Clinically Approved Carbon Nanoparticles with Oral Administration for Intestinal Radioprotection via Protecting the Small Intestinal Crypt Stem Cells and Maintaining the Balance of Intestinal Flora

Small ◽  
2020 ◽  
Vol 16 (16) ◽  
pp. 1906915 ◽  
Author(s):  
Chengyan Wang ◽  
Jiani Xie ◽  
Xinghua Dong ◽  
Linqiang Mei ◽  
Maoru Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Oral Administration ◽  
Carbon Nanoparticles ◽  
Intestinal Flora ◽  
Intestinal Crypt ◽  
Small Intestinal

scholarly journals A novel in vitro survival assay of small intestinal stem cells after exposure to ionizing radiation

2014 ◽  
Vol 55 (2) ◽  
pp. 381-390 ◽  
Author(s):  
Motohiro Yamauchi ◽  
Kensuke Otsuka ◽  
Hisayoshi Kondo ◽  
Nobuyuki Hamada ◽  
Masanori Tomita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ionizing Radiation ◽  
Intestinal Stem Cells ◽  
Survival Assay ◽  
Small Intestinal ◽  
In Vitro Survival

Heterogeneity in histone 2B-green fluorescent protein-retaining putative small intestinal stem cells at cell position 4 and their absence in the colon

2012 ◽  
Vol 303 (11) ◽  
pp. G1188-G1201 ◽  
Author(s):  
Kevin R. Hughes ◽  
Ricardo M. C. Gândara ◽  
Tanvi Javkar ◽  
Fred Sablitzky ◽  
Hanno Hock ◽  
...  
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Paneth Cells ◽  
Epithelial Stem Cells ◽  
Epithelial Regeneration ◽  
Cell Position ◽  
Small Intestinal ◽  
Green Fluorescent ◽  
Dose Radiation

Stem cells have been identified in two locations in small intestinal crypts; those intercalated between Paneth cells and another population (which retains DNA label) are located above the Paneth cell zone, at cell position 4. Because of disadvantages associated with the use of DNA label, doxycycline-induced transient transgenic expression of histone 2B (H2B)-green fluorescent protein (GFP) was investigated. H2B-GFP-retaining putative stem cells were consistently seen, with a peak at cell position 4, over chase periods of up to 112 days. After a 28-day chase, a subpopulation of the H2B-GFP-retaining cells was cycling, but the slow cycling status of the majority was illustrated by lack of expression of pHistone H3 and Ki67. Although some H2B-GFP-retaining cells were sensitive to low-dose radiation, the majority was resistant to low- and high-dose radiation-induced cell death, and a proportion of the surviving cells proliferated during subsequent epithelial regeneration. Long-term retention of H2B-GFP in a subpopulation of small intestinal Paneth cells was also seen, implying that they are long lived. In contrast to the small intestine, H2B-GFP-retaining epithelial cells were not seen in the colon from 28-day chase onward. This implies important differences in stem cell function between these two regions of the gastrointestinal tract, which may have implications for region-specific susceptibility to diseases (such as cancer and ulcerative colitis), in which epithelial stem cells and their progeny are involved.

scholarly journals Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells

2017 ◽  
Vol 31 (S1) ◽  
Author(s):  
Kunihiro Kishida ◽  
Sarah Pearce ◽  
Shiyan Yu ◽  
Nan Gao ◽  
Ronaldo Ferraris
Keyword(s):  
Stem Cells ◽  
Nutrient Sensing ◽  
Intestinal Stem Cells ◽  
Small Intestinal

scholarly journals In Vitro Blood-Brain-Barrier Permeability and Cytotoxicity of Atorvastatin-Loaded Nanoformulation Against Glioblastoma in 2D and 3D Models

2019 ◽  
Author(s):  
Michael M Lübtow ◽  
Sabrina Oertner ◽  
Sabina Quader ◽  
Elisabeth Jeanclos ◽  
Alevtina Cubukova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Oral Administration ◽  
Blood Brain Barrier ◽  
Cell Lines ◽  
Drug Loading ◽  
3D Models ◽  
Physicochemical Characteristics ◽  
Brain Barrier ◽  
Blood Brain

Inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase of the family of statins have been suggested as therapeutic options in various tumors. Atorvastatin is a statin with potential to cross the blood-brain-barrier, however, the concentrations necessary for a cytotoxic effect against cancer cells exceeds the concentration achievable via oral administration, which made the development of a novel atorvastatin formulation necessary. We characterized the drug loading and basic physicochemical characteristics of micellar atorvastatin formulations and tested their cytotoxicity against a panel of different glioblastoma cell lines. In addition, activity against tumor spheroids formed from mouse glioma and mouse cancer stem cells, respectively, was evaluated. Our results show good activity of atorvastatin against all tested cell lines. Interestingly, in the 3D models, growth inhibition was more pronounced for the micellar formulation compared to free atorvastatin. Finally, atorvastatin penetration across a blood-brain-barrier model obtained from human induced-pluripotent stem cells was evaluated. Our results suggest that the presented micelles may enable much higher serum concentrations than possible by oral administration, however, if transport across the blood-brain-barrier is sufficient to reach therapeutic atorvastatin concentration for the treatment of glioblastoma via intravenous administration remains unclear.<br>

Age Changes in Stem Cells of Murine Small Intestinal Crypts

1998 ◽  
Vol 241 (2) ◽  
pp. 316-323 ◽  
Author(s):  
K. Martin ◽  
T.B.L. Kirkwood ◽  
C.S. Potten
Keyword(s):  
Stem Cells ◽  
Age Changes ◽  
Small Intestinal

scholarly journals ALTERATIONS IN THE INTESTINAL FLORA WITH ORAL ADMINISTRATION OF ANTIBIOTICS

1958 ◽  
Vol 2 (3) ◽  
pp. 225-230 ◽  
Author(s):  
Shogo Kuwahara ◽  
Hiroshi Nakahara ◽  
Seiichiro Yasushi ◽  
Kikuno Ota ◽  
Mie Takano
Keyword(s):  
Oral Administration ◽  
Intestinal Flora

Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (5) ◽  
pp. 1317-1326 ◽  
Author(s):  
Shaofeng Wu ◽  
Yan Liu ◽  
Shantaram Bharadwaj ◽  
Anthony Atala ◽  
Yuanyuan Zhang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Human Urine ◽  
Small Intestinal Submucosa ◽  
Small Intestinal ◽  
Intestinal Submucosa
Sign in / Sign up

Export Citation Format

Share Document