit Model of Peripheral Arterial Disease

Stem Cells ◽  
2012 ◽  
Vol 30 (6) ◽  
pp. 1286-1296 ◽  
Author(s):  
Dorota A. Kedziorek ◽  
Lawrence V. Hofmann ◽  
Yingli Fu ◽  
Wesley D. Gilson ◽  
Kenyatta M. Cosby ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Peripheral Arterial Disease ◽  
Hind Limb ◽  
Rabbit Model ◽  
Arterial Disease ◽  
X Ray ◽  
Limb Perfusion ◽  
Peripheral Arterial

Abstract 5281: Bioluminescence Imaging of X-Ray Visible Microencapsulated Mesenchymal Stem Cells for Cell Based Therapy of Peripheral Arterial Disease

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Dorota A Kedziorek ◽  
Piotr Walczak ◽  
Yingli Fu ◽  
Nicole Azene ◽  
Aravind Arepally ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Peripheral Arterial Disease ◽  
Arterial Disease ◽  
X Ray ◽  
Encapsulated Cells ◽  
X Ray Imaging ◽  
Peripheral Arterial

Introduction: Therapeutic angiogenesis in Peripheral Arterial Disease (PAD) using stem cell therapy is potentially complicated by immunorejection. To overcome this problem, microen-capsulation using the alginate-poly-L-lysine (PLL)-alginate (APA) method was developed to provide a protective porous bubble to block antibodies but allow exchange of small molecules. Recently, we have developed a method to enable X-ray detection of these capsules. However, cell survival within the capsules could not be determined. Plus PLL can be mildly cytotoxic. In the present study, we combined reporter gene methods to verify cell survival with X-ray detection of the microcapsules in a rabbit PAD model and studied the PLL impact on cell viability. Methods: Rabbit mesenchymal stem cells (MSCs) were transfected with triple fusion (TF) reporter gene for bioluminescence (firefly luciferase), fluorescence (red fluorescent protein) and PET (truncated thymidine kinase). TF-MSCs were encapsulated in the perfluorooctyl bromide (PFOB) capsules to enable computed tomographic detection. Capsule crosslinking was performed with three PLL concentrations, i.e., 0.005%, 0.025% and 0.05%. Bioluminescent imaging (BLI) was used to monitor cells survival for one week in vitro and after intramuscular injection in vivo . Results: Serial in vitro BLI enabled the detection of viable encapsulated MSCs without detrimental signal degradation (~13% decrease of BLI signal intensity after PFOB encapsulation comparing to equal number of naked MSCs). PLL did not result in cell death; higher PLL concentrations were correlated with stronger BLI signal. BLI signal production was only slightly reduced by second layer of alginate (~80% for 0.05% PLL). In vivo BLI demonstrated the detection of naked, APA, and PFOB-encapsulated TF-MSCs. X-ray imaging enabled PFOB microcapsules detection relative to vasculature. Conclusion: BLI allows monitoring of encapsulated cells survival. PLL concentrations ≤ 0.05% appear safe for encapsulated cells with higher concentration being associated with enhanced crosslinking and capsule stability. MSCs expressing TF reporter in PFOB microcapsules enables dual monitoring of cell delivery/capsule tracking by X-ray imaging and cell viability with BLI.

scholarly journals Reversible secretome and signaling defects in diabetic mesenchymal stem cells from peripheral arterial disease patients

2018 ◽  
Vol 68 (6) ◽  
pp. 137S-151S.e2 ◽  
Author(s):  
Tatiana Chadid ◽  
Andrew Morris ◽  
Alexandra Surowiec ◽  
Scott Robinson ◽  
Maiko Sasaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Peripheral Arterial Disease ◽  
Arterial Disease ◽  
Peripheral Arterial

scholarly journals Intra-Arterial Delivery of Mesenchymal Stem Cells for Regenerative Medicine to Treat Peripheral Arterial Disease

2021 ◽  
Vol 74 (3) ◽  
pp. e297-e298
Author(s):  
Mira Shoukry ◽  
Samuel Nussbaum ◽  
Karen Jeong ◽  
Ralph Perkerson ◽  
Takahisa Kanekiyo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
Peripheral Arterial Disease ◽  
Arterial Disease ◽  
Peripheral Arterial

scholarly journals NOVEL 19F MRI AND CT TRACKABLE MICROENCAPSULATED MESENCHYMAL STEM CELLS FOR TREATING PERIPHERAL ARTERIAL DISEASE

2010 ◽  
Vol 55 (10) ◽  
pp. A216.E2049 ◽  
Author(s):  
Yingli Fu ◽  
Dorota Kedziorek ◽  
Steven Shea ◽  
Ronald Ouwerkerk ◽  
Gary Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Peripheral Arterial Disease ◽  
Arterial Disease ◽  
19F Mri ◽  
Peripheral Arterial

Abstract No. 176: Encapsulated X-Ray Visible Stem Cells for Arteriogenic Therapy in Peripheral Arterial Disease

2008 ◽  
Vol 19 (2) ◽  
pp. S67 ◽  
Author(s):  
D.L. Kraitchman ◽  
D.A. Kedziorek ◽  
W.D. Gilson ◽  
K. Cosby ◽  
G. Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Peripheral Arterial Disease ◽  
Arterial Disease ◽  
X Ray ◽  
Peripheral Arterial

Abdominal Aortic Calcifications Predict Survival in Peritoneal Dialysis Patients

2018 ◽  
Vol 38 (5) ◽  
pp. 366-373 ◽  
Author(s):  
Satu Mäkelä ◽  
Markku Asola ◽  
Henrik Hadimeri ◽  
James Heaf ◽  
Maija Heiro ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Peripheral Arterial Disease ◽  
Ankle Brachial Index ◽  
Arterial Disease ◽  
Aortic Calcification ◽  
Dialysis Patients ◽  
X Ray ◽  
Abdominal Aortic ◽  
All Cause Mortality ◽  
Peripheral Arterial

BackgroundPeripheral arterial disease and vascular calcifications contribute significantly to the outcome of dialysis patients. The aim of this study was to evaluate the prognostic role of severity of abdominal aortic calcifications and peripheral arterial disease on outcome of peritoneal dialysis (PD) patients using methods easily available in everyday clinical practice.MethodsWe enrolled 249 PD patients (mean age 61 years, 67% male) in this prospective, observational, multicenter study from 2009 to 2013. The abdominal aortic calcification score (AACS) was assessed using lateral lumbar X ray, and the ankle-brachial index (ABI) using a Doppler device.ResultsThe median AACS was 11 (range 0 – 24). In 58% of the patients, all 4 segments of the abdominal aorta showed deposits, while 19% of patients had no visible deposits (AACS 0). Ankle-brachial index was normal in 49%, low (< 0.9) in 17%, and high (> 1.3) in 34% of patients. Altogether 91 patients (37%) died during the median follow-up of 46 months. Only 2 patients (5%) with AACS 0 died compared with 50% of the patients with AACS ≥ 7 ( p < 0.001). The adjusted hazard ratio for all-cause mortality was 4.85 (95% confidence interval [CI] 1.94 – 24.46) for aortic calcification (AACS ≥ 7), 2.14 for diabetes (yes/no), 0.93 for albumin (per 1 g/L), and 1.04 for age (per year). A low or high ABI were not independently associated with mortality.ConclusionsSevere aortic calcification was a strong predictor of all-cause mortality in PD patients. The evaluation of aortic calcifications by lateral X ray is a simple method that allows the identification of high-risk patients.

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