A Rapid In-vitro Digestibility Assay Based on Glucometry for Investigating Kinetics of Starch Digestion

2009 ◽  
Vol 61 (5) ◽  
pp. 245-255 ◽  
Author(s):  
Peter A. Sopade ◽  
Michael J. Gidley
Keyword(s):  
In Vitro Digestibility ◽  
Starch Digestion ◽  
Kinetics Of

In vitro starch digestion kinetics of diets varying in resistant starch and arabinoxylan compared with in vivo portal appearance of glucose in pigs

2016 ◽  
Vol 88 ◽  
pp. 199-206 ◽  
Author(s):  
Cecilie Toft Vangsøe ◽  
Anne Krog Ingerslev ◽  
Peter Kappel Theil ◽  
Mette Skou Hedemann ◽  
Helle Nygaard Lærke ◽  
...  
Keyword(s):  
Resistant Starch ◽  
Starch Digestion ◽  
Digestion Kinetics ◽  
Kinetics Of

scholarly journals 133 Porcine in vitro degradation and fermentation characteristics of heat-pretreated and multi-enzyme-supplemented soybean hulls

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 114-115
Author(s):  
Cienna J Boss ◽  
Jung Wook Lee ◽  
Rob Patterson ◽  
Tofuko A Woyengo
Keyword(s):  
In Vitro Digestion ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Soybean Hulls ◽  
In Vitro Fermentation ◽  
Heat Pretreatment ◽  
Fractional Rate ◽  
Enzyme Supplementation ◽  
Kinetics Of

Abstract A study was conducted to determine effects of pretreating and supplementing soybean hulls with multi-enzyme on porcine in vitro digestion and fermentation characteristics. Treatments were untreated and heat-pretreated (160 °C and 70 psi for 20 min) soybean hulls without or with multi-enzyme in a 2 × 2 factorial arrangement. The multi-enzyme supplied 2,800 U of cellulase, 1,800 U of pectinase, 400 U of mannanase, 1,000 U of xylanase, 600 U of glucanase, and 200 U of protease/kilogram of feedstuff. Feedstuffs were subjected to in vitro digestion with porcine pepsin and pancreatin, followed by in vitro fermentation for 72 h. Accumulated gas production was recorded and modeled to estimate kinetics of gas production. On DM basis, untreated and pretreated soybean hulls contained 10.4 and 10.6% CP, and 63.2 and 49.5% ADF, respectively. Pretreatment and multi-enzyme supplementation did not interact on in vitro digestibility of DM (IVDDM). Untreated and pretreated soybean hulls did not differ in IVDDM (24.8 vs. 25.7%). Multi-enzyme increased (P < 0.05) IVDDM of soybean hulls by a mean of 45.5%. Pretreatment and multi-enzyme unaffected total gas production. Pretreatment and multi-enzyme interacted (P < 0.05) on fractional rate of degradation such that the fractional rate of degradation for pretreated soybean hulls was greater (P < 0.05) than that of untreated soybean hulls when soybean hulls were supplemented with multi-enzyme (0.045 vs. 0.062 h-1), but not when soybean hulls were unsupplemented with multi-enzyme (0.053 vs. 0.059 h-1). In conclusion, multi-enzyme supplementation increased IVDDM, implying that the multi-enzyme used in the study can be used to enhance utilization of soybean hulls. Heat pretreatment increased the rate of fermentation of multi-enzyme-supplemented soybean hulls, implying that the rate of fermentation of soybean hulls in the hindgut of pigs can be enhanced by a combination of heat pretreatment and multi-enzyme supplementation.

In vitro rumen gas production kinetics, hydrocyanic acid concentration and fermentation characteristics of fresh cassava root and feed block sulfur concentration

2020 ◽  
Vol 60 (5) ◽  
pp. 659 ◽  
Author(s):  
Gamonmas Dagaew ◽  
Anusorn Cherdthong ◽  
Metha Wanapat ◽  
Pin Chanjula
Keyword(s):  
Nitrogen Concentration ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Hydrocyanic Acid ◽  
In Vitro Digestibility ◽  
High Concentration ◽  
Cassava Root ◽  
Fermentation Liquor ◽  
Kinetics Of

