Characterization of Starch from Two Andean Potatoes: Ccompis (Solanum tuberosum spp. andigena ) and Huayro (Solanum x chaucha )

2017 ◽  
Vol 70 (3-4) ◽  
pp. 1700134 ◽  
Author(s):  
Fausto H. Cisneros ◽  
Roberto Zevillanos ◽  
Mariella Figueroa ◽  
Gabriela Gonzalez ◽  
Luis Cisneros-Zevallos
Planta ◽  
2007 ◽  
Vol 227 (2) ◽  
pp. 387-396 ◽  
Author(s):  
Zhihong Lang ◽  
Peng Zhou ◽  
Jingjuan Yu ◽  
Guangming Ao ◽  
Qian Zhao

2003 ◽  
Vol 23 (11) ◽  
pp. 4000-4012 ◽  
Author(s):  
Ludovic Delage ◽  
André Dietrich ◽  
Anne Cosset ◽  
Laurence Maréchal-Drouard

ABSTRACT Some of the mitochondrial tRNAs of higher plants are nuclearly encoded and imported into mitochondria. The import of tRNAs encoded in the nucleus has been shown to be essential for proper protein translation within mitochondria of a variety of organisms. Here, we report the development of an in vitro assay for import of nuclearly encoded tRNAs into plant mitochondria. This in vitro system utilizes isolated mitochondria from Solanum tuberosum and synthetic tRNAs transcribed from cloned nuclear tRNA genes. Although incubation of radioactively labeled in vitro-transcribed tRNAAla, tRNAPhe, and tRNAMet-e with isolated potato mitochondria resulted in importation, as measured by nuclease protection, the amount of tRNA transcripts protected at saturation was at least five times higher for tRNAAla than for the two other tRNAs. This difference in in vitro saturation levels of import is consistent with the in vivo localization of these tRNAs, since cytosolic tRNAAla is naturally imported into potato mitochondria whereas tRNAPhe and tRNAMet-e are not. Characterization of in vitro tRNA import requirements indicates that mitochondrial tRNA import proceeds in the absence of any added cytosolic protein fraction, involves at least one protein component on the surface of mitochondria, and requires ATP-dependent step(s) and a membrane potential.


Planta ◽  
1998 ◽  
Vol 207 (2) ◽  
pp. 181-188 ◽  
Author(s):  
María José Carmona ◽  
Nely Ortega ◽  
Federico Garcia-Maroto

Planta ◽  
2009 ◽  
Vol 230 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Silvia Marina País ◽  
Marina Alejandra González ◽  
María Teresa Téllez-Iñón ◽  
Daniela Andrea Capiati

2019 ◽  
Vol 13 (1) ◽  
pp. 44-49
Author(s):  
Carmen Rojas-Padi ◽  
Victor Vasquez-Vi ◽  
Viviano Ninaquispe ◽  
Julio Cesar Rojas-Nacc ◽  
Nelson Rios-Campo ◽  
...  

2016 ◽  
Vol 34 (3) ◽  
pp. 317-328 ◽  
Author(s):  
Pablo Gutiérrez S. ◽  
Mauricio Marín M. ◽  
Daniel Muñoz E.

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.


1993 ◽  
Vol 102 (3) ◽  
pp. 1043-1043 ◽  
Author(s):  
S. B. Smith ◽  
M. A. Taylor ◽  
L. R. Burch ◽  
H. V. Davies

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