scholarly journals Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label‐Free Super‐Resolution Microscopy

Small Methods ◽  
2021 ◽  
pp. 2000985
Author(s):  
Milan Vala ◽  
Łukasz Bujak ◽  
Antonio García Marín ◽  
Kristýna Holanová ◽  
Verena Henrichs ◽  
...  
Keyword(s):  
Super Resolution ◽  
Label Free ◽  
Structural Fluctuations ◽  
Super Resolution Microscopy

scholarly journals Front Cover: Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label‐Free Super‐Resolution Microscopy (Small Methods 4/2021)

Small Methods ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 2170012
Author(s):  
Milan Vala ◽  
Łukasz Bujak ◽  
Antonio García Marín ◽  
Kristýna Holanová ◽  
Verena Henrichs ◽  
...  
Keyword(s):  
Super Resolution ◽  
Label Free ◽  
Front Cover ◽  
Structural Fluctuations ◽  
Super Resolution Microscopy

scholarly journals Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy

2020 ◽  
Author(s):  
Milan Vala ◽  
Łukasz Bujak ◽  
Antonio García Marín ◽  
Kristýna Holanová ◽  
Verena Henrichs ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Electron Micrographs ◽  
Structural Changes ◽  
Structural Transition ◽  
Super Resolution ◽  
Label Free ◽  
Discrete Process ◽  
Static Electron ◽  
Structural Fluctuations ◽  
Super Resolution Microscopy

AbstractMicrotubules are cytoskeletal polymers of tubulin dimers assembled into protofilaments that constitute nanotubes undergoing periods of assembly and disassembly. Static electron micrographs suggest a structural transition of straight protofilaments into curved ones occurring at the tips of disassembling microtubules. However, these structural transitions have never been observed and the process of microtubule disassembly thus remains unclear. Here, a label-free optical microscopy capable of selective imaging of the transient structural changes of protofilaments at the tip of a disassembling microtubule is introduced. Upon induced disassembly, the transition of ordered protofilaments into a disordered conformation is resolved at the tip of the microtubule. Imaging the unbinding of individual tubulin oligomers from the microtubule tip reveals transient pauses and relapses in the disassembly, concurrent with enrichment of ordered protofilament segments at the microtubule tip. These findings show that microtubule disassembly is a discrete process and suggest a mechanism of switching from the disassembly to the assembly phase.

Resolving plasmonic hotspots by label-free super-resolution microscopy

2021 ◽  
Author(s):  
xue cheng ◽  
Congyue Liu ◽  
Guorui Zhang ◽  
Wei Liu ◽  
Ju wang ◽  
...  
Keyword(s):  
Super Resolution ◽  
Label Free ◽  
Super Resolution Microscopy

Applications of nanostructures in wide-field, label-free super resolution microscopy

2018 ◽  
Vol 27 (11) ◽  
pp. 118704 ◽  
Author(s):  
Xiaowei Liu ◽  
Chao Meng ◽  
Xuechu Xu ◽  
Mingwei Tang ◽  
Chenlei Pang ◽  
...  
Keyword(s):  
Super Resolution ◽  
Wide Field ◽  
Label Free ◽  
Super Resolution Microscopy

Toward Label-Free Super-Resolution Microscopy

ACS Photonics ◽  
2015 ◽  
Vol 3 (1) ◽  
pp. 79-86 ◽  
Author(s):  
W. Ruchira Silva ◽  
Christian T. Graefe ◽  
Renee R. Frontiera
Keyword(s):  
Super Resolution ◽  
Label Free ◽  
Super Resolution Microscopy

scholarly journals Label-Free Super-Resolution Microscopy By Means of Transient Absorption Saturation

2021 ◽  
Vol 120 (3) ◽  
pp. 181a
Author(s):  
Behjat Sadat Kariman ◽  
Takahiro Deguchi ◽  
Marco Scotto d'Abbusco ◽  
Giulia Zanini ◽  
Alberto Diaspro ◽  
...  
Keyword(s):  
Transient Absorption ◽  
Super Resolution ◽  
Label Free ◽  
Absorption Saturation ◽  
Super Resolution Microscopy

scholarly journals Label-free super-resolution imaging below 90-nm using photon-reassignment

2021 ◽  
Vol 1 ◽  
pp. 3
Author(s):  
Alberto Aguilar ◽  
Adeline Boyreau ◽  
Pierre Bon
Keyword(s):  
Biological Samples ◽  
Optical Resolution ◽  
Super Resolution ◽  
Cost Effective ◽  
Label Free ◽  
Cost Effective Approach ◽  
Imaging Mode ◽  
Resolution Imaging ◽  
Fluorescent Tags ◽  
Super Resolution Microscopy

Background: Achieving resolutions below 100 nm is key for many fields, including biology and nanomaterial characterization. Although nearfield and electron microscopy are the gold standards for studying the nanoscale, optical microscopy has seen its resolution drastically improve in the last decades. So-called super-resolution microscopy is generally based on fluorescence photophysics and requires modification of the sample at least by adding fluorescent tags, an inevitably invasive step. Therefore, it remains very challenging and rewarding to achieve optical resolutions beyond the diffraction limit in label-free samples. Methods: Here, we present a breakthrough to unlock label-free 3D super-resolution imaging of any object including living biological samples. It is based on optical photon-reassignment in confocal reflectance imaging mode. Results: We demonstrate that we surpass the resolution of all fluorescence-based confocal systems by a factor ~1.5. We have obtained images with a 3D (x,y,z) optical resolution of (86x86x248) nm3 using a visible wavelength (445 nm) and a regular microscope objective (NA=1.3). The results are presented on nanoparticles as well as on (living) biological samples. Conclusions: This cost-effective approach double the resolution of reflectance confocal microscope with minimal modifications. It is therefore compatible with any microscope and sample, works in real-time, and does not require any signal processing.

scholarly journals Structural Changes and Dynamics of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy

2021 ◽  
Vol 120 (3) ◽  
pp. 181a
Author(s):  
Milan Vala ◽  
Łukasz Bujak ◽  
Antonio G. Marin ◽  
Kristýna Holanová ◽  
Verena Henrichs ◽  
...  
Keyword(s):  
Structural Changes ◽  
Super Resolution ◽  
Label Free ◽  
Super Resolution Microscopy
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