Context Feeding ruminants with fresh cassava roots (FCR) is limited because they have a high concentration of hydrocyanic acid (HCN). Thus, it was hypothesised that receiving a feed block containing high sulfur (FBS) would reduce hydrocyanic acid (HCN) in FCR and improve rumen fermentation and nutrient digestibility in animals. Aims The goal of the present work was to study the influence of the ratio of FCR to rice straw (RS) together with FBS on kinetics of gas production, HCN concentration, fermentation characteristics and nutrient digestibility, using in vitro technique. Methods The experimental design was a 4 × 2 factorial arrangement in a completely randomised design, with three replications per treatment. Factor A was the FCR to RS ratio, which was 100:0, 60:40, 40:60 or 0:100. Factor B was sulfur, which was provided as two concentrations in FBS (2% and 4% DM). Gas production was recorded after incubation, at 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72 and 96 h. Fermentation liquor was collected and determined for kinetics of gas production, HCN concentration, fermentation characteristics and nutrient digestibility. Key results Cassava root to RS ratio influenced the cumulative gas production after 96 h. Inclusion of sulfur in the FBS at 4% increased the cumulative gas production, when compared with inclusion at 2%. The gas production from degradable fraction (b) and the rate of gas production (c) were significantly different among the treatments with different FCR:RS ratios, whereas there was no difference between using 2% and 4% sulfur in the FBS. The HCN concentration in fermentation liquor increased with an increasing proportion of FCR. Furthermore, inclusion of sulfur in the feed block at 4% reduced HCN concentration by 42.8%, when compared with inclusion at 2% (P < 0.05). Ammonia-nitrogen concentration was significantly different among the FCR:RS-ratio treatments and was reduced when the proportion of FCR was decreased (P < 0.05). In vitro digestibility was significantly increased with an increasing proportion of FCR. Increasing the proportion of FCR with 4% of sulfur in the FBS significantly increased in vitro DM digestibility, compared with 2% sulfur. Increasing the FCR:RS ratio with 4% of sulfur in the FBS increased the proportion of propionic acid (P < 0.05). Conclusions Using a high FCR:RS ratio (100:0 or 60:40) with 4% sulfur in the FBS enhanced kinetics of gas production, propionic molar proportion, nutrient digestibility, and HCN detoxification by rumen microbes in an in vitro trial. Implications An in vivo study should be encouraged to verify the results and obtain more data.

In vitro digestibility kinetics of diets containing different cereal sources

2010 ◽  
Vol 134 (1-3) ◽  
pp. 47-49 ◽  
Author(s):  
A.M. Pérez-Vendrell ◽  
D. Torrallardona
Keyword(s):  
In Vitro Digestibility ◽  
Kinetics Of

scholarly journals Evaluation of Nutritive Values of Agro-Industrial By-Products (AIBPS) and Effect of Their Nano Structural Characteristics on the Kinetics of Biogas Production and Methane Emission

2021 ◽  
Author(s):  
Shahram Shirmohammadi ◽  
Akbar Taghizadeh ◽  
Hamid Paya ◽  
Arash Javanmard ◽  
Soheila Abachi ◽  
...  
Keyword(s):  
Methane Emission ◽  
Biogas Production ◽  
Ex Vivo ◽  
Structural Characteristics ◽  
In Vitro Digestibility ◽  
Fermentation Parameters ◽  
Experimental Treatments ◽  
Kinetics Of ◽  
By Products

Abstract Over the past decades, the agro-industrial by-products (AIBP) has received considerable attention. With this motivation, the aim of this study was to investigate the effect of AIBP as a source of non-fiber carbohydrates on biogas production kinetic, methane emission and fermentation characteristics. Experimental treatments were (1) Sugar beet pulp (SBP) (control), (2) Apple pomace (AP), (3) Orange pulp (OP, (4) 33% AP + 66% OP, (5) 50% AP + 50% OP and (6) 66% AP + 33%OP. For this work, we analyzed the data collected from the kinetics of digestion through biogas production, ex-vivo methane emission, in-vitro digestibility of dry matter and fermentation parameters. Field emission scanning electron microscope was used to show the nano structural differences of the AIBPs. Our results demonstrated the significant differences of the crude protein among the treatments (P < 0.05). Biogas production and methane emission were significantly higher in SBP and OP treatments (P < 0.05). The most eminent and the lowest amounts of acetate were observed for AP and OP (61.84 mmol/L, 58.15 mmol/L), respectively. More broken edges were obvious in OP images. particle size was rather smaller in SBP. Images of AP showed a sleek surface which may act as a shield preventing more digestion. Overall, beside reducing environmental contamination by AIBP, our results showed a positive effect of AIBPs on degradation and biogas kinetics, methane emission and in vitro fermentation parameters describing that they can be used as a good source of non-fiber energy sources.

