scholarly journals A Cell for Controllable Formation and In Operando Electrochemical Characterization of Intercalation Materials for Aqueous Metal‐Ion Batteries

Small Methods ◽  
2019 ◽  
Vol 3 (12) ◽  
pp. 1900445 ◽  
Author(s):  
Philipp Marzak ◽  
Philipp Moser ◽  
Siegfried Schreier ◽  
Daniel Scieszka ◽  
Jeongsik Yun ◽  
...  
Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 963 ◽  
Author(s):  
Hanbeen Kim ◽  
Hyo Gun Lee ◽  
Inhyuk Kwon ◽  
Jakyeom Seo

Streptococcus bovis (S. bovis) is one of the critical initiators of acute acidosis in ruminants. Therefore, we aimed to develop and characterize the endolysin LyJH307, which can lyse ruminal S. bovis. We tested the bactericidal activity of recombinant LyJH307 against S. bovis JB1 under a range of pH, temperature, NaCl, and metal ion concentrations. In silico analyses showed that LyJH307 has a modular design with a distinct, enzymatically active domain of the NLPC/P60 superfamily at the N-terminal and a cell wall binding domain of the Zoocin A target recognition domain (Zoocin A_TRD) superfamily at the C-terminal. The lytic activity of LyJH307 against S. bovis JB1 was the highest at pH 5.5, and relatively higher under acidic, than under alkaline conditions. LyJH307 activity was also the highest at 39 °C, but was maintained between 25°C and 55°C. LyJH307 bactericidal action was retained under 0-500 mM NaCl. While the activity of LyJH307 significantly decreased on treatment with ethylenediaminetetraacetic acid (EDTA), it was only restored with supplementation of 10 mM Ca2+. Analyses of antimicrobial spectra showed that LyJH307 lysed Lancefield groups D (S. bovis group and Enterococcus faecalis) and H (S. sanguinis) bacteria. Thus, LyJH307 might help to prevent acute ruminal acidosis.


2016 ◽  
Vol 12 (6) ◽  
pp. 602-611 ◽  
Author(s):  
O. Sotelo-Mazón ◽  
Cecilia Cuevas-Arteaga ◽  
J. Porcayo-Calderón ◽  
G. Izquierdo-Montalvo

2021 ◽  
pp. 138502
Author(s):  
Yuanming Wang ◽  
Yunhong Song ◽  
Chunling Ma ◽  
Hong-qi Xia ◽  
Ranran Wu ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document