scholarly journals Highly‐Parallel Microfluidics‐Based Force Spectroscopy on Single Cytoskeletal Motors

Small ◽  
2021 ◽  
pp. 2007388
Author(s):  
Marta Urbanska ◽  
Annemarie Lüdecke ◽  
Wilhelm J. Walter ◽  
Antoine M. Oijen ◽  
Karl E. Duderstadt ◽  
...  
Keyword(s):  
Force Spectroscopy ◽  
Cytoskeletal Motors

scholarly journals Highly-parallel microfluidics-based force spectroscopy on single cytoskeletal motors

2020 ◽  
Author(s):  
Marta Urbanska ◽  
Annemarie Lüdecke ◽  
Wim J. Walter ◽  
Antoine M. van Oijen ◽  
Karl E. Duderstadt ◽  
...  
Keyword(s):  
Single Molecule ◽  
Vertical Component ◽  
Force Spectroscopy ◽  
Molecular Machines ◽  
Field Of View ◽  
Large Field ◽  
Cellular Functions ◽  
Molecular Systems ◽  
Cytoskeletal Motors ◽  
Run Lengths

AbstractCytoskeletal motors transform chemical energy into mechanical work to drive essential cellular functions. Optical trapping experiments have provided crucial insights into the operation of these molecular machines under load. However, the throughput of such force spectroscopy experiments is typically limited to one measurement at a time. Here, we describe an alternative, highly-parallel, microfluidics-based method that allows for rapid collection of force-dependent motility parameters of cytoskeletal motors. We applied tunable hydrodynamic forces to stepping kinesin-1 motors via DNA-tethered beads and utilized a large field-of-view to simultaneously track the velocities, run lengths and interaction times of hundreds of individual kinesin-1 molecules under varying resisting and assisting loads. Importantly, the 16-μm long DNA tethers between the motors and the beads significantly reduced the vertical component of the applied force pulling the motors away from the microtubule. Our approach is readily applicable to other molecular systems and constitutes a new methodology for parallelized single-molecule force studies on cytoskeletal motors.

Dysfunction of endothelial cells exposed to nanomaterials assessed by atomic force spectroscopy

Micron ◽  
2021 ◽  
Vol 145 ◽  
pp. 103062
Author(s):  
Agnieszka Maria Kolodziejczyk ◽  
Paulina Sokolowska ◽  
Aleksandra Zimon ◽  
Magdalena Grala ◽  
Marcin Rosowski ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Force Spectroscopy ◽  
Atomic Force Spectroscopy ◽  
Atomic Force

scholarly journals Single Molecule Force Spectroscopy of Siloxanes

2021 ◽  
Vol 714 (3) ◽  
pp. 032023
Author(s):  
Ling Chen ◽  
Liya Yang ◽  
Chunxia Wang ◽  
Ting Zhu
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

scholarly journals Determination of the molar mass of surface-grafted weak polyelectrolyte brushes using force spectroscopy

Polymer ◽  
2015 ◽  
Vol 67 ◽  
pp. 111-117 ◽  
Author(s):  
Ateyyah AL-Baradi ◽  
Michael R. Tomlinson ◽  
Zhenyu J. Zhang ◽  
Mark Geoghegan
Keyword(s):  
Molar Mass ◽  
Force Spectroscopy ◽  
Polyelectrolyte Brushes

scholarly journals Nanomechanics of self-assembled DNA building blocks

Nanoscale ◽  
2021 ◽  
Author(s):  
Michael Penth ◽  
Kordula Schellnhuber ◽  
Roland Bennewitz ◽  
Johanna Blass
Keyword(s):  
Structural Dynamics ◽  
Force Spectroscopy ◽  
Building Blocks ◽  
Dna Origami ◽  
Self Assembled ◽  
High Flexibility

Massive parallel force spectroscopy reveals a surprisingly high flexibility for DNA constructs used in DNA origami. The high flexibility is attributed to the structural dynamics of DNA self-assemblies.

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