Prediction of Digestibility and Intake of Sheep Fed Leguminosae or GRAMINAE Hays: Comparison Between the in Vitro Digestibility, Kinetics of Gas Production or Dry Matter Degradation

1994 ◽  
Vol 1994 ◽  
pp. 103-103
Author(s):  
M.T. Dentinho ◽  
K. Khazaal ◽  
J.M. Ribeiro ◽  
E.R. Ørskov
Keyword(s):  
Dry Matter ◽  
Lolium Multiflorum ◽  
Daily Intake ◽  
Gas Production ◽  
Italian Ryegrass ◽  
In Vitro Digestibility ◽  
Kinetics Of

By using separated values of kinetics of in situ dry matter (DM) degradation or in vitro gas production (Menke and Steingass, 1988) of leguminosae hays, Khazaal et al, (1993) reported high correlation with intake (r= 0.88; r= 0.79) and in vivo DM digestibility (DMD) (r= 0.94; r= 0.88). The aim of the present study was to extend the range of samples used and compare the ability of the 2 stages in vitro digestibility (Tilley and Terry, 1963), the in situ DM degradation or the gas production techniques to predict daily intake (g DM/ kgW0.75) and in vivo DM digestibility (DMD) of 19 leguminous and graminaceous hays fed to sheep.Three harvesting stages (early bloom EB, mid bloom MB or in seed IS) made from lucerne (Medicago sativa), sweet clover (Melilotus segetalis), Persian clover (Trifolium resupinatum), Rye (Secale cereale), Triticale (Triticale hexaploid), oat (Avena stativa) and a pre-bloom (PB) Italian ryegrass (Lolium multiflorum ). Each hay was fed ad libitum to 4 Merino male sheep and their intake and in vivo DMD recorded. Gas production (ml/ 200 mg DM) or in situ DM degradation (g/ 100 g DM) were determined as described by Khazaal et al, (1993) after 6, 12, 24, 48, 72 or 96 h incubation. Measured gas production or DM degradation values were fitted to the equation p=a+b(l-e-ct)(McDonald, 1981) where p is gas production or DM degradation at time t and a, b and c are constants. For nylon bag the washing loss (soluble fraction) was defined as A, the insoluble but fermentable matter was defined as B=(a+b)-A, and c is the rate of fermentation or degradation (Ørskov and Ryle, 1990).

scholarly journals Pre-incubation of ruminal inocula to assess in vitro gas production and digestibility

2020 ◽  
Vol 50 (8) ◽  
Author(s):  
Juan Manuel Cantet ◽  
Darío Colombatto ◽  
Marisa Wawrzkiewicz ◽  
Gustavo Jaurena
Keyword(s):  
Gas Production ◽  
In Vitro Digestibility ◽  
Fermentation Products ◽  
In Vitro Gas Production ◽  
Production Techniques ◽  
Ruminal Degradation ◽  
Fermentative Activity ◽  
Fermentation Parameters ◽  
Kinetics Of

ABSTRACT: In vitro gas production techniques represent a valuable tool to describe the kinetics of ruminal degradation of food. However, the ruminal liquor used as a microbial inoculum has been a great source of variation and error. A standardization of this factor should contribute to assure the independence of food fermentation parameters from those of the inocula. In this research it was hypothesized that a controlled pre-incubation treatment of ruminal liquor could contribute to stabilize and homogenize the undigested residues of blanks and as a consequence, of the production of residual cumulative gas production (CGP). A pre-incubation (i.e. previous real incubation) of rumen inocula was developed with a simple substrate similar to the diet offered to donors at 1% w/v for 0, 1, 2 and 4 h (Control, Prei-1, Prei-2 and Prei-4 treatments respectively). Once the pre-incubation hours were completed, they were incubated with contrasting substrates and without substrate (i.e. blanks) in order to evaluate the CGP, in vitro digestibility of the DM and fermentation products. Although, the fermentative activity of the pre-incubated inoculums worked satisfactorily in the in vitro system, contrary to what was speculated, residues of the pre-incubation increased the variability and heterogeneity of variances among blanks. Consequently, it was concluded that the pre-incubations did not work to generate more homogeneous and less variable ruminal liquor for the in vitro gas production system.

In vitro protein and starch digestion kinetics of individual chickpea cells: from static to more complex in vitro digestion approaches

2021 ◽  
Author(s):  
Katharina Pälchen ◽  
Daphne Michels ◽  
Dorine Duijsens ◽  
Shannon Tabeth Gwala ◽  
Andrea Katherine Pallares Pallares ◽  
...  
Keyword(s):  
In Vitro Digestion ◽  
Starch Digestion ◽  
Digestion Kinetics ◽  
Kinetics Of ◽  
Vitro Protein

Attention has been given to more (semi-)dynamic in vitro digestion approaches, ascertaining the consequences of dynamic in vivo aspects on in vitro digestion kinetics. As these often come with time...

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